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Preparation method and application of nanogold composite material immunosensor

A technology of immune sensor and composite material, which is applied in the field of preparation of nano-gold composite material immune sensor, can solve the problems of low efficiency of nano-gold, and achieve the effects of good water solubility, improved utilization rate and good activity

Inactive Publication Date: 2015-05-20
QUFU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the efficiency of using nano-gold as a catalyst in biosensors is still low

Method used

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  • Preparation method and application of nanogold composite material immunosensor
  • Preparation method and application of nanogold composite material immunosensor
  • Preparation method and application of nanogold composite material immunosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Preparation of aminated Fe 3 o 4 Nano magnetic beads

[0039] 0.325g anhydrous FeCl 3 and 0.2 g of sodium citrate were dispersed in 20 mL of ethylene glycol, stirred for 1 h to dissolve the solid completely, then 1.2 g of sodium acetate was added and stirred for 30 min, the mixture was added to a hydrothermal reaction kettle with a volume of 50 mL, and placed in an oven, Keep at 200°C for 12 hours, and when the reactor is cooled to room temperature, take it out, and the black precipitate at the bottom of the reactor is Fe 3 o 4 Nano-magnetic beads, using a magnet for magnetic separation, the obtained Fe 3 o 4 Washed three times with water and ethanol respectively, dried in vacuum at 50°C for 12 hours, and obtained 4 mg Fe 3 o 4 Disperse in 10 mL of water, add 20 μL of 3-aminopropyltriethoxysilane, and stir at room temperature for 6 hours to obtain aminated Fe 3 o 4 nano magnetic beads;

[0040] (2) Preparation of hollow polydopamine-gold nanomaterials (PDA...

Embodiment 2

[0051] (1) Preparation of aminated Fe 3 o 4 Nano magnetic beads

[0052] 0.325g anhydrous FeCl 3 Disperse with 0.2g sodium citrate in 20mL ethylene glycol, stir for 1h to completely dissolve the solid; then add 1.2g sodium acetate and stir for 30min. The mixture was added into a hydrothermal reaction kettle with a volume of 50 mL, put into an oven, and kept at 200 °C for 12 h, and was taken out when the reaction kettle was cooled to room temperature. The black precipitate at the bottom of the reaction kettle is Fe 3 o 4 Nano-magnetic beads, using a magnet for magnetic separation, the obtained Fe 3 o 4 Wash with water and ethanol three times respectively, and dry in vacuum at 50°C for 12h. Take 4mg Fe 3 o 4 Disperse in 10 mL of water, add 20 μL of 3-aminopropyltriethoxysilane, stir at room temperature for 6 h, and the obtained aminated Fe 3 o 4 nano magnetic beads;

[0053] (2) Preparation of hollow polydopamine-gold nanomaterials (PDA-Au)

[0054] Weigh 40 mg Fe ...

Embodiment 3

[0064] (1) Preparation of aminated Fe 3 o 4 Nano magnetic beads

[0065] 0.325g anhydrous FeCl 3 and 0.2 g of sodium citrate were dispersed in 20 mL of ethylene glycol, stirred for 1 h to completely dissolve the solid, then added 1.2 g of sodium acetate and stirred for 30 min, and the mixture was added to a hydrothermal reaction kettle with a volume of 50 mL, and placed in an oven , Keep at 200°C for 12h, and take it out when the reactor is cooled to room temperature. The black precipitate at the bottom of the reaction kettle is Fe 3 o 4 Nano-magnetic beads, using a magnet for magnetic separation, the obtained Fe 3 o 4 Wash with water and ethanol three times respectively, and dry in vacuum at 50°C for 12h. Take 4mg Fe 3 o 4 Disperse in 10mL water, add 20μL 3-aminopropyltriethoxysilane, stir at room temperature for 6h, and the obtained aminated Fe 3 o 4 nano magnetic beads;

[0066] (2) Preparation of hollow polydopamine-gold nanomaterials (PDA-Au)

[0067] Weigh 4...

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Abstract

The invention provides a preparation method and an application of a nanogold composite material immunosensor. The method comprises the following steps: dispersing aminated Fe3O4 nano magnetic beads into a Tris buffer solution and connecting with a cancer biomarker antibody (Ab1) as an easy-to-separate immunoprobe; dispersing a hollow polydopamine-nanogold material (PDA-Au) into the Tris buffer solution and connecting with a cancer biomarker antibody (Ab2) as an immune recognition material. Ab1 and Ab2 are specifically combined with a corresponding cancer marker antigen to form a sandwich immune system. The immune system is separated out through the magnetic property, then is added to a mixed water solution of p-nitrophenol and sodium borohydride, and is determined through an ultraviolet visible instrument. The linear range of immune detection of the nanogold composite material immunosensor prepared by the method is 0-100U / mL; the detection lower limit reaches 0.05U / mL; and the related coefficient reaches 0.998.

Description

technical field [0001] The invention belongs to the field of sensors, and in particular relates to a preparation method and application of a nano-gold composite immune sensor. Background technique [0002] Due to its small size effect, surface effect, and controllable particle size, gold nanoparticles have been widely used in biomedical engineering, electronics, and catalytic engineering. Loading nano-gold on different carriers can form a series of new nano-gold catalysts, which can be used in different catalytic systems. At the same time, due to its biocompatibility, gold nanoparticles have been widely used in the research and development of biosensors. In the process of development and improvement of biosensors, the main work focuses on improving signal sensitivity and reducing the lower limit of detection. At present, the efficiency of using nano-gold as a catalyst in biosensors is still low. Contents of the invention [0003] The technical problem to be solved by the...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/531
CPCG01N33/54346G01N33/574G01N2800/7028
Inventor 渠凤丽赵岩
Owner QUFU NORMAL UNIV
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