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New preparation method of fluorescent carbon nanoparticles and application in biological samples

A carbon nanoparticle and new method technology, applied in nanotechnology, fluorescence/phosphorescence, chemical instruments and methods, etc., can solve the problems of cumbersome operation, low yield, unstable fluorescence emission wavelength, etc., and achieve broad application prospects and cost. Low, the effect of improving processing efficiency

Inactive Publication Date: 2015-07-22
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The preparation of carbon quantum dots by the above method often requires high temperature heating, or requires conditions such as voltage and strong acid, and the operation is relatively cumbersome, the yield is low, and carbon quantum dots cannot be prepared in large quantities; change, fluorescence emission wavelength instability

Method used

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  • New preparation method of fluorescent carbon nanoparticles and application in biological samples
  • New preparation method of fluorescent carbon nanoparticles and application in biological samples
  • New preparation method of fluorescent carbon nanoparticles and application in biological samples

Examples

Experimental program
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Effect test

example 1

[0024] The preparation of fluorescent carbon nanoparticles of the present invention is as follows:

[0025] (1) Preparation of sugar solution: Weigh 0.5 g of glucose and add it to 2 mL of deionized water, and mix it ultrasonically for 10 minutes to form a uniform and transparent sugar solution;

[0026] (2) Preparation of fluorescent carbon nanoparticles: the sugar solution obtained in step (1) is added to a container containing 2.5 grams of P 2 o 5 In a small beaker, the mixture reacted rapidly, spontaneously exothermic to generate a large amount of foam, the volume of the solution expanded, and the color turned brownish black, and the mixture gradually cooled to room temperature after 10 minutes;

[0027] (3) Purification of fluorescent carbon nanoparticles: Pour the brown-black mixture obtained in step (2) into an appropriate amount of water, stir and mix, ultrasonicate for 10 minutes, then centrifuge the mixture at 13000rmp for 30 minutes, collect the supernatant, and pla...

example 2

[0029] (1) Preparation of sugar solution: Weigh 0.5 g of sucrose and add it to 2 mL of deionized water, and mix it ultrasonically for 10 minutes to form a uniform and transparent sugar solution;

[0030] (2) Preparation of fluorescent carbon nanoparticles: heat the sugar solution obtained in step (1) to 80°C, add 2 o 3 In a small beaker, the mixture reacted rapidly, spontaneously exothermic to generate a large amount of foam, the volume of the solution expanded, and the color turned brownish black, and the mixture gradually cooled to room temperature after 10 minutes;

[0031] (3) Purification of fluorescent carbon nanoparticles: Pour the brown-black mixture obtained in step (2) into an appropriate amount of water, stir and mix, ultrasonicate for 10 minutes, then centrifuge the mixture at 13000rmp for 30 minutes, collect the supernatant, and place it in a dialysis bag for use. Ionized water was dialyzed to remove residual sugar and ions. Finally, the fluorescent carbon nanopa...

example 3

[0035] Fluorescent carbon nanoparticles for the detection of quercetin in urine

[0036] 1.5 mL of urine from healthy volunteers was spiked with quercetin standard solution, and then diluted to 100 μg / ml. Add 330 μL of fluorescent carbon nanoparticles into a 5 mL volumetric flask, add 100 μL of spiked urine, dilute to the mark with 0.01 M phosphate buffer solution, pH 6.0, mix and sonicate. After ultrasonication for 22 min, a fluorescence spectrophotometer was used for spectral analysis, the excitation wavelength was 370 nm, the excitation and emission slit widths were both 5 nm, and the fluorescence emission spectrum was scanned in the range of 300-700 nm. Determine the concentration of quercetin in the sample solution, and calculate the amount of quercetin in the solution. Correspondingly, a control group experiment without spike in urine was also determined together. Experiments show that carbon nanoparticles have good dispersion performance in urine of biological samples...

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Abstract

The invention relates to preparation of novel fluorescent carbon nanoparticles and an application in biological samples and belongs to the technical field of pharmaceutical analysis. By a phosphorus pentoxide dehydration method, a carbohydrate compound is used as a carbon source and reactants are simply mixed to prepare carbon nanoparticles with fluorescent property by a one-step method. The preparation process is a process of a spontaneous reaction. Without external heating or complex operations, a lot of carbon nanoparticles can be rapidly produced. The prepared carbon nanoparticles are characterized in that emission wavelength is fixed and will not change with excitation wavelength. In a phosphate buffered solution, fluorescence intensity of the carbon nanoparticles is obviously reduced with increasing of concentration of quercetin, and influence of other coexisting substances on fluorescence intensity of the carbon nanoparticles is little. Under optimized conditions, a good linear relationship between fluorescence intensity of the carbon nanoparticles and the concentration of quercetin is shown. The preparation method is pollution-free, simple, reliable and low-cost, and the prepared fluorescent carbon nanoparticles have a wide application prospect in aspects of detection of traces of pharmaceuticals in complex biological samples and detection and control of heavy metal in the environment.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, in particular to the preparation of novel fluorescent carbon nanometer particles, which are directly applied to the analysis of trace drugs in complex biological samples. Background technique [0002] Quercetin is a typical polyhydroxy flavonoid that is abundant in flowers, leaves, bark and fruits of many plants. Over the past few years, quercetin has been known for its many potentially beneficial effects on human health, including cardiovascular protection, anticancer activity, antiulcer effects, antiallergic activity, antibacterial, antiviral activity, and anti-inflammatory effects. Su has aroused great interest. In clinical treatment, quercetin, etc. have relatively large side effects while exerting therapeutic effects, so blood drug concentration detection or urine drug concentration detection is required. However, due to the low concentration of drugs in biological samples, and the p...

Claims

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Application Information

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IPC IPC(8): C01B31/02C09K11/65B82Y40/00G01N21/64
Inventor 何华肖得力左朋礼袁丹华潘仁锋
Owner CHINA PHARM UNIV
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