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Crystal skeleton specimen manufacturing method

A production method and specimen technology, applied in the field of medicine or biological science, can solve the problems of difficult skeletal development and deformity research and teaching, inability to clearly observe skeletal development, lack of large-scale production of crystal skeleton specimens, etc., to achieve extensive art appreciation and teaching. Use and produce a variety of effects with superior quality

Inactive Publication Date: 2015-09-02
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its appearance is poor, and the development of bones cannot be clearly observed. It is not easy to conduct research and teaching on various bone development deformities. At present, there is no trend of crystal bone art in China, and there is also a lack of large-scale production of crystal bone specimens. factory
Moreover, domestic related technologies are limited, and the technology for making bone specimens that are more difficult still needs to be improved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The method for making chicken embryo crystal bone specimens, the specific steps are as follows:

[0031] Take 12-day-old chicken embryos, fix them in 50% ethanol, and put them on a shaker for 12 hours; then put them in 100% methanol for 12 hours; then put them in 100% acetone for 12 hours; finally put the chicken embryos in 100 Fix in % ethanol for 12 hours;

[0032] Prepare Alcian blue staining solution, put the chicken embryos in the staining solution for about 14 hours after peeling; then put them in 100% ethanol to wash off the floating color, put the chicken embryos in 5% potassium hydroxide solution for about 24-24 hours 28 hours; prepare Alizerin red staining solution, put the chicken embryos into the staining solution and dye for 3 to 10 days (according to the size of the embryo); then put the chicken embryos into 1.5% potassium hydroxide solution for rinsing and non-specific coloring for about 12 hours;

[0033] Put in 50% glycerin for about 48 hours, then ent...

Embodiment 2

[0035] The method for making a turtle crystal bone specimen, the specific steps are as follows:

[0036] Take one or several Brazilian tortoises, fix them in 50% ethanol, and put them on a shaker for 12 hours; then put them in 100% methanol for 12 hours; then put them in 100% acetone for 12 hours; fixed in 100% ethanol for 12 hours;

[0037] Add 250 microliters of 30% hydrogen peroxide to 1000mL of pure water, put it into the Brazilian turtle, and rinse it for 5 days;

[0038] Prepare Alizerin red staining solution, put the Brazilian tortoise in the staining solution and dye for 15-20 days; then put the Brazilian tortoise in 1.5% potassium hydroxide solution for rinsing and non-specific coloring for about 24 hours;

[0039] Put it in 50% glycerin for about 48 hours, and then enter 75% glycerin and 100% glycerin for gradient transparency treatment for about 2 days until the turtle sinks to the bottom of the container; Process finished production.

Embodiment 3

[0041] The method for making fish crystal skeleton specimens, the specific steps are as follows:

[0042] Take one or several fishes, fix them in 50% ethanol, and put them in a shaking table for 12 hours; then put them in 100% methanol for 12 hours; then put them in 100% acetone for 12 hours; Fix in 100% ethanol for 12 hours;

[0043] Add 250 microliters of 30% hydrogen peroxide to 1000mL of pure water, put the small fish in, rinse for 24 hours, rinse for 24 hours;

[0044] Prepare Alizerin red staining solution, put the small fish in the staining solution for 5 days, then put the small fish in 1.5% potassium hydroxide solution to rinse non-specific staining and degrease the surrounding tissues for 3 days;

[0045] Put in 50% glycerin for about 48 hours, then enter 75% glycerin and 100% glycerin for gradient transparency treatment for about 2 days until the fish body sinks to the bottom of the container; Microprocessing completes the fabrication.

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Abstract

The invention discloses a crystal skeleton specimen manufacturing method. The method includes the steps of acquiring an animal specimen and then fixing the animal specimen by 50% ethyl alcohol, 100% methyl alcohol, 100% acetone and 100% ethyl alcohol; degreasing and decolorizing by a 0.5-3% hydrogen dioxide solution and the 100% acetone; performing skeleton dyeing treatment by Alizerin red and / or Alcian blue; removing specificity coloring by the 100% ethyl alcohol and / or 1.5% potassium hydroxide; performing gradient transparentizing treatment by 50% glycerol, 75% glycerol and 100% glycerol; removing soft tissues under a microscope to obtain a crystal skeleton specimen. The crystal skeleton specimen manufacturing method has the advantages that the manufacturing period is shortened to 15-30 days, a manufactured crystal skeleton is excellent in quality, and the method can be used for manufacturing multiple kinds of skeleton specimens and has commercialized mass production potential.

Description

technical field [0001] The invention belongs to the field of medicine or biological science, and relates to a method for preparing an animal specimen, in particular to a method for preparing a crystal bone specimen. Background technique [0002] At present, most of the animal specimens produced in the domestic market are ordinary specimens fixed in formalin. Its appearance is poor, and the development of bones cannot be clearly observed. It is not easy to conduct research and teaching on various bone development deformities. At present, there is no trend of crystal bone art in China, and there is also a lack of large-scale production of crystal bone specimens. factory. Moreover, domestic related technologies are limited, and the technology for making more difficult bone specimens still needs to be improved. Contents of the invention [0003] In order to solve the shortcomings and deficiencies of the prior art, the object of the present invention is to provide a method fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00G09B23/28
Inventor 马征来李忠洋管保章
Owner JINAN UNIVERSITY