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Swordlike atractylodes rhizome tissue culture and rapid propagation method

A technology of tissue culture rapid propagation and Atractylodes atractylodes, which is applied in the field of plant tissue culture, can solve the problems of Atractylodes atractylodis species mixing, restricting rapid breeding, new variety selection, and degeneration of varieties, so as to expand the speed and scale of reproduction, protect wild Atractylodes atractylodes resources, The effect of mitigating real problems

Inactive Publication Date: 2015-06-24
罗焕荣
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the "SARS" in 2003, the traditional Chinese medicinal herb Atractylodes atractylodis has gradually been valued by pharmaceutical companies, drug markets, and drug dealers.
However, in the process of cultivation, long-term asexual breeding has caused problems such as degradation of medicinal value of Atractylodes atractylodes, aggravation of diseases, and decline in yield. In addition, Atractylodes atractylodis is a variable variety with a wide variety of varieties, which makes the artificially cultivated Atractylodes atractylodes varieties seriously mixed and the quality is uneven. uneven
At present, how to solve the provenance of Atractylodes atractylodes and improve the resistance of the variety, and expand the planting scale of Atractylodes atractylodes are practical problems that need to be solved urgently in the artificial cultivation of Atractylodes atractylodes. Rapid breeding and new breed selection and other work

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Induction culture: After the collected stems are washed with clear water to remove surface attachments, soak in laundry detergent for 5 minutes, then rinse with running water for 1 hour, disinfect with 75% ethanol solution for 10 seconds in an ultra-clean workbench, and then wash with sterile water 3 times, and then sterilized with 0.1% mercuric chloride solution for 10 minutes, rinsed with sterile water for 4 times, sucked out the water with sterile filter paper, and then inoculated into the induction medium for bud induction culture. After inoculation, first cultivate in total darkness at 25°C for 7 days, then place it under light for 12 hours a day, with a light intensity of 2000lx, and place it at a temperature of 25°C for 25 days to differentiate adventitious buds, and after 45 days Adventitious buds were produced, and the induction rate was 83.62%. The induction medium is: MS+1.0mg / L 6-BA+0.3mg / L NAA+20g / L sucrose+4.0g / L agar, with a pH of 5.5.

[0017] ...

Embodiment 2

[0021] (1) Induction culture: After the collected stem segments are washed with clear water to remove surface attachments, soak in laundry detergent for 8 minutes, then rinse with running water for 3 hours, disinfect with 75% ethanol solution in an ultra-clean workbench for 30 seconds, and then wash with sterile water 5 times, and then sterilized with 0.1% mercuric chloride solution for 15 minutes, rinsed with sterile water for 6 times, sucked out the water with sterile filter paper, and inoculated into the induction medium for bud induction culture. After inoculation, culture in total darkness at 28°C for 10 days, then place in light for 14 hours a day, with a light intensity of 3000lx, and culture at a temperature of 28°C for 21 days to differentiate adventitious buds. Adventitious buds were produced, and the induction rate was 87.93%. The induction medium is: MS+3.0mg / L 6-BA+0.5mg / L NAA+25g / L sucrose+4.5g / L agar, with a pH of 5.8.

[0022] (2) Proliferation culture: ...

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Abstract

The invention discloses a swordlike atractylodes rhizome tissue culture and rapid propagation method. By adopting the method, various problems such as varietal complexity caused by artificial cultivation and variety degeneration, serious diseases and yield reduction caused by long-time vegetative propagation can be solved. Plant tissue culture has the characteristics of rapid propagation, low cost and high multiplication coefficient; and according to the swordlike atractylodes rhizome tissue culture and rapid propagation method disclosed by the invention, stems with buds are taken as explants, a swordlike atractylodes rhizome tissue culture and rapid propagation system can be established by virtue of the steps such as induction, multiplication, rooting and acclimatization and transplanting, so that the propagation speed and scale of seedlings can be expanded, new swordlike atractylodes rhizome varieties with high yield and high quality can also be bred on the basis of expanding the propagation speed and scale of the seedlings, and the economic benefits of swordlike atractylodes rhizome planting can be increased; and the swordlike atractylodes rhizome tissue culture and rapid propagation method has important significance in solving the realistic problem of short supply of swordlike atractylodes rhizome and protecting wild swordlike atractylodes rhizome resources.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for rapid propagation of herb tissue culture. Background technique [0002] Cangzhu ( Atractylodes lancea ) belongs to the Atractylodes genus of the Compositae family, and is a perennial, perennial herb. Cangzhu is mainly distributed in Jiangsu, Hubei, Anhui, Zhejiang, Jiangxi, Henan and other places. Cangzhu is mainly used to treat symptoms such as dampness and spleen and stomach, loss of appetite, indigestion, cold-damp vomiting and diarrhea, fullness of the stomach and abdomen, and edema. After the "SARS" in 2003, the traditional Chinese medicinal herb Atractylodes atractylodis has gradually been valued by pharmaceutical companies, drug markets, and drug dealers. At present, the demand for Atractylodes atractylodis in the domestic market mainly depends on wild Atractylodes atractylodes, but the resources of wild Atractylodes atra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 罗焕荣
Owner 罗焕荣
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