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Detection method for various kinds of trace glucocorticoid and derivate thereof in surface water body

A technology of glucocorticoid and detection method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of difficult detection, complex matrix, low content of glucocorticoid and its derivatives, etc., and achieve good and fast recovery effect Analysis, effect of low detection limit

Inactive Publication Date: 2015-10-07
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the difficulties existing in the prior art, the present invention provides an efficient, simple and economical analysis method for the complex matrix in the water environment, the extremely low content of glucocorticoids and their derivatives, and the difficulty in detection. Determination of common glucocorticoids and their derivatives in surface water samples

Method used

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  • Detection method for various kinds of trace glucocorticoid and derivate thereof in surface water body
  • Detection method for various kinds of trace glucocorticoid and derivate thereof in surface water body
  • Detection method for various kinds of trace glucocorticoid and derivate thereof in surface water body

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Embodiment 1

[0044] A detection method for the content of trace glucocorticoids and derivatives thereof in surface water, the method comprising the steps of:

[0045] (1) Pretreatment of surface water samples

[0046] Collect 1.5 L of surface water samples and filter them through Whatman GF / C glass fiber filter paper with a pore size of 1.2 μm, and then pass them through a hydrophile activated with 6 mL of ethyl acetate, 6 mL of acetonitrile and 6 mL of ultrapure water at a flow rate of 3–5 mL / min. Lipophilic balance (HLB) solid phase extraction column, after all the water samples pass through the solid phase extraction (SPE) column, rinse the SPE column with 12mL ultrapure water, and blow dry with weak nitrogen to remove the water in the extraction column, and finally use 6mL Elute the HLB solid-phase extraction column with ethyl acetate / acetonitrile (1:1, V / V), collect the eluate and dry it with weak nitrogen gas, redissolve it with 100 μL methanol and wait for the test. All extraction a...

Embodiment 2

[0071] A detection method for the content of trace glucocorticoids and derivatives thereof in surface water, the method comprising the steps of:

[0072] (1) Pretreatment of surface water samples

[0073] Collect 1.5L each of 5 water samples from some urban rivers in Beijing and place them in brown glass bottles. The water samples are first filtered through GF / C glass fiber filter paper with a pore size of 1.2 μm to remove impurities. Ethyl ester, 6mL acetonitrile and 6mL ultrapure water were activated, and then the filtered water sample passed through the solid phase extraction column at a flow rate of 3-5mL / min. After all the water samples passed through the HLB column, rinse the solid phase extraction column with 12mL ultrapure water column, and blow dry with nitrogen to remove the water in the extraction column, and finally use 6mL ethyl acetate / acetonitrile (1:1, V / V) to elute the HLB solid phase extraction column, collect the eluate and dry it with weak nitrogen, and use...

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Abstract

The invention discloses a detection method for various kinds of trace glucocorticoid and a derivate thereof in a surface water body. The detection method comprises the steps that 1, a water body sample is preprocessed, wherein solid phase extraction is carried out on the water body sample after the water body sample is filtered; 2, an ultra-high performance liquid chromatography-mass spectrometry series method is adopted for analyzing the content of trace glucocorticoid and the derivate thereof in the water body sample; 3, a standard curve is drawn, an external standard method is used for quantitative determination, and the concentration of the trace glucocorticoid and the derivate thereof in the water body sample through conversion. The detection method has the advantages of being easy and convenient to operate, environmentally friendly and low in detection limit.

Description

technical field [0001] The invention relates to a detection method of chemical substances, in particular to a method for detecting glucocorticoids and their derivatives (including natural glucocorticoids, esterified glucocorticoids and halogenated glucocorticoids) detection method. Background technique [0002] One type of endocrine disrupting substances has been the focus of attention in the field of environmental science in recent decades. It can interfere with the endocrine system of the body and affect the endocrine disrupting system of humans and animals. In recent decades, the incidence of female uterine cancer has increased, and children's sexual maturity has increased. Premature and neonatal immunity decline have been shown to be related to endocrine disrupting substances. As a class of endocrine disrupting substances, glucocorticoids and their derivatives are new types of endocrine disrupting substances that are widely concerned at present. Studies have found that ...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 郭文景常红吴丰昌
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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