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Method for fermenting dendrobium officinale employing lactic acid bacteria, as well as fermented product and application of dendrobium officinale

A technology of lactic acid bacteria fermentation and Dendrobium candidum, which is applied in the field of microbial fermentation, can solve the problems of single efficacy and few varieties of Dendrobium candidum products, and achieve the effects of improving taste, enriching product categories, increasing nutritional value and health care efficacy

Inactive Publication Date: 2015-09-30
广东罗特制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a lactic acid bacteria fermentation method of Dendrobium candidum and its fermentation products and applications, aiming to solve the problems of the existing Dendrobium candidum products with few varieties and single efficacy.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1. Fresh strips of Dendrobium officinale use a clean toothbrush to remove the cuticle of the epidermis, wash and dry; cut it into 0.5-2cm segments with scissors, weigh them, and prepare purified water at a weight ratio of 1:8; Put the stem section into a juicer, add an equal amount of purified water to extract the juice; use 100-mesh nylon gauze as a medium for vacuum filtration, wash the filter residue twice with the remaining purified water, and collect the filtrate for later use.

[0051] 2. According to the proportion of 5% inoculum, the bacterial strain was inserted into the fresh juice of Dendrobium candidum, and sealed and fermented for 20 hours at 32° C. and 100 r / min.

[0052]3. After the fermentation is over, add 8% trehalose to the fermentation product as a protective agent, mix and dissolve; freeze-dry at a vacuum degree of 12Pa and a temperature of ~63°C to reduce the moisture content to below 6%, that is, rich in active probiotics Dendrobium candidum ferme...

Embodiment 2

[0054] 1. Fresh strips of Dendrobium officinale use a clean toothbrush to remove the cuticle of the epidermis, wash and dry them; cut them into 0.5-2cm sections with scissors, weigh them, and prepare purified water at a weight ratio of 1:5; Put the stem section into a juicer, add an equal amount of purified water to extract the juice; use 100-mesh nylon gauze as a medium for vacuum filtration, wash the filter residue twice with the remaining purified water, and collect the filtrate for later use.

[0055] 2. According to the proportion of 8% inoculum, the bacterial strain was inserted into the fresh juice of Dendrobium officinale, and sealed and fermented for 16 hours under the conditions of 35° C. and 150 r / min.

[0056] 3. After the fermentation is over, add 10% trehalose to the fermentation product as a protective agent, mix and dissolve; freeze-dry at a vacuum degree of 15Pa and a temperature of -60°C, so that the moisture content is reduced to below 6%, that is, rich in ac...

Embodiment 3

[0058] 1. Fresh strips of Dendrobium candidum, remove the cuticle of the epidermis with a clean toothbrush, wash and dry, and cut it into 2-5cm sections with scissors; freeze-dry under the condition of vacuum degree of 8Pa and temperature of -63℃ to reduce the water content to less than 5%; pulverize to 200 mesh with an ultrafine pulverizer.

[0059] 2. Use sterile water to adjust the water content of Dendrobium officinale superfine powder to 70%, and the pH is natural; according to the proportion of inoculum 6%, insert probiotic strains into the suspension of Dendrobium officinale superfine powder, and ferment for 20 hours at a temperature of 32°C .

[0060] 3. After the fermentation is over, add 5% trehalose to the fermentation product as a protective agent, mix and dissolve; freeze-dry at a vacuum degree of 8Pa and a temperature of -63°C, so that the moisture content is reduced to below 6%, that is, rich in active probiotics Dendrobium candidum fermentation product.

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Abstract

The invention discloses a method for fermenting dendrobium officinale employing lactic acid bacteria, as well as a fermented product and application of the dendrobium officinale. The method comprises the following steps: squeezing fresh dendrobium officinale strips into juice, or carrying out freeze drying and superfine grinding; fermenting by employing lactic acid bacteria as a dendrobium officinale fermenting strain; and finishing fermentation, adding mycose to a fermented product as a protective agent, and carrying out freeze drying, so as to obtain the fermented product with activated probiotics. The dendrobium officinale is fermented with the lactic acid bacteria; the activated probiotics are introduced on the basis of the original health efficacy of the dendrobium officinale; the mouthfeel and flavor of the dendrobium officinale are improved; the nutrient value and health efficacy of the dendrobium officinale are improved; the variety of dendrobium officinale series products is enriched; the content of the probiotics of the fermented dendrobium officinale is not less than 109.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a lactic acid bacteria fermentation method of dendrobium officinale, a fermentation product and application thereof. Background technique [0002] Dendrobii candidum (Dendrobii candidum) is a perennial epiphytic herb of Orchidaceae. It is listed as "the first of the nine immortal grasses in China". "Chinese Pharmacopoeia" records the specific effects of benefiting the stomach and promoting body fluid, nourishing yin and clearing away heat. In addition, it also has unique effects in enhancing the body's immunity, anti-fatigue, promoting the secretion of gastric juice to aid digestion, protecting the liver and gallbladder, enhancing hypoglycemia, and anti-tumor. [0003] Probiotics have the functions of anti-mutagenesis, anti-cancer, improving lactose metabolic rate, lowering serum cholesterol and stimulating the immune system in the human intestinal tract. In addi...

Claims

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Application Information

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IPC IPC(8): A61K36/8984A61K35/744A23L1/29
Inventor 罗楚波杨善岩
Owner 广东罗特制药有限公司
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