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Method of non-chelating double-cloud-point extraction of lead in trace amount from environmental water samples and foods

A double cloud point, non-chelating technology, applied in the preparation of test samples, etc., can solve the problems of reducing the enrichment factor of the experimental method, reducing the accuracy of the analysis method, and affecting the analysis and measurement, so as to save materials and reagents, and operate Simple and easy to implement, low experimental cost effect

Inactive Publication Date: 2015-07-08
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method still has many defects, such as high concentration of surfactant may block the analytical column, affect the analysis and measurement or even damage the instrument, and diluting the surfactant will reduce the enrichment factor of the experimental method
In addition, the extraction of other interfering substances in the actual sample into the surfactant will also interfere with the determination and reduce the accuracy of the analytical method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for non-chelating double cloud point extraction of trace lead in environmental water samples and food, using environmental water samples and food (Yudai River water, fish, spinach) as raw materials, comprising the following steps:

[0026] (1) In three 10mL glass centrifuge tubes, add 9mL containing spiked lead ions (70μg L -1 )

[0027] Environmental water samples and food (Yudai River water, fish, spinach) solution and 1mL temperature-sensitive triblock copolymer [(PEO) 10 (PPO) 23 (PEO) 10 ] (L44 15%) solution, adjust the pH of the mixed solution to 9.0 with an alkaline buffer solution;

[0028] (2) Place the centrifuge tube in a constant temperature water bath at 60°C for 10 minutes, then centrifuge at 3000 rpm for 5 minutes

[0029] from operation;

[0030] (3) After separation, use a straw to suck out the upper phase aqueous solution, and inject 0.3mL pH solution into the lower phase L44 rich phase

[0031] For 2.5 acid buffer solution.

[0032] (4...

Embodiment 2

[0036] A method for non-chelating double cloud point extraction of trace lead in environmental water samples and food, using environmental water samples and food (Yudai River water, fish, spinach) as raw materials, comprising the following steps:

[0037] (1) In three 10mL glass centrifuge tubes, add 9mL containing spiked lead ions (70μg L -1 )

[0038] Environmental water samples and food (Yudai River water, fish, spinach) solution and 1mL temperature-sensitive triblock copolymer [(PEO) 10 (PPO) 23 (PEO) 10 ] (L44 20%) solution, adjust the pH of the mixed solution to 9.5 with an alkaline buffer solution;

[0039] (2) Place the centrifuge tube in a water bath at a constant temperature of 70°C for 10 minutes, then centrifuge at 3000 rpm for 5 minutes.

[0040] from operation;

[0041] (3) After the separation, use a pipette to suck out the aqueous solution of the upper phase, and inject 0.3 mL of an acidic buffer solution with a pH of 3.5 into the lower phase L44-enriched ...

Embodiment 3

[0046] A method for non-chelating double cloud point extraction of trace lead in environmental water samples and food, using food and environmental water samples (fish, spinach, Yudai River water) as raw materials, comprising the following steps:

[0047] (1) In three 10mL glass centrifuge tubes, add 9mL containing spiked lead ions (70μg L -1 )

[0048] Environmental water samples and food (Yudai River water, fish, spinach) solution and 1mL temperature-sensitive triblock copolymer [(PEO) 10 (PPO) 23 (PEO) 10 ] (L44 25%) solution, adjust the pH of the mixed solution to 10.0 with an alkaline buffer solution;

[0049] (2) Place the centrifuge tube in a water bath at a constant temperature of 75°C for 10 minutes, and then perform centrifugation at 3000 rpm for 5 minutes.

[0050] from operation;

[0051] (3) After the separation, suck out the aqueous solution of the upper phase with a pipette, and inject 0.3 mL of an acidic buffer solution with a pH of 4.5 into the lower phase ...

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PUM

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Abstract

The invention discloses a method of non-chelating double-cloud-point extraction of lead in trace amount from environmental water samples and foods and belongs to the technical field of metal ion extraction separation. In the method, a temperature-sensitive triple-block copolymer [(PEO)10(PPO)23(PEO)10](L44), as an nonionic surfactant, is applied in the double-cloud-point extraction, wherein the polyoxyethylene-polyoxypropylene-polyoxyethylene (PEO-PPO-PEO) temperature-sensitive triple-block copolymer contains a hydrophilic PEO unit and a hydrophobic PPO unit. When being heated to a certain temperature, the PEO-PPO-PEO solution can form a two-phase system. The method is used for separating and enriching lead in trace amount from the environmental water samples and foods, wherein the marked extraction rate is 89.45-99.19%, the enriching factor is 30 and the detection limit is 1.9 [mu]g / L. Meanwhile, the copolymer can be recycled and reused for two times, wherein the extraction rates in the two times are both not less than 90%.

Description

technical field [0001] The invention relates to a method for non-chelating double cloud point extraction, separation and enrichment of trace lead in environmental water samples and food (Yudai River water, fish, spinach), which belongs to the technical field of metal ion extraction and separation. Background technique [0002] With the rapid development of modern industry and agriculture, the discharge of industrial wastewater, waste residue and waste gas has become the main pollution source of heavy metal ions in water, soil and atmosphere. Among them, lead ions can accumulate in the human body through a complex food chain. When the concentration is too high, it will cause damage to the nervous system, digestive system, hematopoietic system and kidney function, and even affect mental development. Therefore, it is particularly important to detect trace lead content in food and environmental water samples. Because the content of lead ions in the actual sample is very low a...

Claims

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Application Information

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IPC IPC(8): G01N1/40G01N1/34
Inventor 韩娟刘莹莹王赟王蕾陈桐倪良
Owner JIANGSU UNIV