Method for enhancing collagen secretion and preventing cutaneous aging using chenopodium formosanum extract

A collagen production, collagen technology, applied in the direction of drug combination, skin care preparations, food preparation, etc., to achieve the effect of resisting UV light damage, preventing glycation, and promoting secretion rate

Active Publication Date: 2015-09-23
百岳特生物科技(上海)有限公司 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no product on the market that is safe, effective, and can simultaneously promote collagen production, prevent collagen saccharification, and resist UV light damage to achieve a full range of skin anti-aging protection effects, especially food products.

Method used

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  • Method for enhancing collagen secretion and preventing cutaneous aging using chenopodium formosanum extract
  • Method for enhancing collagen secretion and preventing cutaneous aging using chenopodium formosanum extract
  • Method for enhancing collagen secretion and preventing cutaneous aging using chenopodium formosanum extract

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0023] Preparation of red quinoa extract

[0024] The red quinoa (Chenopodium formosanum) used in the present invention is derived from Pingtung County, and the whole grain (unhulled) red quinoa is stored at 4° C. for later use. For the preparation of red quinoa extract, please refer to figure 1 . In order to achieve a better extraction effect, before carrying out the extraction step of the present invention, the red quinoa can be made into smaller particles as much as possible by physical methods such as mashing, grinding, chopping, etc., preferably ground way to facilitate the subsequent extraction.

[0025] Once the red quinoa is solvent extracted, the extract can be formulated for a wide variety of applications. However, in order to improve the purity of the active ingredient in the extract, various purification steps may be carried out after the extraction step of the present invention if necessary. The method for purifying the extract does not need to be specially ta...

Embodiment 1

[0032] Example 1: Analysis of the ability of red quinoa to promote collagen secretion rate

[0033] First, human skin fibroblasts CCD-966SK (Human skin fibroblast cells) were cultured in a 24-well plate in minimum essential medium (MEM) containing 10% fetal bovine serum (FBS), and the concentration of human skin fibroblasts was 4 ×10 4 cells / well. The medium was refreshed after 24 hours of incubation. The red quinoa extract was diluted to 0.2 mg / mL with phosphate buffer saline (PBS) and added to each well. Cells without red quinoa extract served as the control group. After the cells were co-cultured with the red quinoa extract for 48 hours at 37°C and 5% carbon dioxide, the supernatant was taken. use Collagen Assay Kit (Bicolor Life Science Assays, Northern Ireland, UK) was used to analyze the total content of water-soluble type I collagen. At room temperature, mix 100 μL of the supernatant obtained by the above method with 1 mL The dye was mixed for 30 minutes. This...

Embodiment 2

[0036] Example 2: Analysis of the ability of red quinoa extract to prevent collagen glycation

[0037] Regarding the analysis of the red quinoa extract of the present invention inhibiting collagen saccharification, at first porcine collagen and fructose were prepared into 60 mg / mL and 1.5 mg / mL respectively with 0.2M phosphate buffer solution (containing 0.06% sodium azide, pH 7.4). M's solution. In order to produce collagen glycosylation, 100 μL of porcine collagen solution, 100 μL of fructose solution, and 100 μL of deionized water were co-cultured in a 1.5 mL microcentrifuge tube, and stored frozen at -20 °C after co-cultivation. Glycation analysis of collagen and fructose was carried out with a glycation inhibitor: aminoguanidine (AG, purchased from Sigma). 100 μL of 3 mM aminoguanidine solution (prepared in deionized water) was added to the solution of the above saccharification reaction instead of deionized water. At the same time, different relative concentrations of ...

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Abstract

Provided is a method for enhancing collagen secretion and preventing cutaneous aging using Chenopodium formosanum extract. The Chenopodium formosanum extract includes active ingredients that are able to effectively enhance the ability of collagen secretion of dermal fibroblasts as well as prevent the glycation of collagen and resist the damage of ultraviolet light exposure. The preparation of the Chenopodium formosanum extract is environmental friendly and is able to promote the economic value of Chenopodium formosanum; thus, the present invention is suitable for applications in the development of food products, especially beverages, skin care and cosmetic products.

Description

technical field [0001] The present invention relates to the use of a red quinoa extract, in particular to the use of a red quinoa extract for preparing a composition for promoting collagen production and anti-aging skin, which can effectively promote the secretion rate of collagen and prevent collagen saccharification Function, and resistance to UV light damage. Background technique [0002] Red quinoa (Chenopodium formosanum), also known as Taiwan quinoa, red quinoa, purple quinoa, rainbow rice, belongs to a kind of annual or perennial herbaceous plant under Chenopodium genus Chenopodium formosanum, which is a unique plant in Taiwan. Its growth characteristics are drought-tolerant, High salinity is mainly distributed in the middle and low altitude mountainous areas of central and southern Taiwan. Red quinoa is edible, and its seedlings, tender stems and leaves and flower spikes can be added to cooking or cooking soup, and the seeds and shells are separated and ground into ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P17/18A23L1/29A23L2/38A23C9/152A23L1/06A61K8/97A61Q19/00A61Q19/10A61Q17/04A61Q1/02
CPCA23V2002/00A23L1/3002A61K8/97A61Q19/08A61K2800/522A61K2800/92A61K8/9789A61P17/16A61P17/18
Inventor 苏香绫余锦秀陈盈如
Owner 百岳特生物科技(上海)有限公司
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