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Freshwater microalga and application thereof

A technology of microalgae and fresh water, which is applied in the direction of application, microbes, microbes, etc., to achieve the effects of excellent oil content, excellent culture cycle, and huge application potential

Active Publication Date: 2015-07-29
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, there is currently no mature and reliable method for controlling the damage of rotifers

Method used

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  • Freshwater microalga and application thereof
  • Freshwater microalga and application thereof
  • Freshwater microalga and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Isolation of Didymogenes sp.HN-4 strain

[0034] Didymogenes sp.HN-4 was isolated from a pond in a certain place in Hunan Province. The specific process is as follows: take an appropriate amount of pond water samples with a sterile bottle, and under aseptic conditions in the laboratory, spread the BG-11 medium plate and place it in a light incubator. Cultivate at 25°C until algal colonies appear, pick a plurality of algal colonies and perform multiple streaking and separation steps until completely purified algal colonies are obtained. That is, HN-4 was obtained, which was identified as a species of the genus Didymogenes through the sequencing analysis of 18S rDNA and ITS1-5.8S-ITS DNA sequences.

[0035] Pick a single colony of Didymogenes sp.HN-4 on a solid plate under sterile conditions, and obtain purified algal colonies by repeatedly streaking and picking. It was deposited in the General Microbiology Center of China Microbiological Culture Collection Ma...

Embodiment 2

[0036] Embodiment 2: Initial cultivation of Didymogenes sp.HN-4 algae liquid

[0037] Under laboratory conditions, the above-mentioned Didymogenes sp.HN-4 was preserved as a test tube slant culture medium, or as a liquid culture medium in an Erlenmeyer flask, and the medium used was BG-11 medium. 2 months is a cycle to transfer and preserve the activity of algal species. The algae liquid used for inoculation is generally at 200μmol photons / m 2 OD of fresh algae cultured at 25°C for 7-10 days under light below / s 750 reach 2.0, inoculate to 50ml sterile BG-11 culture medium (NaNO 3 1.5g / L; K 2 HPO 4 40mg / L; MgSO 4 ·7H 2 O75mg / L; CaCl 2 2H 2 O36mg / L; Citric acid 6mg / L; Ferric ammonium citrate 6mg / L; Na 2 EDTA1mg / L; NaCO 3 20mg / L;H 3 BO 3 2.86mg / L; MnCl 2 4H 2 O1.81mg / L; ZnSO 4 ·7H 2 O0.222mg / L; NaMoO 4 2H 2 O0.39mg / L; CuSO 4 ·5H 2 O0.079mg / L; Co(NO 3 ) 2 ·6H 2 O0.049mg / L; deionized water 1L, adjust the pH to 7.4) in a 100ml Erlenmeyer flask; then cultivate...

Embodiment 3

[0038] The expanded cultivation of embodiment 3 Didymogenes sp.HN-4 algae liquid and its use in the production of oil

[0039] Inoculate the initial algae liquid obtained above into 250ml of sterilized BG-11 culture liquid (the composition in the BG-11 culture liquid is K 2 HPO 4 40mg / L; MgSO 4 ·7H 2 O75mg / L; CaCl 2 2H 2 O36mg / L; Citric acid 6mg / L; Ferric ammonium citrate 6mg / L; Na 2 EDTA1mg / L; NaCO 3 20mg / L;H 3 BO 3 2.86mg / L; MnCl 2 4H 2 O1.81mg / L; ZnSO 4 ·7H 2 O0.222mg / L; NaMoO 4 2H 2 O0.39mg / L; CuSO 4 ·5H 2 O0.079mg / L; Co(NO 3 ) 2 ·6H 2 O0.049mg / L; 1L of deionized water, adjust the pH to 7.4), add sodium nitrate to the culture medium at the same time, the content of sodium nitrate is 0.375g / L, and the final concentration of the cells is OD 750 0.2, using a column culture device, continuous light, the light intensity is 400-500μmol photons / m 2 / s, the temperature is 24℃~30℃, CO 2 The concentration was 2%, and after 16 days of aerated culture, it was coll...

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Abstract

The invention relates to a microalga screening method, and concretely relates to a freshwater microalga and an application thereof. A microalga strain is a freshwater microalga strain Didymogenes sp. HN-4, is preserved on Oct. 24, 2013, and has a preservation number of CGMCC No.8401. The microalga strain is used for producing oil, biodiesel, algal proteins, fatty acids or biomasses. The invention also discloses growth conditions and oil contents of the microalga strain under different medium components. The application of the microalga provides an environmentally-friendly cheap technology for producing biodiesel and reducing insect pests of microalgae.

Description

technical field [0001] The invention relates to a method for screening microalgae, in particular to a freshwater microalgae and its application. Background technique [0002] Due to the energy crisis and global warming, coupled with human beings' emphasis on environmental protection issues, and the expectation of future fossil fuel price increases, the search for renewable new energy sources that replace fossil energy has increasingly become a research hotspot in the scientific and industrial circles. Among new energy sources, microalgae diesel is considered to be one of the most promising renewable green energy sources. Microalgae have high photosynthetic efficiency and can make full use of marginal land resources for large-scale farming, avoiding competition with food crops for valuable land resources. In addition, microalgae can be used for sewage treatment and carbon capture. Therefore, the development of microalgae biofuel is also beneficial to environmental protectio...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64C12P21/00A61K36/02A61P33/02C12R1/89
CPCA01N65/00A01N65/03C12P7/6463C12N1/125C12N1/205C12R2001/12C12R2001/89Y02E50/10
Inventor 胡光荣李福利袁程范勇
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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