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Preparation method of quinatic acid

A woody acid and drying technology, applied in the biological field, can solve the problems of low product purity, low extraction rate of plant active ingredients, unsuitable for industrial production, etc., and achieve high separation efficiency, good regeneration performance, and long service life.

Inactive Publication Date: 2015-09-16
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The product obtained by this type of method has low purity, and the extraction rate of plant active ingredients is not high, so it is not suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take Akebia, dry it at 60°C, crush it, and pass it through a 20-mesh sieve; put the above-mentioned coarse powder into a dilute acid solution with a pH value of 4 and a solid-to-liquid ratio of 1:0.5, and add 0.1% fiber by weight of the coarse powder to it. enzymatic hydrolysis for 1 h at a temperature of 45°C; put the obtained enzymatic hydrolysis mixture in a microwave extractor with a power of 1000W, add 70% ethanol with a solid-liquid ratio of 1:2, and heat at a temperature of 60 Extract under the condition of ℃ for 300s to obtain the crude extract of aketoic acid; the crude extract of amusic acid is first separated with a 0.4 micron ceramic membrane at a temperature of 20 ℃; then the obtained extract On the X-5 macroporous adsorption resin column, first elute with 2 times column volume of distilled water, then 2 times column volume, 40% ethanol, and finally 2 times column volume, 80% ethanol, Collect the ethanol eluate and filter it with a 0.1 micron organic microp...

Embodiment 2

[0020] Take Akebia, dry at 65°C, crush, and pass through a 60-mesh sieve; put the coarse powder into a dilute acid solution with a pH value of 4 and a solid-to-liquid ratio of 1:1, and add 0.2% pectin by weight of the coarse powder to it Enzyme, enzymolysis at 45°C for 1.5h; put the obtained enzymolysis mixture in a microwave extractor with a power of 2000W, add ethanol with a solid-liquid ratio of 1:4 and 75%, and heat it at a temperature of 80 Extract under the condition of ℃ for 600s to obtain the crude extract of aketoic acid; the crude extract of amusic acid is first separated with a 0.4 micron ceramic membrane under the condition of a temperature of 30 ℃; then the obtained extract On the LSA-21 macroporous adsorption resin column, first elute with 3 times column volume of distilled water, then 3 times column volume, 50% ethanol, and finally 4 times column volume, 85% ethanol, Collect the ethanol eluent and filter it with a 0.3 micron organic microporous membrane, and det...

Embodiment 3

[0022] Take Akebia, dry it at 70°C, crush it, and pass through a 80-mesh sieve; put the above-mentioned coarse powder into a dilute acid solution with a pH value of 5 and a solid-to-liquid ratio of 1:1, and add 0.3% of the weight of the coarse powder to it. Cellulase, enzymatic hydrolysis at 50°C for 2 hours; put the obtained enzymatic hydrolysis mixture in a microwave extractor with a power of 2000W, add ethanol with a solid-liquid ratio of 1:5, and 80% at a temperature of Extract at 90°C for 900s to obtain the crude extract of aketoic acid; firstly, use a 0.4 micron ceramic membrane to separate part of the impurities from the crude extract of amusic acid at a temperature of 40°C; then extract the obtained The WLD type macroporous adsorption resin column on the liquid was first eluted with distilled water of 3 times the column volume, then eluted with 4 times the column volume and 60% ethanol, and finally eluted with 5 times the column volume and 90% ethanol, and collected Th...

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PUM

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Abstract

The invention discloses a preparation method of quinatic acid, in particular to a method of extracting quinatic acid by a biological enzyme enzymolysis assisted microwave extraction technique. The specific process steps include: raw material treatment, enzymolysis, microwave extraction, purification, vacuum concentrating, and freeze drying, thus obtaining the quinatic acid. The method provided by the invention utilizes biological enzyme enzymolysis, can fully destroy the cell wall of the plant to make the effective ingredients fully flow out, also the added acid water enhances the biological enzyme activity and also makes the quinatic acid free, thus enhancing the quinatic acid yield. A ceramic membrane and macroporous adsorption resin column are employed to purify quinatic acid so as to well enhance the purity of the obtained product. The method provided by the invention has the advantages of simple process, mild reaction conditions, high selectivity, high purity quinatic acid and the like.

Description

technical field [0001] The invention discloses a preparation method of aketoic acid, which belongs to the field of biotechnology. Background technique [0002] Mutong acid is a triterpenoid component, molecular formula C 30 h 32 o 4 , whose structure is 3α,24-dihydroxy-30-noroleanane-12,20 (29 )-diene-28-acid, aketoic acid is the exclusive component of akebia, which has anti-inflammatory activity. Akebia is a plant of the genus Akebia, bitter in taste, slightly cold in nature, and has the effects of clearing heart-fire, diuresis, and stimulating the menstrual flow. Indications for restlessness and heat in the chest, sore throat, red urine, five stranguria, edema, body cramps, amenorrhea and less breasts. Modern pharmacological studies have shown that Akebia has diuretic, antibacterial and antitumor activities. [0003] Ceramic membranes, also known as inorganic ceramic membranes, are asymmetric membranes prepared from inorganic ceramic materials through special processe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00
CPCC07J63/008
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH
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