A kind of fertilizer making agent and its preparation method and application

A technology of composting and edible fungi, which is applied in the preparation of organic fertilizers, biochemical equipment and methods, applications, etc., can solve the problems of crop growth impact, environmental problems, and damage to the balance of soil flora, so as to achieve excellent fertilizer efficiency and improve fertilizer efficiency. Effect

Active Publication Date: 2018-08-31
BIOGAS SCI RES INST MIN OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, Trichoderma reesei can reproduce in large quantities in the soil, which will have a serious impact on the growth of subsequent crops, and will also destroy the balance of the flora in the soil, causing environmental problems

Method used

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  • A kind of fertilizer making agent and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1) Inoculate Cytophaga fermentans ATCC 19072, Cellulomonas flavigena ATCC 482, Butyrivibrio fibrisolvens ATCC 19171, Clostridium papyrosolvens ATCC 35413 in the cellulose-containing medium at a cell number ratio of 3:4:5:15, seal: seal: static culture , the culture temperature is 30°C, the culture pH is 3, and the culture is 5 days;

[0034] The cellulose-containing culture solution contains 2-7‰CaCO3, 3-7‰NaCl, 5-10‰cellulose, urea 1-4‰, peptone 1-3‰, yeast powder 0.5- 1.5‰, with water as solvent;

[0035] 2) Inoculate Bacillus megaterium ACCC 01742, Candida tropicalis ACCC 20199, and Phaneroderma chrysosporium ACCC30530 in PDA medium at a cell number ratio of 5:15:8, and culture them statically at a temperature of 35°C. Time 3d, in the described PDA culture medium, comprise 20% potato extract liquid by mass percentage, also comprise sucrose 20g / kg;

[0036] 3) Mix the cultures obtained in step 1) and step 2) uniformly at a volume ratio of 1:1, centrifuge, remove the...

Embodiment 2

[0040] 1) Inoculate Cytophaga fermentans ATCC 19072, Cellulomonas flavigena ATCC 482, Butyrivibrio fibrisolvens ATCC 19171, Clostridium papyrosolvens ATCC 35413 in the cellulose-containing medium at a cell number ratio of 17:25:10:28, seal and culture, culture temperature The temperature is 50°C, the culture pH is 10, and the culture time is 5 days;

[0041] The cellulose-containing culture solution contains 2-7‰CaCO3, 3-7‰NaCl, 5-10‰cellulose, urea 1-4‰, peptone 1-3‰, yeast powder 0.5- 1.5‰, with water as solvent;

[0042] 2) Mix and inoculate Bacillus megaterium ACCC 01742, Candida tropicalis ACCC 20199, and Phaneroderma chrysogenum ACCC30530 in PDA medium at a cell number ratio of 14:20:20, and culture them statically at a temperature of 35°C. Time 3d, in the described PDA culture medium, comprise 20% potato extract liquid by mass percentage, also comprise sucrose 20g / kg;

[0043] 3) Mix the cultures obtained in step 1) and step 2) uniformly at a volume ratio of 1:1, centri...

Embodiment 3

[0047] 1) Inoculate Cytophaga fermentans ATCC 19072, Cellulomonas flavigena ATCC 482, Butyrivibrio fibrisolvens ATCC 19171, Clostridium papyrosolvens ATCC 35413 in the cellulose-containing medium at a cell number ratio of 8.5:10:8.5:23. 40°C, culture pH 7, culture time 5 days;

[0048] The cellulose-containing culture solution contains 2-7‰CaCO3, 3-7‰NaCl, 5-10‰cellulose, urea 1-4‰, peptone 1-3‰, yeast powder 0.5- 1.5‰, with water as solvent;

[0049] 2) Inoculate Bacillus megaterium ACCC 01742, Candida tropicalis ACCC 20199, and Phaneroderma chrysosporium ACCC30530 in PDA medium at a cell number ratio of 13:19:18, and culture them statically at a temperature of 35°C. Time 3d, in the described PDA culture medium, comprise 20% potato extract liquid by mass percentage, also comprise sucrose 20g / kg;

[0050] 3) Mix the cultures obtained in step 1) and step 2) uniformly at a volume ratio of 1:1, centrifuge, remove the precipitate, and take the supernatant to obtain the fertilize...

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Abstract

The invention provides a preparation method of a fertilizer making agent. The preparation method includes the following steps that firstly, Cytophaga fermentans ATCC 19072, Cellulomonas flavigena ATCC 482, Butyrivibrio fibrisolvens ATCC 19171 and Clostridium papyrosolvens ATCC 3541 are inoculated to a culture medium with cellulose to be cultured according to the thallus number ratio of 3-17:4-25:5-10:15-28; secondly, bacillus megatherium ACCC 01742, candida tropicalis ACCC 20199 and phanerochaete chrysosporium ACCC 30530 are inoculated to a PDA culture medium to be cultured in a mixed mode according to the thallus number ratio of 5-14:15-20:8-20; thirdly, culture obtained from the first step and the second step is evenly mixed according to the volume ratio of 1:1 and centrifuged, sediment is removed, supernate is taken, and then the fertilizer making agent is obtained. The invention further discloses the fertilizer making agent prepared through the method and application of the fertilizer making agent in preparing edible fungus compost. The fertilizer efficiency of the compost can be remarkably improved, the fertilizer making agent is economical and environmentally friendly, and no influence is generated on growth of crops and soil.

Description

technical field [0001] The invention belongs to the field of edible fungus residue utilization, and in particular relates to a fertilizer making agent and its preparation method and application. technical background [0002] At present, there are few researches on using edible mushroom residues to make fertilizer. The usual research is only to make it a usable fertilizer. The fertilizer efficiency is low, the economic effect is limited, and the environmental pollution is large. [0003] In order to improve fertilizer efficiency, Trichoderma reesei is commonly used in the prior art. However, Trichoderma reesei can reproduce in large quantities in the soil, which will have a serious impact on the growth of subsequent crops, and will also destroy the balance of the flora in the soil, causing environmental problems. [0004] Therefore, how to avoid environmental problems and affect the growth of crops while improving fertilizer efficiency has become an urgent problem to be solv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C05F17/00C12R1/145C12R1/11C12R1/74C12R1/645C12R1/01
CPCY02W30/40
Inventor 张云飞邓宇陈璐贺静郭旭晶朱能敏
Owner BIOGAS SCI RES INST MIN OF AGRI
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