Fermented cordyceps sinensis derived beta-glucan extract and preparation method thereof

A technology of fermenting Cordyceps and dextran, which is applied in food preparation, food science, application, etc., can solve the problem of loss of interest in fungal polysaccharides and achieve the effect of reducing the dosage

Active Publication Date: 2015-05-27
JIANGSU SHENHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because domestic research on Cordyceps polysaccharides is still at a relatively low level, some manufacturers even one-sidedly pursue the total polysac

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0054] Example 1

[0055] a) To the fermented wet cordyceps mycelium, add ethanol with a mass concentration of 80%, heat to 30° C., keep for 4 hours, separate by a decanter centrifuge at 3000 r / min, discard the filtrate, and use the wet mycelium for use.

[0056] b) Add the wet mycelium in a), add purified water, the ratio of the bacterial cell to the purified water is 1:3, stir for 5-30min, the rotation speed is 10r / min-100r / min, then the decanter centrifuge at 3000r Centrifuge for 1h under the condition of / min, discard the filtrate, and obtain the washed mycelium.

[0057] c) Add purified water to the mycelium washed in b). The weight ratio of mycelium to purified water is 1:3, boil it, keep it for 6h, every 1h, stir at 10r / min for 10min, decanter centrifuge Separate by machine, discard mycelium, and keep the filtrate.

[0058] d) The filtrate obtained in c), the vacuum degree is 0.02MPa-0.10MPa, at 60℃, double-effect concentration to a specific gravity of 0,8-1.3, into the alcoho...

Example Embodiment

[0064] Example 2

[0065] a) The fermented wet cordyceps mycelium is added with ethanol with a mass concentration of 70%, heated to 40°C, kept for 2h, and separated by a decanter centrifuge under the condition of 1000r / min. The filtrate is discarded and the wet mycelium is used for later use.

[0066] b) Add the wet mycelium in a), add purified water, the ratio of the bacterial cell to the purified water is 1:5, stir for 30min, the speed is 30r / min, then centrifuge at 1000r / min in a decanter for 1.5h , Discard the filtrate to obtain washed mycelium.

[0067] c) Add purified water to the mycelium washed in b), the weight ratio of mycelium to purified water is 1:5, boil, keep for 3h, every 1h, stir at 10r / min for 5min, decanter centrifuge Separate by machine, discard mycelium, and keep the filtrate.

[0068] d) The filtrate obtained in c), vacuum degree 0.02MPa-0.10MPa, double-effect concentration at 40-50℃ to a specific gravity of 0,8-1.3, into the alcohol precipitation pot, while sti...

Example Embodiment

[0073] Example 3

[0074] The β-glucan extract prepared by the method in Example 1, sodium chloride and coffee powder in a weight ratio of 1:0.05-0.30:10-30 were mixed with a three-dimensional motion mixer for 60-90 minutes, that is, β-glucan Sugar coffee.

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Abstract

The invention discloses a fermented cordyceps sinensis derived beta-glucan extract and a preparation method thereof. The extract contains more than 50% of total polysaccharides, more than 40% of 1,3-1,6 beta glucan, and less than 10% of alpha glucan. The preparation method can be used for effectively extracting effective ingredients; a prepared product has concentrated beneficial ingredients of fermented cordyceps sinensis and can be used as a health product or drug, and the quantity of intaking can be obviously reduced.

Description

technical field [0001] The invention belongs to the application field of fungal resources, and relates to a preparation method of fermented cordyceps-derived β-glucan extract. Background technique [0002] β-glucan is a polysaccharide with multiple functional activities, the main chemical structure is β-1,3 glucan and β-1,6 glucan, the former has anti-tumor properties, and can greatly improve the human body natural immunity. [0003] The results of research by Japanese scientists have shown that β-glucan can regulate blood sugar levels and activate insulin activity, bringing good news to patients with hyperglycemia. Because domestic research on Cordyceps polysaccharides is still at a relatively low level, some manufacturers even one-sidedly pursue the total polysaccharide content and add maltodextrin, β-cyclodextrin and yellow dextrin, resulting in the loss of interest in fungal polysaccharides at home and abroad. [0004] In order to further develop and utilize fermented ...

Claims

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Application Information

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IPC IPC(8): A23L1/28A23L1/29A23L31/00
Inventor 王希艳徐国华黄盟盟张芙蓉王英燕李荀
Owner JIANGSU SHENHUA PHARMA
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