Targeted mesoporous molecular imaging probe and preparation method thereof

Abstract

The invention relates to a targeted mesoporous molecular imaging probe and a preparation method thereof. The targeted mesoporous molecular imaging probe comprises two parts including a surface plasma resonance core layer and a peripheral coating layer, wherein the surface plasma resonance core layer at least comprises a gold nanorod I, a gold nanorod II and substrate nano particles; the peripheral coating layer is formed by fluorescent molecules, sulfydryl small biological molecules and polystyrene sulfonate connectors; the gold nanorod I and the gold nanorod II are cohered on the substrate nano particles, so that the surface plasma resonance core layer can be formed; the distance between the gold nanorod I and the gold nanorod II meets the condition that d is more than or equal to (r1-r2) / 2 and less than or equal to (r1+r2) / 2; the exterior of the core layer is combined with the sulfydryl small biological molecules by a gold sulfhydryl bond, the fluorescent molecules are combined with the sulfydryl small biological molecules by an amide covalent bond, and polystyrene sulfonate is directly connected on a substrate in a bridging way. The invention also provides the preparation method of the targeted mesoporous molecular imaging probe. Blue shift or red shift is generated by surface excitation, and the characteristics of luminescence of a fluorescent structure are matched, so that the brightness is increased by 8-10 orders of magnitude. The targeted mesoporous molecular imaging probe has very high fluorescence characteristic, thus being applied to the diagnosis of tumor fluorescence imaging; the targeted mesoporous molecular imaging probe is especially suitable for detection and diagnosis of tumors in the dark field at the deep part of tissue cells.

Description

technical field [0001] The invention relates to a targeting mesoporous molecular imaging probe and a preparation method thereof, in particular to a targeting mesoporous molecular imaging probe based on surface plasmon resonance gold nanorods and a preparation method thereof. Background technique [0002] Visible light imaging has many advantages over nuclear magnetic resonance imaging, such as easy operation, intuitive results, fast measurement, no radiation to the human body, low environmental requirements, and low cost. Therefore, the application of visible light imaging technology to clinical medical diagnosis has become the current An important branch of medical imaging. [0003] At present, one of the main reasons limiting the clinical application of visible light imaging technology is the lack of suitable fluorescent probes for human body. At the same time, high contrast is also the main technical bottleneck that plagues fluorescent probes. For example, the prior art ...

AI Technical Summary

Problems solved by technology

Although the above-mentioned fluorescent probes based on gold nanomaterials in the prior art have solved the problems of safety and stability well, they all have a single luminescence wavelength of nanocrystals, which cannot be adjusted to suit the luminescence wavelength of fluorescent tissues, and provide high Problems with Brightness Fluorescence Imaging

Images