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Rice leaf roller acquisition and biological assay method

A rice leaf roller and bioassay technology, applied in the field of agricultural science research, can solve the problems of large number of test insects, difficult breeding, high technical requirements, etc., to reduce collection costs, overcome physiological differences, and reproduce test results good sex effect

Inactive Publication Date: 2015-10-07
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the difficulty of indoor breeding of the rice leaf roller, the high technical requirements for the standard test insects and the large number of test insects in the bioassay of insecticides, and the development of control agents for the simple and easy-to-use rice leaf roller In the urgent need of bioassay technology, the present invention provides a method for easily collecting adults of the rice leaf roller from the field, and quickly delivering the adults collected in the field to a bioassay laboratory, and using newly hatched larvae for insecticide screening biometric technology

Method used

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  • Rice leaf roller acquisition and biological assay method
  • Rice leaf roller acquisition and biological assay method

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Example 1: Method for collecting and supplementing nutrient supply of rice leaf roller adults

[0022] During the occurrence period of rice leaf roller, use insect nets to catch rice leaf roller adults from paddy field nets at 6-8 o'clock in the morning and 5-7 o'clock in the afternoon every day. 20 cm, one end is sealed with a nylon net) into a transparent plastic cup (350-500 ml), 2 to 4 pairs of male and female adults per cup, the plastic cup is sealed with a plastic wrap, and some small air holes are inserted on the plastic wrap with a needle. Paste a piece of double-sided tape of 1 square centimeter on the bottom of the plastic cup before collecting adults, fix a small ball of cotton balls on the bottom of the plastic cup with double-sided tape, and add 10% sugar water or honey water prepared in advance to the cup The cotton balls are used for the rice leaf roller adults to feed, and the adults directly lay eggs in the plastic cup after feeding. The plastic cups c...

Embodiment 2

[0023] Embodiment 2: The delivery method of rice leaf roller adults and eggs

[0024] The rice leaf roller adults collected by the method described in Example 1 can be sent by express delivery, and the adults to be sent should use transparent plastic cups with higher strength when collecting, and use foam or foam between the plastic cups in the box. Filled with paper scraps to prevent extrusion and impact during delivery. The packaging box should have a certain anti-extrusion and anti-drop strength, and a small number of ventilation holes are opened around the box. The adults collected the night before and the morning of the day can be sent together. After the laboratory receives the adults and eggs, if the eggs have not yet hatched, they can be incubated in the laboratory until they can be directly measured after hatching.

Embodiment 3

[0025] Example 3: Toxicity of different insecticides to rice leaf roller. The toxicity of 10 insecticides to rice leaf roller populations in Nanning, Guangxi, Changsha, Hunan, and Nanjing, Jiangsu was measured by using the rice seedling dipping method introduced in the present invention. The results are shown in Table 1. Among the 10 insecticides tested, abamectin, emamectin benzoate and spinosad have the highest toxicity to rice leaf roller. 50 The values ​​are all below 0.02 mg active ingredient / liter. The next most toxic insecticides are chlorantraniliprole, chrysantraniliprole, chlorpyrifos and quinathion, and their LC 50 The value is between 0.3 and 2.0. Monosultap and Bt had the lowest toxicity to the larvae of the rice leaf roller.

[0026]

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Abstract

The invention discloses an assay method for field imago acquisition and ovum collection of rice leaf rollers and the virulence of pesticides to rice leaf rollers. During the imago occurrence period of the rice leaf rollers, rice filed imagoes are netted into a plastic cup which is sealed by a preservative film, cotton balls are fixed at the bottom in the cup through double faced adhesive tapes, sugar water or hydromel supplements nutrition to the imagoes, the imagoes spawn on the inner wall of the plastic cup, the ovum hatched larvae are directly subjected to bioassay, the larvae don't have to be bred indoors, and the rice seedling impregnation method for rice seedlings cultivated for 2-4 weeks (the height of 20-25cm) is adopted for determination. The method prevents larva breeding and overcomes the problem that the rice leaf rollers cannot be bred for large scale and medicament screening cannot be carried out; and moreover, the invention provides a simple and easy rice leaf roller virulence determination method for pesticide research and development organizations, so that the biological assay of the rice leaf rollers, the mixed formulation screening, and resistance monitoring are possible.

Description

technical field [0001] The invention belongs to the field of agricultural science research, and in particular relates to a method for collecting rice leaf rollers from fields and measuring the toxicity of insecticides to rice leaf rollers. Background technique [0002] The rice leaf roller is an important migratory pest in rice growing areas in Asia, causing serious economic damage and loss to rice production all year round. The damage of rice leaf roller to rice is by rolling the leaves on the leaves and feeding on the leaf epidermis, which affects photosynthesis and reduces rice yield. In production, the control of rice leaf rollers is mainly based on the use of chemical insecticides, which requires scientific research institutions and pesticide companies to develop insecticide varieties and mixtures that are highly effective against rice leaf rollers. [0003] Because the large-scale indoor breeding of rice leaf roller is still difficult at present, and the existing bree...

Claims

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Application Information

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IPC IPC(8): A01M1/10A01K67/033A01M1/20A01G7/06
Inventor 苏建亚郑雪松任秀贝张树坤王业成
Owner NANJING AGRICULTURAL UNIVERSITY
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