Microtubule biochip based on photonic crystal microspheres

Abstract

The invention discloses a microtubule biochip based on photonic crystal microspheres. The microtubule biochip is composed of a top cover, an eyelet-carrying panel, a flat-ended test tube and an independent soft rubber cover. Microspheres used for detecting a carrier are loaded in the flat-ended test tube. The microspheres have photonic crystal codes, which can be decoded in the way of measuring reflective peak position by a fiber optic spectrometer or distinguishing colors by the naked eye. A probe, which could be nucleic acid or protein, is fixed to the surface of the microspheres. The pretreated microspheres with the different codes can move freely at the bottom of the test tube. According to the invention, a photonic crystal coding liquid chip technology is taken as a basis and integrated in the microtubule chip device, thus avoiding breakdown and loss of the photonic crystal microspheres during experiment operations and raising repeatability and accuracy of experimental data.

Description

technical field [0001] The invention relates to a microtube biochip based on photonic crystal microspheres. It can be used to detect biological molecules such as proteins and nucleic acids, and can be widely used in clinical testing, environmental monitoring, inspection and quarantine, microbial identification, drug screening, and functional analysis of nucleic acids and proteins. Background technique [0002] At present, enzyme immunoassay has become the most widely used immunoassay technique in clinical testing due to its advantages of simple operation, no need for expensive equipment, no pollution to the environment, easy preparation of enzyme-labeled reagents, stable properties, and long validity period. However, as the main carrier of enzyme immunoassay, the 96-microwell plate is coated with only one antigen or antibody per well, resulting in a kit that can only detect one antibody or antigen. If different antibodies or antigens are detected, different kits are needed,...

AI Technical Summary

Problems solved by technology

In the multiple detection repeated experiments based on photonic crystal encoded microspheres, it is difficult to distinguish the microspheres by naked eyes, so in the non-chip detection mode, the microspheres are often artificially damaged or lost.

The experimental data is the average value of the data measured by multiple microspheres, so the loss of microspheres will largely affect the accuracy and repeatability of the experimental data

At the same time, the random experimental error between different operators brought about by the non-chip detection mode is uncontrollable, which further affects the accuracy and repeatability of the method

Images