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Preparation method of quantum dot/enzyme composite carbon paste electrode for detection of SAM

A technology of quantum dots and composite carbon, which is applied in the field of preparation of quantum dots/enzyme composite carbon paste electrodes, to achieve the effects of wide electrochemical window, surface renewal, and small residual current

Inactive Publication Date: 2017-06-23
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The bio-enzyme electrode sensor is currently developed with the characteristics of specificity, stability, fast detection speed, good selectivity, and high sensitivity. It is widely used in the fields of clinical medicine, food, environment, and biological sample detection. The detection of SAM on carbon paste electrode has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Preparation of semiconductor precursor solution: In the reactor, add 1.0g of tellurium powder, 0.9g of sodium borohydride, 50mL of deionized water, under the protection of argon, ultrasonically disperse for 10 min, and hold at 60℃. The reaction was refluxed for 5 hours, cooled to room temperature, and placed in an ice water bath to obtain a semiconductor precursor solution;

[0030] (2) Preparation of zinc-cadmium precursor solution: In the reactor, add cadmium chloride: 0.6g, zinc chloride: 0.5g, cysteine: 0.9g, water: 98mL, and adjust the pH of the solution with sodium hydroxide. It is 10, argon gas is introduced, the reaction is refluxed at a constant temperature of 85°C for 4 hours, and then cooled to room temperature to prepare a zinc-cadmium precursor liquid;

[0031] (3) Preparation of water-soluble CdZnTe quantum dots: Add the semiconductor precursor solution prepared in step (1) to the zinc-cadmium precursor solution prepared in step (2) under the protection of ...

Embodiment 2

[0035] (1) Preparation of semiconductor precursor solution: In the reactor, add 0.5g of tellurium powder, 0.6g of sodium borohydride, 30mL of deionized water, under the protection of argon, ultrasonically disperse for 10 min, and hold at 60℃. The reaction was refluxed for 4 hours, cooled to room temperature, and placed in an ice-water bath to obtain a semiconductor precursor solution;

[0036] (2) Preparation of zinc-cadmium precursor solution: In the reactor, add cadmium chloride: 1.0g, zinc chloride: 0.8g, cysteine: 1.2g, water: 97mL, and adjust the pH of the solution with sodium hydroxide. It is 10, argon gas is introduced, and the reaction is refluxed at 80°C for 5 hours, and then cooled to room temperature to prepare zinc-cadmium precursor liquid;

[0037] (3) Preparation of water-soluble CdZnTe quantum dots: Add the semiconductor precursor solution prepared in step (1) to the zinc-cadmium precursor solution prepared in step (2) under the protection of argon, and react at a co...

Embodiment 3

[0041] (1) Preparation of semiconductor precursor solution: In the reactor, respectively add 0.8g tellurium powder, 0.5g sodium borohydride, 40mL deionized water, under the protection of argon, ultrasonically disperse for 10 min, and hold at 60℃. The reaction was refluxed for 6 hours, cooled to room temperature, and placed in an ice-water bath to obtain a semiconductor precursor solution;

[0042] (2) Preparation of zinc-cadmium precursor solution: In the reactor, add cadmium chloride: 0.2g, zinc chloride: 0.2g, cysteine: 0.6g, water: 99mL, and adjust the pH of the solution with sodium hydroxide. It is 10, argon gas is introduced, the reaction is refluxed at 85°C for 4 hours, and cooled to room temperature to prepare zinc-cadmium precursor liquid;

[0043] (3) Preparation of water-soluble CdZnTe quantum dots: The semiconductor precursor solution prepared in step (1) is added to the zinc-cadmium precursor solution prepared in step (2) under the protection of argon, and the solution ...

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Abstract

The invention discloses a method for preparing a quantum dot / enzyme composite carbon paste electrode for detecting SAM, which is characterized in that: carbon paste electrodes are doped with water-soluble CdZnTe quantum dots and methyltransferase, and 1-ethyl ‑3‑Methylimidazole hexafluorophosphate is used as an adhesive to prepare a carbon paste electrode by mixing carbon nanotubes and graphite powder, which is a quantum dot / enzyme composite carbon paste electrode. Compared with ordinary carbon paste electrodes, the conductivity is improved by 1~2 times, and the electrochemical window is wide, the preparation method is simple, the cost is low, the surface is easy to renew, and the residual current is small; the electrode is used to quickly detect SAM in the sample, and the method has high sensitivity. Good selectivity, short response time, less interference, superior to other detection methods, is a simple, fast, convenient and easy SAM determination method, the fixed methyltransferase electrode sensor prepared by the application has low cost, simple preparation process, specific It has good performance and has the possibility of realizing automatic on-site determination.

Description

Technical field [0001] The invention relates to a preparation method of an electrochemical sensor, in particular to a preparation method and application of a quantum dot / enzyme composite carbon paste electrode for detecting S-adenosylmethionine (SAM). Background technique [0002] S-adenosylmethionine (SAM), SAM contains active methyl groups, and almost all methyl groups used for methylation modification in cells are derived from SAM methylsulfide high-energy bonds. Due to the wide range of methylation reactions, it can be said that SAM is a coenzyme second only to ATP in the importance of participating in the reaction in the cell. A small change in the concentration of SAM in the cell will have a significant impact on the growth, differentiation and function of the cell influences. SAM in bacteria is mainly synthesized by SAM synthetase (MetK) through methionine (Met) and ATP. when E.coli When the level of SAM synthetase decreases, the cell will not divide normally when the me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/26
Inventor 李慧芝卢燕赵淑英
Owner UNIV OF JINAN
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