A method for non-destructive extraction of mineral elements from Lycium barbarum leaves by flash shearing
A non-destructive, mineral element technology, applied in the preparation of test samples, measurement of color/spectral properties, etc., can solve problems such as large environmental pollution, adverse health, low temperature element loss, etc., and achieve short extraction time and low solvent consumption. , the effect of convenient operation
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Embodiment 1
[0038] 1. Wash the wolfberry leaves (Foliis medlar), dry (dry at 60°C for 6 hours), grind and pass through a 60-mesh sieve.
[0039] 2. Accurately weigh 50g of the sieved powder into a beaker, add acetone to make the acetone liquid level 2cm below the wolfberry leaf powder, shake for 20min, filter and dry to obtain the fat-removed wolfberry leaf powder.
[0040] 3. Move the filter cake into a beaker and add 1.5L of deionized water to dissolve it, add cellulase to make the concentration reach 150U / mL, let stand for 2h, then add 100mL of glacial acetic acid solution (the volume of glacial acetic acid and water in the glacial acetic acid solution) The ratio is 1:1).
[0041] 4. Use a flash extractor (motor speed: 6000r / min) to extract for 90s, homogenize and filter with suction, and then extract the residue twice with a flash extractor after suction.
[0042] 5. Combine the extracts obtained three times, concentrate and dry by freeze-drying (110kPa, -50°C, drying for 12h). The content of...
Embodiment 2
[0044] 1. Wash the wolfberry leaves (Foliis medlar), dry (dry at 60°C for 6 hours), grind and pass through a 60-mesh sieve.
[0045] 2. Accurately weigh 50g of the sieved powder into a beaker, add acetone to make the acetone liquid level 2cm below the wolfberry leaf powder, shake for 20min, filter and dry to obtain the fat-removed wolfberry leaf powder.
[0046] 3. Move the filter cake into a beaker and add 1.5L of deionized water to dissolve it, add cellulase to make the concentration reach 150U / mL, let stand for 2h, then add 100mL of glacial acetic acid solution (the volume of glacial acetic acid and water in the glacial acetic acid solution) The ratio is 1:1).
[0047] 4. Use a flash extractor (motor speed: 10000r / min) to extract for 90 seconds, homogenize and filter with suction, and then extract the residue twice with a flash extractor after suction.
[0048] 5. Combine the three extracts, concentrate and dry them by freeze-drying (110kPa, -50°C, drying for 12 hours). The content...
Embodiment 3
[0050] 1. Wash the wolfberry leaves (Foliis medlar), dry (dry at 60°C for 6 hours), grind and pass through a 60-mesh sieve.
[0051] 2. Accurately weigh 50g of the sieved powder into a beaker, add acetone to make the acetone liquid level 2cm below the wolfberry leaf powder, shake for 20min, filter and dry to obtain the fat-removed wolfberry leaf powder.
[0052] 3. Move the filter cake into a beaker and add 2L of deionized water to dissolve it, add cellulase to make the concentration reach 150U / mL, let stand for 2h, then add 100mL of glacial acetic acid solution (volume ratio of glacial acetic acid to water in glacial acetic acid solution 1:1).
[0053] 4. Use a flash extractor (motor speed: 6000r / min) to extract for 60 seconds, homogenize and filter with suction, and then extract the residue twice with a flash extractor after suction.
[0054] 5. Combine the extracts obtained three times, concentrate and dry by freeze-drying (110kPa, -50°C, drying for 12h). The content of calcium is ...
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