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Method for extracting flavonoids from ginkgo biloba leaves with deep eutectic solvent

A technology of flavonoids and low eutectic solvent is applied in the field of flavonoids to achieve the effects of high efficiency, low loss and low energy consumption

Inactive Publication Date: 2015-09-09
TIANJIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to address the above-mentioned problems and provide a method for extracting flavonoids from ginkgo leaves using a deep eutectic solvent. Using the present invention to extract flavonoids can effectively solve the problem of using flammable and explosive organic compounds in the existing flavonoid extraction technology. Problems with solvents

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A method for extracting flavonoids from ginkgo biloba leaves using a eutectic solvent, including the following steps:

[0015] 1) Crush the dried ginkgo leaves, pass through a 10 mesh sieve, take 10 g of ginkgo biloba powder, add 25 ml of choline eutectic solvent and 25 ml of water, and ultrasonically extract at 40°C for 20 minutes, with a power of 100W / cm 2 , The frequency is 20kHz, the extract is centrifuged at 4000rpm for 5 minutes to obtain the flavonoid extract;

[0016] 2) The flavonoids extract obtained in step 1) is filtered through a 0.45 μm filter membrane to remove impurities to obtain a filtered extract.

[0017] The obtained extract was tested by injecting 10 μL with a high performance liquid chromatograph, and the extracted amount of the target substance was calculated to be 160 mg / 10 g.

Embodiment 2

[0019] A method for extracting flavonoids from ginkgo biloba leaves using a eutectic solvent, including the following steps:

[0020] 1) Take 10 grams of Ginkgo biloba powder that has been crushed and passed through a 20-mesh sieve, add 50 ml of choline eutectic solvent and 500 ml of water, and ultrasonically extract at 50°C for 40 minutes, power 10W / cm 2 , The frequency is 20kHz, and the extract is centrifuged at 4000rpm for 5 minutes to obtain the flavonoid extract;

[0021] 2) The flavonoids extract obtained in step 1) is filtered through a 0.45 μm filter membrane to remove impurities to obtain a filtered extract.

[0022] The obtained extract was tested by injecting 10 μL with a high performance liquid chromatograph, and the extracted amount of the target substance was calculated to be 200 mg / 10 g.

Embodiment 3

[0024] A method for extracting flavonoids from ginkgo biloba leaves using a eutectic solvent, including the following steps:

[0025] 1) Take 10 grams of Ginkgo biloba powder that has been crushed and passed through an 80-mesh sieve, add 100 ml of choline eutectic solvent and 400 ml of water, and ultrasonically extract at 60°C for 40 minutes, power 10W / cm 2 , The frequency is 80kHz, and the extract is centrifuged at 4000rpm for 5 minutes to obtain the flavonoid extract;

[0026] 2) The flavonoids extract obtained in step 1) is filtered through a 0.45 μm filter membrane to remove impurities to obtain a filtered extract.

[0027] The obtained extract was tested by injecting 10 μL with a high performance liquid chromatograph, and the extraction amount of the target substance was calculated to be 280 mg / 10 g.

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Abstract

The invention discloses a method for extracting flavonoids from ginkgo biloba leaves with a deep eutectic solvent. The ginkgo biloba leaves are used as raw materials, the choline deep eutectic solvent is used as an extracting agent, water is used as a diluent for decreasing the system viscosity, the flavonoids are extracted under the ultrasonic intensification, the choline deep eutectic solvent is a room-temperature molten salt liquid of ethylene glycol-oxalic acid-choline chloride, and the molar ratio of ethylene glycol to oxalic acid to choline chloride in the room-temperature molten salt liquid is 1:1:1. The method has the advantages as follows: the extraction method has the characteristics that the extracting agent does not explode and volatilize, the loss is low, the energy consumption is low, potential risks are avoided, the operation is convenient, industrialization is easy, the efficiency is high and the like, and the method can be applied to efficient and green large-scale production of the flavonoids and realization of comprehensive utilization of ginkgo biloba leaf resources.

Description

Technical field [0001] The invention relates to biochemical engineering technology, in particular to a method for extracting flavonoids from ginkgo leaves by using a eutectic solvent. technical background [0002] Ginkgo biloba is the dry leaves of Ginkgo biloba (Ginkgo biloba L.), harvested in autumn when the leaves are still green. The function of ginkgo biloba is to promote blood circulation, remove blood stasis, dredge collaterals and relieve pain. Now it is mainly used clinically for cardiovascular diseases. The extraction of pharmaceutical ingredients from ginkgo biloba began in the 20th century. The product EGB761 developed by Schwabe Pharmaceuticals in Germany and its standard preparation method further promoted the research process of ginkgo biloba. A variety of chemical components have been isolated from Ginkgo biloba leaves, and flavonoids are relatively high content components in Ginkgo biloba leaves and are currently considered to be one of the effective components...

Claims

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Application Information

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IPC IPC(8): A61K36/16
Inventor 朱涛唐为扬
Owner TIANJIN UNIVERSITY OF TECHNOLOGY
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