Preparation method of a microsphere stacked shape memory porous scaffold

A porous scaffold and memory technology, which is applied in the field of minimally invasive implant surgery, can solve the problems of uneven pore structure and low drug loading efficiency of the scaffold, and achieve the effects of good adhesion, uniform size and good biocompatibility

Inactive Publication Date: 2017-04-12
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese Patent Application No. 201210275835.9 discloses a method for preparing a porous scaffold with shape memory function, but this method uses sucrose as a porogen, which easily leads to uneven pore structure of the scaffold itself and low drug loading efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0019] 1) Preparation of drug-loaded calcium alginate hydrogel microspheres:

[0020] Add 20ug of bone morphogenetic protein BMP-2 in 30ml mass fraction of 0.5% sodium alginate solution, then push it into 30ml mass fraction of 2% calcium chloride solution by micro-flow pump electrospray technology, so that A cross-linking reaction occurred to generate calcium alginate hydrogel microspheres loaded with BMP-2, the size of the microspheres was 334 μm, and the prepared calcium alginate hydrogel microspheres were stored in a refrigerator at -20°C. In order to avoid inactivation of drug factors. During the preparation process, the pushing speed of the microflow pump was 60mL / h, and the voltage of the high-voltage direct current was 20kv.

[0021] 2) Preparation of porous structure:

[0022] The shape memory scaffold material, 4,4`-diphenylmethane diisocyanate (MDI) coupled polyethylene glycol-polycaprolactone copolymer (PEG-PCL-PU), was dissolved in DMF and CH 2 Cl 2 In the mixe...

Embodiment 1

[0024] Weigh 5 grams of solid sodium alginate powder and 20 grams of solid calcium chloride powder and add them into large beakers each containing 1000 ml of distilled water, and magnetically stir for two hours to fully dissolve them. Then use a syringe to take 30ml of sodium alginate solution mixed with 10ug of BMP-2 and fix the syringe on the microflow pump. Under the condition of high-voltage direct current of 20KV, the sodium alginate solution is added dropwise to Petri dish containing calcium chloride solution. The microspheres in the petri dish are collected to obtain the calcium alginate hydrogel microspheres loaded with BMP-2.

[0025] Dissolving shape memory scaffold material PEG-PCL-PU in DMF and CH 2 Cl 2(v / v is 2:8) mixed solution, according to the ratio of material to microsphere mass ratio of 1:4, the solution is poured into the mold with calcium alginate hydrogel microspheres stacked, ultrasonically dispersed and placed in In a fume hood, after the solvent ev...

Embodiment 2

[0027] This example is basically the same as Example 1, the difference is: when preparing calcium alginate hydrogel microspheres, three kinds of sodium alginate solutions with different mass fractions of 1.5%, 1% and 0.25% were used, and the prepared seaweed The sizes of calcium acid hydrogel microspheres were 588 μm, 425 μm, and 216 μm, respectively.

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PUM

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Abstract

The invention discloses a method for preparing a microsphere-stacked shape memory porous scaffold, in which a drug-carrying and porous structure is formed through the action of a porogen and a drug in a material with a shape memory function to prepare a shape memory function and a drug function The porous scaffold material loaded with drug factors is prepared through 1) preparation of drug-loaded calcium alginate hydrogel microspheres and 2) preparation of porous structure. The prepared porous scaffold has good shape memory, biocompatibility and biodegradability, and also has drug sustained release function, and will have great application prospects in tissue engineering. Tests have proved that the preparation method of the present invention has the advantages of controllable and uniform pore size, no need to sacrifice porogenous materials, and low-cost and easy-to-obtain raw materials.

Description

technical field [0001] The present invention relates to biomaterials and functional polymer materials, in particular to a method for preparing a microsphere-stacked drug-loaded shape-memory porous scaffold, which combines the shape-memory function with tissue engineering and drug-controlled release functions, and is mainly used in microspheres. invasive implant surgery, the field of biomaterials that promote bone tissue growth. Background technique [0002] Shape memory polymers (SMPs) are a new class of functional polymer materials. SMPs are polymers that can change shape in response to environmental changes under appropriate stimulus conditions (such as temperature, light, electromagnetic or solvent, etc.). Shape memory polyurethane generally consists of a soft segment and a hard segment. The soft segment is mainly composed of polyester polyol or polyether polyol, and the hard segment usually includes diisocyanate and chain extender. Therefore, the transition temperature...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/54A61L27/20A61L27/56A61L27/50A61L27/18
Inventor 周绍兵狄淑斌刘典卢柳璇杨光龚涛刘显
Owner SOUTHWEST JIAOTONG UNIV
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