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Bionic device for preferably selecting human sperms and carrying out in-vitro fertilization

An in vitro fertilization and sperm technology, applied in biochemical cleaning devices, enzymology/microbiology devices, bioreactors/fermenters for specific purposes, etc., can solve the problem of no sperm selection, and achieve sperm selection and fertilization. Effect

Inactive Publication Date: 2015-07-08
ZHEJIANG CELLPRO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fertilization also needs to be observed under a microscope to put high-quality sperm into the fertilization pool to fertilize the eggs. Therefore, the traditional sperm selection and fertilization process are separated, and there is no integrated device for sperm selection and in vitro fertilization.

Method used

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  • Bionic device for preferably selecting human sperms and carrying out in-vitro fertilization
  • Bionic device for preferably selecting human sperms and carrying out in-vitro fertilization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Such as figure 1 , figure 2 A bionic device for human sperm optimization and in vitro fertilization is shown, including a fertilization pool 1, a bionic uterus 2, a seminal tube 3 and eight bionic fallopian tubes 4, the bionic uterus 2 is set under the fertilization pool 1, and the seminal tube 3 Pass through the fertilization pool and connect with the bionic uterus 1, the lower end of the bionic fallopian tube 4 is connected with the top of the bionic uterus 2, the upper end of the bionic fallopian tube 4 is connected with the outer edge of the fertilization pool 1, and the outer edge of the upper opening of the bionic fallopian tube 4 is higher than the fertilization pool The outer edge of 1, the bionic fallopian tube 4 is provided with a bracket 5, the fertilization pool 1 is provided with a cover 6, the cover 6 is provided with an egg adding tube 7 and an upstream liquid adding tube 8, the semen tube 3 passes through the cover 6, and the bionic uterus 2 The bottom...

Embodiment 2

[0015] It is basically the same as Example 1, except that there are no egg feeding tubes and upstream liquid feeding tubes on the cover, and the semen tubes do not pass through the cover, the bottom of the bionic uterus is pointed, and the bionic fallopian tubes are 2 or 6 or 10 Articles, etc., are not listed here.

Embodiment 3

[0017] Using the bionic device of Example 1, during fertilization, preheat the upstream liquid at 37 degrees [upstream liquid formula: 0.1mol / L NaCl (sodium chloride), 0.4mmol / L KH2PO4 (potassium dihydrogen phosphate), 2.7mmol / L C 6 h 6 o 6 (glucose), 4.7mmol / L KCl (potassium chloride), 0.2mmol / L MgSO4·7H2O (magnesium sulfate heptahydrate), 2mmol / L CaCl2·2H2O (calcium chloride dihydrate), 0.02mol / L Hepes-Na (N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid disodium salt), 4mmol / L NaHCO3 (sodium bicarbonate), 0.3mmol / L C3H3O3Na (sodium pyruvate), 0.34% (v / v ) C3H5O3Na (sodium lactate), 0.002% Phenol Red (phenol red)]. After the semen is liquefied or thawed, the semen is added from the semen tube, and then the upstream fluid is first added from the semen tube, paying attention to make the upstream fluid fill the pipes of each bionic oviduct; then the egg or cumulus complex is added to the fertilization pool through the egg addition tube, Then the upstream liquid is added to...

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PUM

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Abstract

The invention discloses a bionic device for preferably selecting human sperms and carrying out in-vitro fertilization. The bionic device comprises a fertilization tank and further comprises a bionic uterus, a sperm tube and at least two bionic fallopian tubes, wherein the bionic uterus is arranged below the fertilization tank; the sperm tube passes through the fertilization tank and is connected with the bionic uterus; the lower ends of the bionic fallopian tubes are connected with the upper part of the bionic uterus; the upper ends of the bionic fallopian tubes are connected with the outer edge of the fertilization tank; the outer edge of an opening at the upper ends of the bionic fallopian tubes is higher than the outer edge of the fertilization tank; a bracket is arranged outside the bionic fallopian tubes. By adopting the bionic device, an ovum is in the fertilization tank; sperm arrives at the bionic uterus through the sperm tube; then, a high-quality sperm is automatically screened; the high-quality sperm upwardly moves from the bionic uterus and enters the fertilization tank from the opening at the upper ends of the bionic fallopian tubes through the bionic fallopian tubes; the high-quality sperm and the ovum are fertilized; preferable selection of the sperms and in-vitro fertilization are integrated; furthermore, the sperms are simple, convenient and rapid to preferably select and fertilize; and the bionic device is an integrated device for preferably selecting sperms and carrying out in-vitro fertilization.

Description

technical field [0001] The invention relates to a human artificial fertilization device, in particular to a bionic device for human sperm optimization and in vitro fertilization. Background technique [0002] In the physiological and natural fertilization process inside the human body, the sperm are screened by the cervix, uterus and fallopian tube and lured by egg chemoattractants, so that the best quality sperm reach the ampulla at the end of the fallopian tube, find the egg and fertilize it. Human infertility treatment requires the use of assisted reproductive technology, including artificial insemination, in vitro fertilization and other treatment methods. In vitro fertilization not only needs to obtain high-quality sperm, but also mixes and fertilizes with eggs or cumulus complexes in vitro. In vitro sperm selection and fertilization depend on sperm selection technology and fertilization system, which is directly related to the success or failure of the entire assisted...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00
Inventor 曾桥薛志刚薛金锋
Owner ZHEJIANG CELLPRO BIOTECH
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