Method for acquiring bioactive proteins by utilizing umbilical cord mesenchymal stem cells

A bioactive protein and stem cell technology, which is applied in the field of obtaining bioactive protein by using umbilical cord mesenchymal stem cells, can solve the problems of increasing the risk of animal-derived components, reducing the safety of clinical application, and difficult monitoring of the culture medium state, etc., to achieve Reduce the attachment time, increase the cell growth rate, and improve the effect of cell stability

Inactive Publication Date: 2015-05-27
SHENYANG BESTSTEM BIOTECH
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Problems solved by technology

Using bioreactors to cultivate cells to obtain bioactive proteins to meet the needs of clinical applications is a major trend in the production of stem cells and bioactive proteins, but there are many shortcomings in practical applications: patent CN101985612A uses microcarriers in culture bags For large-scale culture of adherent cells, there are disadvantages that the cells are easy to settle and the state of the cu

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  • Method for acquiring bioactive proteins by utilizing umbilical cord mesenchymal stem cells
  • Method for acquiring bioactive proteins by utilizing umbilical cord mesenchymal stem cells
  • Method for acquiring bioactive proteins by utilizing umbilical cord mesenchymal stem cells

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Embodiment Construction

[0046] The present invention will be further described in detail below in conjunction with specific examples.

[0047] The invention provides a method for obtaining bioactive protein by using umbilical cord mesenchymal stem cells, the specific steps are as follows.

[0048] Step 1. Collection and transportation of umbilical cord.

[0049] With the informed consent of the puerpera, fresh aseptic umbilical cord tissue was collected from full-term healthy fetuses, stored at 4°C, and processed within 24 hours. Collect maternal blood within seven days before and after the umbilical cord collection for HIV testing, hepatitis B testing, hepatitis C testing, syphilis testing, alanine aminotransferase testing, and mycoplasma testing.

[0050] Cleaning and disinfection: Take out the umbilical cord in the biosafety cabinet, first clean the surface with 0.9% sodium chloride injection, and then soak the umbilical cord in 75% alcohol for no more than 2 minutes, and the alcohol is filtered ...

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Abstract

The invention discloses a method for acquiring bioactive proteins by utilizing umbilical cord mesenchymal stem cells. The method comprises the following steps: digesting Wharton's jelly of umbilical cord in short time by using type I collagenase, thereby obtaining a paste mixture containing extracellular matrix proteins and tissue blocks; performing primary culture; adding a stem cell growth culture medium into a packed bed type bioreactor for amplifying the umbilical cord mesenchymal stem cells; adding a protein production culture medium for performing continuous culture when the cell fusion rate in the reactor reaches the standard; collecting the culture solution, and treating the solution, thereby obtaining the bioactive proteins. According to the method for acquiring the bioactive proteins by utilizing umbilical cord mesenchymal stem cells disclosed by the invention, the purity of the obtained umbilical cord mesenchymal stem cells is high, the multiplication capacity is high, and the stem cell culture time is shortened; and moreover, the yield of the obtained bioactive proteins is high, the quality control process is convenient, and large-scale production is easily realized. The stem cells and bioactive proteins do not contain animal source components, and the safety of clinical application is improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for obtaining biologically active proteins by using umbilical cord mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (Mesenchymal stem cells) are a type of adult stem cells with multi-directional differentiation potential. obtained from various organizations. Mesenchymal stem cells are non-terminal differentiated cells, which have both the characteristics of mesenchymal cells and the characteristics of endothelial cells and epithelial cells; as a type of pluripotent stem cells, they can develop into bone, Cartilage, muscle, tendon, liver, fat, nerve, endothelial and islet-like cells proliferate and differentiate. Currently known to have certain therapeutic effects on myocardial infarction, diabetes, osteoporosis, osteonecrosis, lupus nephritis, liver cirrhosis, liver failure, multiple cirrhosis, systemic lupus erythematosus, Parkinson's disease, ...

Claims

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Application Information

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IPC IPC(8): C12N5/0775C12P21/02
Inventor 时兆田于艳秋荣耀星张停停邓雯雯李秀文吕亚楠
Owner SHENYANG BESTSTEM BIOTECH
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