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Method for simultaneously determining contents of plurality of types of water-soluble vitamins in foods or health products

A technology for water-soluble vitamins and health products, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of fewer types of tests, and achieve the effect of improving separation efficiency

Inactive Publication Date: 2015-06-24
SUZHOU YUANZE BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] High-performance liquid chromatography has become the most commonly used analytical method due to its rapid, sensitive and accurate determination, and its ability to be used in conjunction with other analytical techniques. method, although it can simultaneously determine vitamin B 1 , B 2 , B 3 , B 6 Four vitamins, but still fewer types tested

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  • Method for simultaneously determining contents of plurality of types of water-soluble vitamins in foods or health products
  • Method for simultaneously determining contents of plurality of types of water-soluble vitamins in foods or health products
  • Method for simultaneously determining contents of plurality of types of water-soluble vitamins in foods or health products

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Embodiment Construction

[0027] The present invention will be further described below in conjunction with embodiment:

[0028] (1) Experimental equipment

[0029] Agilent 1100Series high performance liquid chromatography (with G1312 binary gradient pump, autosampler, G1314A self-ultraviolet detector, column thermostat, etc.); chromatographic column: Phenomenex Luna C18 (2) (4.6*250mm; 5.0μm); Balance (HANGPING, Model FA2104N); ultrasonic cleaner (SHUMEI, Model KQ5200E); pulverizer (XA-1A); centrifuge (Anke TDL-40B); 1ml syringe; pipette.

[0030] (2) Reagents and standards required for the experiment

[0031] Standard products: ascorbic acid (VC, purity 99.9%), thiamine hydrochloride (VB 1 , purity 99.0%), riboflavin (VB 2 , purity 99.0%), nicotinamide (VB 3 , purity 99.9%), D-pantothenic acid hemicalcium salt (VB 5 , purity 99.0%), pyridoxamine (VB 6 , purity 99.9%), folic acid (VB 9 , purity 98.0%), cyanocobalamin (VB 12 , purity 99.0%). Both were purchased from Sigma Company.

[0032] Re...

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Abstract

The invention discloses a method for simultaneously determining the contents of a plurality of types of water-soluble vitamins in foods or health products. The method comprises the following specific steps: preparing a sample mixed standard solution and a solution to be detected; carrying out gradient elution and separation on all the vitamins by using a high performance liquid chromatograph by taking an acetonitrile-phosphoric acid solution as a mobile phase; detecting all mixed standard samples and the solution to be detected; carrying out linear regression according to detection data of the plurality of groups of mixed standard samples; and analyzing and calculating the contents of the water-soluble vitamins including VB1, VB2, VB3, VC, VB5, VB6, VB9 and VB12 in the solution to be detected according to linear regression equations and the detection data of the solution to be detected, so as to deduce the contents of all the water-soluble vitamins in the detected foods or health product samples. According to the method disclosed by the invention, the detection analysis of the eight types of water-soluble vitamins can be finished by one step, the separation efficiency of the plurality of types of vitamins can be effectively improved, and the samples with relatively complicated base materials can be determined.

Description

technical field [0001] The invention relates to a method capable of simultaneously determining the contents of multiple water-soluble vitamins in food or health care products. Background technique [0002] Vitamins are low-molecular organic compounds that are indispensable for maintaining normal substance metabolism and certain special physiological functions of the human body, and most of them are components of prosthetic groups and enzymes. Vitamins can be divided into fat-soluble vitamins and water-soluble vitamins according to their physical properties. Water-soluble vitamins including B vitamins (VB 1 , VB 2 , VB 3 , VB 6 , VB 12 etc.), VC, pantothenic acid, etc. The human body needs to take in different vitamins every day to participate in the normal physiological metabolic process of the human body, and these vitamins mainly come from food. However, too much or too little vitamin intake by the human body may cause physical discomfort, and in severe cases, it ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 张盟李鹏高张先勇
Owner SUZHOU YUANZE BIOTECH
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