Bacillus coagulans and high-density fermentation method thereof, and dry bacterium powder preparation method

A technology of Bacillus coagulans and Bacillus coagulans, which is applied in the field of microorganisms, can solve the problems affecting the popularization and application of Bacillus coagulans and high product prices, and achieve the effects of being conducive to product promotion, high viable bacteria count, and simple and stable production process.

Active Publication Date: 2015-10-14
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These unfavorable factors directly cause high product prices, whi

Method used

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  • Bacillus coagulans and high-density fermentation method thereof, and dry bacterium powder preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Screening of Bacillus coagulans

[0043] Weigh 2g of a soil sample near a dairy farm in Mashan, Wuxi, Jiangsu, add 98ml of sterile saline, heat-treat at 80°C for 10min, shake on a shaker at 37°C at 120rpm for 20min, absorb 1ml of the supernatant and add it to LB medium Strains were isolated by medium dilution coating. Through morphological observation, physiological and biochemical experiments, and 16S rDNA analysis of the strains, the genus and species of the strains can be effectively and accurately determined.

[0044]Finally, the strain numbered ZS-007 was obtained, which was identified as Bacillus coagulans.

[0045] ZS-007 was deposited in the China Center for Type Culture Collection on May 3, 2015, with the preservation number CCTCC M 2015273.

Embodiment 2

[0046] Example 2: 1m 3 Fermenter preparation

[0047] Strain activation: the strain obtained in Example 1 was inoculated on the slant of nutrient agar, cultured at 30° C. for 24 hours, then transferred to the slant of nutrient agar eggplant bottle by streaking, and cultured at 30° C. for 24 hours. The composition of nutrient agar slant medium and nutrient agar eggplant bottle are: peptone 10g / L, beef extract 3g / L, sodium chloride 5g / L, agar 15-20g / L, pH7.2-7.4.

[0048] Shake flask culture: inoculate the activated bacteria into the shake flask, shake and cultivate for 20 hours. Shake flask medium formula: peptone 10g / L, beef extract 3g / L, sodium chloride 5g / L, pH7.2-7.4, shake flask liquid volume 50mL / 500mL Erlenmeyer flask. Culture conditions: the rotation speed of the shake flask is 200r / min, and the temperature is 30°C.

[0049] Fermentation tank fermentation: collect 20 bottles of triangular flask liquid as seeds and insert 1m 3 Fermenter. 1m 3 The liquid volume of t...

Embodiment 3

[0051] Example 3: 3m 3 Fermenter preparation

[0052] The strain activation is the same as in Example 2.

[0053] Shake flask culture: inoculate the activated bacteria into the shake flask, shake and cultivate for 20 hours. Shake flask medium formula: peptone 10g / L, beef extract 3g / L, sodium chloride 5g / L, pH7.2-7.4, and the liquid volume of the shake flask is 20mL / 250mL Erlenmeyer flask. Culture conditions: the rotation speed of the shake flask is 200r / min, and the temperature is 30°C.

[0054] Seed tank expansion: collect 10 bottles of 250mL shake flask seed liquid for seed tank inoculation. Seed tank medium composition: peptone 10g / L, beef extract 3g / L, sodium chloride 5g / L, pH7.2-7.4. The volume of the seed tank is 30L, and the filling volume is 80%. Culture conditions: stirring speed 200r / min, ventilation rate 0.8vvm, temperature 30°C, culture time 20hr.

[0055] Fermentation tank fermentation: 3m 3 The liquid volume of the fermentation tank is 70%. Medium composi...

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Abstract

The invention relates to a Bacillus coagulans strain which is collected by China Center for Type Culture Collection, and the collection number is CCTCC M 2015273. By adopting the liquid fermentation technique, the Bacillus coagulans viable count in the fermentation liquid is 1.52*10<10>cfu/mL or above, the spore number is 1.38*10<10>cfu/mL or above, and the spore rate is 90.8% or above. In the dry bacterium powder obtained by spray drying, the viable count is 8.0*10<11>cfu/g or above. The dry bacterium powder can be mixed with starch, glucose or any other filler to obtain a Bacillus coagulans microecological preparation. The Bacillus coagulans has the characteristics of high fermentation density and high bacterium content, is beneficial to lowering the product cost, is convenient for popularization in the produce market, and has active meanings for promoting healthy livestock/poultry cultivation.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a bacillus coagulans, a high-density fermentation method thereof and the prepared dried bacteria powder. Background technique [0002] Probiotics are microbial agents made through a special process, containing microorganisms that are beneficial to the host. Microecological preparations are natural, non-toxic, and can promote the health of the host, and are increasingly widely used in the fields of human health care and animal health. [0003] Lactic acid bacteria and bacillus-based probiotics are the two most widely used species in the feed industry. Lactic acid bacteria mainly include lactic acid bacteria, bifidobacteria, etc., and bacillus mainly include Bacillus subtilis, Bacillus licheniformis, etc. [0004] Lactic acid bacteria microecological preparations play a role by producing lactic acid, changing intestinal pH, and regulating intestinal microenvironment. Such...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/07
CPCC12N1/20C12N1/205C12R2001/07
Inventor 张梁石孔泉辛瑜
Owner JIANGNAN UNIV
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