Double-liquid-phase fermentation method of monascus yellow pigment by coupled in-situ fermentation-extraction

A technology of monascus yellow pigment and two-liquid phase, which is applied in the field of fermentation engineering, can solve the problems of unsuitable industrial production, low concentration of fermentation pigment, and long fermentation time, so as to improve permeability, shorten fermentation time, and reduce negative feedback The effect of inhibition

Active Publication Date: 2015-09-30
JIANGNAN UNIV
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Problems solved by technology

The above cases do not meet the requirements of industrial production due to the low concentration of fermentation pigment and long fermentation time.

Method used

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  • Double-liquid-phase fermentation method of monascus yellow pigment by coupled in-situ fermentation-extraction

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Experimental program
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Effect test

Embodiment 1

[0030] Monascus JJLY-4A was selected as the shake flask strain. The fermentation medium consists of a minimal medium and an extract phase. Basic medium (g / L): corn starch 60, soybean powder 2, ammonium sulfate 8, NaNO36, yeast extract 0.2, corn steep liquor powder 0.2, zinc sulfate 0.2, magnesium sulfate 0.8, potassium dihydrogen phosphate 1.2; extract phase (with Volume ratio of medium): 10% soybean oil, 8% corn oil, 1% tricaprylic glyceride, 0.3% dicaprylic glyceride, and 0.2% caprylic glyceride. Except for soybean oil and corn oil in the extraction phase, the rest of the glycerides were added to the medium 48 hours after the fermentation started.

[0031] The liquid seeds of Monascus in the logarithmic growth phase were placed in a 500mL Erlenmeyer flask containing 100mL of fermentation medium at an inoculum volume of 10% by volume, and the shake flask was fermented at a speed of 180r / min and a temperature of 30°C. 5d. After the end, it is measured that the color value o...

Embodiment 2

[0033] The experimental procedure and fermentation medium are the same as those of the above-mentioned Example 1, except that the experimental strain is Monascus ZH6. Put the liquid seeds of Monascus in the logarithmic growth phase into a 500mL Erlenmeyer flask containing 100mL of fermentation medium at an inoculation volume of 10% by volume, and ferment in a shaker flask at a speed of 180r / min and a temperature of 30°C Culture 5d. After the end, it is measured that the color value of the monascus yellow pigment converted to the volume of the basic medium is between 160-170U / mL.

Embodiment 3

[0035] Monascus JJLY-4A was used as shake flask fermentation strain. It is basically the same as above-mentioned embodiment 1 step and culture medium formula, difference is: the cornstarch in the fermentation medium is 40g / L. Under the conditions of rotation speed of 180r / min and temperature of 30°C, shake flask fermentation culture was carried out for 5 days. After the end, it is measured that the color value of the monascus yellow pigment converted to the volume of the basic medium is between 150-160U / mL.

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Abstract

The invention discloses a double-liquid-phase fermentation method of monascus yellow pigment by coupled in-situ fermentation-extraction, belonging to the field of fermentation engineering. The varieties and contents of carbon and nitrogen sources in the fermentation culture medium are controlled to effectively control the monascus pigment biosynthesis pathway, so that citraurin generated by the monascus is no longer converted into the monascus red pigment; and the double-liquid-phase extraction-fermentation coupled system can ensure the normal growth and fermentation of the monascus, is beneficial to enhancing the dissolved oxygen level, and is capable of promoting the secretion of the monascus pigment to the outside of cells and lowering the negative feedback inhibiting action of the monascus fermentation product, thereby greatly enhancing the fermentation level of the monascus yellow pigment and shortening the fermentation time. After the fermentation finishes, the product can be easily separated. The method can greatly enhance the yield and production efficiency of the monascus yellow pigment and lower the production cost.

Description

technical field [0001] The invention relates to a two-liquid-phase fermentation method for coupling in-situ extraction and fermentation of monascus yellow pigment, belonging to the field of fermentation engineering. Background technique [0002] Natural yellow pigments are divided into plant extracts and microbial fermentation products. At present, the natural yellow pigments that can be produced by large-scale microbial fermentation are mainly carotenoids and monascus yellow pigments. Monascine is divided into two types, one is monascine and the other is ankaflavine. The wavelength of the maximum absorption peak of monascus yellow is generally 400-440nm. In addition, there is also a yellow pigment on the market that is also derived from the monascus red pigment but has been chemically modified, that is, the natural monascus red pigment is used as a raw material, and it is reacted with sodium dithionite in an alkaline solution to make the monascus red pigment lactone Hydr...

Claims

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Application Information

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IPC IPC(8): C12P17/18C09B61/00C12R1/66
Inventor 许赣荣毛鹏张薄博黄艳贾虎斌
Owner JIANGNAN UNIV
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