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A kind of Paenibacillus polymyxa jx‑13 and its application

A technology of Paenibacillus polymyxa and bacilli, applied in the field of microorganisms and their uses, uses and preparations thereof, and the field of Paenibacillus polymyxa JX-13, which can solve the problems that have not been mentioned and disclosed, and achieve low production costs and high fermentation The effect of simple process and stable storage properties

Active Publication Date: 2017-10-31
南京金色庄园农产品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Paenibacillus polymyxa antagonist JX-13 bacterial strain provided by the present invention and preparation, the application on the prevention and treatment of disease on strawberry, grape is not mentioned and disclosed in the prior art

Method used

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  • A kind of Paenibacillus polymyxa jx‑13 and its application
  • A kind of Paenibacillus polymyxa jx‑13 and its application
  • A kind of Paenibacillus polymyxa jx‑13 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The method for obtaining Paenibacillus polymyxa (Paenibacillus polymyxa) JX-13 bacterial strain is as follows:

[0026] The PDA medium formula is: potato (peeled) 200g / L, glucose 20g / L, water 1L, agar 20g / L, pH natural. Peel the potatoes, cut them into small pieces, add about 1L of water to the pot and boil for half an hour, filter with double gauze, take the filtrate, add glucose, and add water to make up 1L.

[0027] The method for obtaining the bacterial strain comprises the following steps:

[0028] 1) Isolation, purification and preservation of strains.

[0029] Samples were collected from the root soil of Photinia macrophylla in the Agricultural Expo Park of Jiangsu Agriculture and Forestry Vocational and Technical College located in Chenwu Town, Jurong City, Jiangsu Province. The samples were diluted with sterile water on an ultra-clean workbench and spread on PDA plates And place it in a 30°C incubator for cultivation. According to the characteristics of the b...

Embodiment 2

[0039] The wettable powder prepared by bacterial strain JX-13 of the present invention, its preparation method comprises the following steps:

[0040] (A) Obtaining the bacterial solution

[0041] Pick the single colony of JX-13 strain that has been streaked and cultured on the PDA solid medium in advance with a sterile bud stick, and inoculate it in a 20mL Erlenmeyer flask containing 5mL of PDA liquid culture medium, at 30°C, 200rpm Under the conditions of shaking culture for 16 hours, overnight, inoculate all 5 mL of the culture solution obtained in the previous step into a 1000 mL Erlenmeyer flask containing 400 mL of PDA culture solution, shake and culture at 200 rpm and 30 ° C for 16 hours; inoculate the obtained 400 mL culture solution In a 30L fermenter (Zhenjiang Dongfang GUS-30) with a 20L culture medium, the solid contents in the 20L fermentation medium are: soybean meal 100g, potato starch 200g, sucrose 25g, yeast powder 25g, CaCO 3 20g, MnSO 4 1.0g, set the ferment...

experiment example

[0046] Field Control Experiment of JX-13 WP on Strawberry Diseases

[0047] The field control effect test was carried out in the greenhouse strawberry garden and vineyard in Baitu Town, Jurong City, Jiangsu Province. The strawberry variety was Hongchee and the grape variety was Kyoho. The experimental treatment plan is as follows:

[0048] (A) Prevention and treatment of strawberry wilt

[0049] Chemical treatment method: Paenibacillus polymyxa JX-13 wettable powder (10 billion live spores / gram) 1000 times liquid, contrast agent 50% thiophanate-methyl wettable powder 1000 times liquid, when transplanting strawberries Roots of seedlings were irrigated with 200ml medicinal solution, blank control was irrigated with clear water, 200 seedlings per plot, arranged in random blocks, repeated 3 times, and the disease index was investigated 60 days after root irrigation.

[0050] (B) Control of strawberry anthracnose and grape anthracnose

[0051] Test agent treatment: Paenibacillus...

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Abstract

The invention discloses a Paenibacillus polymyxa JX-13, which is classified as Paenibacillus polymyxa, and is preserved in the General Microorganism Center of the China Committee for the Preservation of Microorganisms, and the preservation number is: CGMCC No.9796 , the date of deposit is October 17, 2014. The invention also discloses the application of the above-mentioned Paenibacillus polymyxa JX-13. The Paenibacillus polymyxa JX-13 of the present invention, and the wettable powder obtained by fermenting the strain, can effectively prevent and cure diseases such as strawberry wilt, anthracnose, gray mold, grape gray mold, anthracnose and the like. The bacterial agent has stable storage properties, good control effect, simple fermentation process for large-scale production, and low production cost.

Description

technical field [0001] The invention relates to a microorganism and its use, more specifically to a Paenibacillus polymyxa JX-13, its use and a preparation method for its preparation, belonging to the technical field of biological pesticides. Background technique [0002] At present, the control of crop diseases still mainly relies on chemical pesticides. The resulting increase in the resistance of pathogenic bacteria, environmental pollution and pesticide residues in fruits have become increasingly prominent. The search for safe and effective biological control measures to control diseases has attracted increasing attention. Soil-borne diseases such as strawberry wilt are devastating root diseases that are difficult to control in strawberry production at home and abroad. They are mainly caused by the combined infection of soil-borne pathogenic fungi such as Fusarium oxysporum. The growth and development of affected plants are hindered, and the yield and quality The serious ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01N25/14A01P3/00C12R1/01
CPCA01N25/14A01N63/00A01N63/10C12N1/205C12R2001/01
Inventor 吉沐祥陈宏州吴祥赵桂华杨敬辉郭建毛妮妮
Owner 南京金色庄园农产品有限公司
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