PCA3 mRNA/ACPP mRNA RT-PCR detection primer and detection kit thereof

A detection kit, -CTCCTCAACATGAGAGCTGC-3 technology, applied in the direction of DNA / RNA fragments, recombinant DNA technology, etc., can solve the problems of poor diagnostic sensitivity and specificity of prostate cancer, so as to improve sensitivity and specificity, improve detection sensitivity, and diagnose The effect of high accuracy
CN104878077AInactive Publication Date: 2015-09-02谭巍
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
谭巍
Publication Date
2015-09-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a PCA3 mRNA / ACPP mRNA RT-PCR detection primer and a detection kit thereof. The detection primer comprises an upstream primer represented by 5'-CTCCTCAACATGAGAGCTGC-3' and a downstream primer represented 5'-TATATACTCTCCAAGTTCATA-3'. The detection kit comprises an RT-PCR reaction solution, MMLV RT / Hot start Taq Mix, a positive reference substance and a negative reference substance. The PCA3 mRNA / ACPP mRNA RT-PCR detection kit prepared in the invention is better than PCA3 mRNA / PSA mRNA RT-PCR combination detection kits, and the detection sensitivity of the detection kit prepared in the invention increases to above 90%.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a PCA3mRNA / ACPP mRNA RT-PCR detection primer and a detection kit. Background technique

[0002] ACPP (acid prostatic phosphatase,) encodes a non-specific tyrosine phosphatase that dephosphorylates a variety of substrates under acidic (PH4-6) conditions. ACPP substrates include alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Under the regulation of androgen, prostate epithelial cells synthesize ACPP and secrete it into semen. The expression of ACPP mRNA in the prostate is more than 100 times that of any other tissue. IHC staining with ACPP antibody showed obvious immunohistochemical staining reactions in both intracellular and intercellular in prostate tissue, proving that ACPP is a prostate-specific gene. ACPP protein can be detected in serum. In a pathological state, prostate epithelial cells will be shed and enter the blood and urine. Damaged prost...

Claims

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