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Paenibacillus polymyxa JX-13 and application thereof

A technology of Paenibacillus polymyxa and Bacillus, which is applied in the application and preparation of preparations, Paenibacillus polymyxa JX-13, microorganisms and application fields, can solve the problems not mentioned and disclosed, and achieve low production cost, high fermentation Simple process and good control effect

Active Publication Date: 2015-06-10
南京金色庄园农产品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Paenibacillus polymyxa antagonist JX-13 bacterial strain provided by the present invention and preparation, the application on the prevention and treatment of disease on strawberry, grape is not mentioned and disclosed in the prior art

Method used

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  • Paenibacillus polymyxa JX-13 and application thereof
  • Paenibacillus polymyxa JX-13 and application thereof
  • Paenibacillus polymyxa JX-13 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The method for obtaining Paenibacillus polymyxa (Paenibacillus polymyxa) JX-13 bacterial strain is as follows:

[0026] The PDA medium formula is: potato (peeled) 200g / L, glucose 20g / L, water 1L, agar 20g / L, pH natural. Peel the potatoes, cut them into small pieces, add about 1L of water to the pot and boil for half an hour, filter with double gauze, take the filtrate, add glucose, and add water to make up 1L.

[0027] The method for obtaining the bacterial strain comprises the following steps:

[0028] 1) Isolation, purification and preservation of strains.

[0029] Samples were collected from the root soil of Photinia macrophylla in the Agricultural Expo Park of Jiangsu Agriculture and Forestry Vocational and Technical College located in Chenwu Town, Jurong City, Jiangsu Province. The samples were diluted with sterile water on an ultra-clean workbench and spread on PDA plates And place it in a 30°C incubator for cultivation. According to the characteristics of the b...

Embodiment 2

[0039] The wettable powder prepared by bacterial strain JX-13 of the present invention, its preparation method comprises the following steps:

[0040] (A) Obtaining the bacterial solution

[0041] Pick the single colony of JX-13 strain that has been streaked and cultured on the PDA solid medium in advance with a sterile bud stick, and inoculate it in a 20mL Erlenmeyer flask containing 5mL of PDA liquid culture medium, at 30°C, 200rpm Under the conditions of shaking culture for 16 hours, overnight, inoculate all 5 mL of the culture solution obtained in the previous step into a 1000 mL Erlenmeyer flask containing 400 mL of PDA culture solution, shake and culture at 200 rpm and 30 ° C for 16 hours; inoculate the obtained 400 mL culture solution In a 30L fermenter (Zhenjiang Dongfang GUS-30) with a 20L culture medium, the solid contents in the 20L fermentation medium are: soybean meal 100g, potato starch 200g, sucrose 25g, yeast powder 25g, CaCO 3 20g, MnSO 4 1.0g, set the ferment...

experiment example

[0046] Field Control Experiment of JX-13 WP on Strawberry Diseases

[0047] The field control effect test was carried out in the greenhouse strawberry garden and vineyard in Baitu Town, Jurong City, Jiangsu Province. The strawberry variety was Hongchee and the grape variety was Kyoho. The experimental treatment plan is as follows:

[0048] (A) Prevention and treatment of strawberry wilt

[0049] Chemical treatment method: Paenibacillus polymyxa JX-13 wettable powder (10 billion live spores / gram) 1000 times liquid, contrast agent 50% thiophanate-methyl wettable powder 1000 times liquid, when transplanting strawberries Roots of seedlings were irrigated with 200ml medicinal solution, blank control was irrigated with clear water, 200 seedlings per plot, arranged in random blocks, repeated 3 times, and the disease index was investigated 60 days after root irrigation.

[0050] (B) Control of strawberry anthracnose and grape anthracnose

[0051] Test agent treatment: Paenibacillus...

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Abstract

The invention discloses paenibacillus polymyxa JX-13 of which the classification is named as paenibacillus polymyxa. The paenibacillus polymyxa JX-13 is preserved in the common microorganism center of the Chinese microorganism culture preservation management committee, the preservation number is CGMCC No.9796, and the preservation date is October 17, 2014. The invention further discloses the application of the paenibacillus polymyxa JX-13. According to the paenibacillus polymyxa JX-13, wettable powder prepared by the fermentation of the strain can effectively prevent and control diseases such as strawberry blight, anthracnose and grey mold and grape grey mold and anthracnose. The storing property of the bacterium agent is stable, the control effect is good, and the mass production fermentation technology is simple and low in production cost.

Description

technical field [0001] The invention relates to a microorganism and its use, more specifically to a Paenibacillus polymyxa JX-13, its use and a preparation method for its preparation, belonging to the technical field of biological pesticides. Background technique [0002] At present, the control of crop diseases still mainly relies on chemical pesticides. The resulting increase in the resistance of pathogenic bacteria, environmental pollution and pesticide residues in fruits have become increasingly prominent. The search for safe and effective biological control measures to control diseases has attracted increasing attention. Soil-borne diseases such as strawberry wilt are devastating root diseases that are difficult to control in strawberry production at home and abroad. They are mainly caused by the combined infection of soil-borne pathogenic fungi such as Fusarium oxysporum. The growth and development of affected plants are hindered, and the yield and quality The serious ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N25/14A01P3/00C12R1/01
CPCA01N25/14A01N63/00A01N63/10C12N1/205C12R2001/01
Inventor 吉沐祥陈宏州吴祥赵桂华杨敬辉郭建毛妮妮
Owner 南京金色庄园农产品有限公司
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