Microfluidic chip for pcr and virus rapid detection device for real-time pcr
A microfluidic chip and detection device technology, which is applied in enzymology/microbiology devices, biochemical cleaning devices, microbial measurement/inspection, etc., can solve the problems of unsuitability, slow temperature rise and fall in the reaction zone, and unfavorable problems. Achieve the effect of cost saving, small sample dose, and portability
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Embodiment 1
[0031] Referring to FIG. 1 , the microfluidic chip for implementing PCR provided in this embodiment includes a reaction layer and a pneumatic control layer 20 disposed above the reaction layer. Among them, see Figure 1a and Figure 1b , the reaction layer includes a substrate 110 and a film layer 120 covering the substrate 110 . Wherein, the substrate 110 is made of hard transparent material such as glass, and the film layer 120 is made of elastic material. In a preferred embodiment, the film layer 120 is made of polydimethylsiloxane (polydimethylsiloxane, PDMS) material, which has the advantages of good optical transparency, good adhesion, and chemical inertness. The side of the film layer 120 facing the substrate 110 is provided with a sample tank 123 for containing samples and a reaction zone 121 arranged around the sample tank 123, wherein the sample enters the sample tank through the sample injection hole 124 arranged in the sample tank 123 Within 123. In this embodim...
Embodiment 2
[0040] Referring to FIG. 3 , the microfluidic chip for implementing PCR provided in this embodiment includes a reaction layer and a pneumatic control layer 20 disposed above the reaction layer, wherein the reaction layer and the pneumatic control layer 20 have the same structure as in Embodiment 1. The difference from Example 1 is that the film layer 120 in the reaction layer provided in this example is made of PMDS material, which is coated with air-tight material such as parylene in the reaction tank 1211 and flow channel 122. Formed isolation layer, the isolation layer is used to prevent the evaporation of water in the reaction zone 121.
[0041] In the microfluidic chip provided in this embodiment, the water injection layer is replaced by the isolation layer arranged in the reaction tank 1211 and the flow channel 122, thereby suppressing the evaporation of water in the reaction zone 121, which is not only simple in structure and easy to use, but also reduces the cost.
Embodiment 3
[0043] Referring to Fig. 4, the microfluidic chip for implementing PCR provided in this embodiment includes a reaction layer, wherein the reaction layer includes a bottom plate 130 and a cover plate 140 covered on the bottom plate 130, and the bottom plate 130 and the cover plate 140 are all made of plastic. material. The side of the bottom plate 130 facing the cover plate 140 is provided with a sample tank 123 for containing samples and a reaction zone 121 arranged around the sample tank 123, wherein the sample passes through the position of the cover plate 140 relative to the sample tank 123. The sample hole 124 enters into the sample tank 123 . In this embodiment, the reaction layer includes 12 groups of reaction zones 121 , wherein each group of reaction zones 121 includes three reaction tanks 1211 , and each reaction tank 1211 is connected to the sample tank 123 through a flow channel 122 . Preferably, the position of the reaction zone 121 is higher than the sample tank ...
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Abstract
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