Beta-carotene-rich lactobacillus fermented functional fruit-vegetable beverage and preparation method thereof
A technology of lactic acid bacteria fermentation and carotene, which is applied in the biological field to achieve the effect of simple operation and comprehensive and reasonable nutrition
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Embodiment 1
[0034] The preparation of embodiment 1 fruit and vegetable puree
[0035] Carrot puree: clean the raw carrots, peel them, cut them into thin slices of 0.2 mm, add water of the same quality as the carrots, boil and beat, cool to room temperature, add 0.4 g / L pectinase for enzymatic hydrolysis, and obtain carrots Puree.
[0036] Tomato puree: Wash the tomatoes, blanching them with hot water above 90°C for 3 minutes, peel off the outer skin, cut into small pieces and beat, cool to room temperature, add 0.2g / L pectinase for enzymatic hydrolysis to obtain tomato puree.
[0037] Watermelon puree: wash the watermelon, peel it, cut it into small pieces and beat it, add 0.1g / L pectinase for enzymatic hydrolysis to obtain watermelon puree.
Embodiment 2
[0038] The selection of embodiment 2 fermenting lactic acid bacteria
[0039] The carrot puree prepared in Example 1, the tomato puree, and the watermelon puree were mixed in a volume ratio of 4:3:4 as fermentation raw materials for subsequent use. Add lactic acid bacteria to ferment according to the inoculation amount of 5%, said lactic acid bacteria add according to the ratio shown in table 1, refine and ferment in 37 ℃ constant temperature incubator, take the comprehensive sensory score of fermented fruit and vegetable beverage as index (according to the evaluation index of table 2 ), and the results obtained are shown in Table 1.
[0040] Table 1 Fermentation results of different combinations of lactic acid bacteria
[0041]
Embodiment 3
[0042] Embodiment 3 Preparation of lactic acid bacteria fermented functional fruit and vegetable beverage
[0043] (1) Sensory evaluation standard
[0044] Sensory analysis was carried out on the fermented fruit and vegetable beverage, and the scoring method was used to score the color, aroma, taste, clarity and overall feeling. The specific scoring table is shown in Table 2. The final score is the sum of all items, and the sensory score is judged by 10 graduate students with tasting experience in the research group.
[0045] Table 2 Sensory evaluation indicators of fermented fruit and vegetable beverages
[0046]
[0047] (2) Activation of bacteria
[0048] The lactic acid bacteria are connected with 1% peptone, 1% beef extract, 0.5% yeast extract, 2% glucose, 0.2% ammonium citrate, 0.5% sodium acetate, 0.5% dipotassium hydrogen phosphate, 0.02% manganese sulfate, and 0.05% magnesium sulfate , in the MRS medium (pH 6.0±0.2) prepared by Tween-800.1%, after culturing for ...
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