Preparation process of animal placenta extract

A preparation process, animal placenta technology, applied in the field of animal placenta extract preparation process, can solve the problems of low extraction efficiency, lack of extract refining process, high fat content, etc., to improve purity and quality, improve extraction efficiency, and improve activity Effect

Active Publication Date: 2015-07-01
ZHEJIANG HUAERCHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Such as the above method and the products prepared therefrom have the following disadvantages: (1) the obtained placenta extract is mainly composed of macromolecular proteins, and the absorption efficiency of the human body is low; (2) lack of extraction refining process, the product components are complicated and of low quality; (3) ) The product has a high fat content, which is prone to fat oxidation and shortens the shelf life; (4) The extraction method is simple and extensive, the extraction efficiency is low, and the utilization rate of raw materials is low

Method used

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  • Preparation process of animal placenta extract
  • Preparation process of animal placenta extract
  • Preparation process of animal placenta extract

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preparation example Construction

[0026] The invention discloses a preparation process of an animal placenta extract, and those skilled in the art can refer to the content of this article and appropriately improve the process parameters to realize it. In particular, it should be pointed out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and the relevant personnel can obviously make changes or appropriate changes and combinations to the method and application described herein without departing from the content, spirit and scope of the present invention to realize and Apply the technology of the present invention.

[0027] The animal placenta and reagents used in the preparation process of the animal placenta extract provided by the present invention can be purchased from the market. Among the animal ...

Embodiment 1

[0030] Freshly frozen porcine placenta was taken, fascia and umbilical cord were removed and rinsed. After milling with a colloid mill, add 1:1 water and adjust the pH to 1.8.

[0031] 0.4%, 0.8%, and 1.2% of pig placenta mass were added with high-activity pepsin (enzyme activity not less than 10,000 IU / g), and reacted at 40° C. for 6 hours. During the reaction, stir occasionally and keep the pH at 1.8. After the reaction, heat to 100°C for denaturation for 15 minutes to terminate the enzymatic hydrolysis reaction. After denaturation, pass through a 300-mesh non-woven fabric to filter, and collect the filtrate.

[0032] Adjust the pH of the filtrate to 7.0, add 0.04%, 0.08%, and 0.12% of the weight of pig placenta respectively to trypsin (enzyme activity not less than 3800IU / g), and react at 40°C for 4h. During the reaction, stir occasionally and keep the pH at 7.0. After the reaction, denature at 100°C for 15 minutes to terminate the enzymatic hydrolysis reaction. After ...

Embodiment 2

[0047] Take 6 kg of freshly frozen pig placenta, remove the fascia and umbilical cord, and rinse. After milling with a colloid mill, add 1:1 water and adjust the pH to 1.8.

[0048] Add 0.8% high-activity pepsin by weight of pig placenta, and react at 40° C. for 6 hours. During the reaction, stir occasionally and keep the pH at 1.8. After the reaction, heat to 100°C for denaturation for 15 minutes to terminate the enzymatic hydrolysis reaction. After denaturation, pass through a 300-mesh non-woven fabric to filter, and collect the filtrate.

[0049] Adjust the pH of the above filtrate to 7.0, add 0.08% of the weight of pig placenta trypsin, and react at 40° C. for 4 hours. During the reaction, stir occasionally and keep the pH at 7.0. After the reaction, denature at 100°C for 15 minutes to terminate the enzymatic hydrolysis reaction. After denaturation, cool to room temperature for ultrafiltration to obtain ultrafiltrate. The ultrafiltrate is a light yellow clear solutio...

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Abstract

The invention relates to the field of biotechnology, and especially relates to a preparation method of an animal placenta extract. According to the invention, animal placentas are pretreated, and are respectively mixed with pepsin and trypsin for enzymolysis. Extraction is carried out with an enzymolysis method, such that product purity and quality are greatly improved. With other direct hydrolysis methods, with the method provided by the invention, the contents of obtained placenta powder polypeptide and free amino acids are high, and fat content is very low. During an impurity-removing process, besides ultrafiltration, agarose gel hydrogen bond adsorption chromatography is adopted, such that high-molecular impurities are removed, and product purity is further improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation process of animal placenta extract. Background technique [0002] Animal placenta is a by-product of livestock breeding, especially the reproduction of female animals. In recent years, the research on animal placenta has been increasing, and the physiological activities of many animal placenta, such as sheep, deer, yak, and dairy cows, have been continuously discovered. [0003] Placenta extract is a kind of composite active ingredients derived from animal embryos and placenta. It is rich in amino acids, proteins, hormones, lecithin, polysaccharides, vitamins, mineral elements, etc., and its essence is nucleic acid. It is an ideal Immunomodulators and nutrition, beauty and health care products have the functions of enhancing memory, anti-stress, regulating endocrine, enhancing immunity, preventing and treating senile dementia, delaying aging, nourishing blood and beaut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 贾代宏黄嘉华
Owner ZHEJIANG HUAERCHENG PHARMA
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