Apparatus and method for rapidly measuring sulfur dioxide residue level in Chinese herb

A technology for quantitative determination of sulfur dioxide, which is applied in the preparation of test samples, material excitation analysis, color/spectral property measurement, etc.

Active Publication Date: 2015-10-14
HEBEI UNIV OF CHINESE MEDICINE
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Problems solved by technology

[0004] The main purpose of the present invention is to overcome above-mentioned deficiencies in the prior art, establish a kind of highly specific, high-sensitivity alkali titration-fluores

Method used

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  • Apparatus and method for rapidly measuring sulfur dioxide residue level in Chinese herb
  • Apparatus and method for rapidly measuring sulfur dioxide residue level in Chinese herb
  • Apparatus and method for rapidly measuring sulfur dioxide residue level in Chinese herb

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Experimental program
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Example Embodiment

[0036] Example 1

[0037] To determine the content of sulfur dioxide in Asparagus, the steps are:

[0038] a. Standard curve: In the reaction cell, add 3mL of sodium hydroxide solution with a concentration of 5g / L, and then accurately inject and draw 0.0, 0.1, 0.3, 0.5, 0.7, 0.9, 1.1, 1.3, 1.5mL of 130mg· L -1 Sodium sulfite solution, at the same time, 380mg·L was injected precisely -1 Ammonium acetate solution 2mL, and diluted with purified water to 10mL, slowly add 134mg·L dropwise slowly while stirring -1 3mL of o-phthalaldehyde solution, ultrasonic stirring at 70℃ for 20min, cooling to room temperature, adding purified water to make the volume 25mL; after passing the membrane, pour into 1cm cuvette, measure absorbance A at wavelength 354nm, plot concentration and absorbance A The standard curve is as attached figure 1 ), get the regression equation: A=87.03+517.32X, R 2 = 0.998.

[0039] b. Sample determination: accurately weigh 100mg of Chinese herbal medicine Asparagus into a ...

Example Embodiment

[0047] Example 2

[0048] To determine the content of sulfur dioxide in Fritillaria, the steps are:

[0049] Accurately weighed 100 mg of a pulverized and uniform commercial Zhe Fritillaria sample, the sample treatment and other operations were the same as in Example 1, the sample was poured into a 1 cm cuvette through the membrane, and the absorbance measured at a wavelength of 354 nm was 308.315.

[0050] Calculate the content of sulfur dioxide in the sample:

[0051] ΔX = A - 87.03 517.32 X W X 10 6 = 308.315 - 87.03 517.32 X 100 X 10 6 = 4.28 X 10 3 mg

[0052] Five parallel determinations, the results are shown in Table 1;

Example Embodiment

[0053] Example 3

[0054] To determine the content of sulfur dioxide in Poria cocos, the steps are:

[0055] Accurately weighed 50 mg of a crushed and uniform commercially available Poria cocos sample. The sample treatment and other operations were the same as in Example 1. The sample was poured into a 1 cm cuvette through the membrane, and the absorbance measured at a wavelength of 354 nm was 262.846.

[0056] Calculate the content of sulfur dioxide in the sample:

[0057] ΔX = A - 87.03 517.32 X W X 10 6 = 262.846 - 87.03 517.32 X 100 X 10 6 = 6 . 80 X 10 3 mg

[0058] Five parallel determinations, the results are shown in Table 1;

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Abstract

The invention relates to an apparatus and a method for rapidly measuring the sulfur dioxide residue level in a Chinese herb, and belongs to the technical field of drug analysis. The method comprises: a, drawing a standard curve and deriving a calculation formula; b, directly detecting the Chinese herb without treatment; and c, calculating the result. The apparatus comprises a purified water bottle, a sodium hydroxide solution bottle, an o-phthalaldehyde solution bottle, an ammonium acetate solution bottle, a sodium sulfite solution bottle, a multi-channel micro-flow-controlled peristaltic pump, a reaction pool, an ultrasound instrument, a micro-flow-controlled peristaltic pump, a serpentine condensation pipe, a filtration membrane, a cuvette, a detector, and a workstation. The method has characteristics of simple equipment, simple operation, accurate detection result, and effective detection of sulfur dioxide in the Chinese herb.

Description

technical field [0001] The invention relates to the technical field of detection of sulfur dioxide in Chinese medicinal materials, in particular to a device and method for measuring sulfur dioxide in Chinese medicinal materials, belonging to the technical field of drug analysis. Background technique [0002] Sulfur dioxide residues in Chinese herbal medicines mainly exist in the form of free and bound states, and the free states include bisulfite (HSO 3 - ), SO 2 molecules and sulfites (SO 3 2- ), and easily form various forms of combined sulfur dioxide residues with reducing sugars, proteins, pigments, enzymes, vitamins, aldehydes, ketones, etc. in medicinal materials. Since irreversibly bound sulfite does not dissociate under physiological conditions and will not cause harm to human health, the detection objects of sulfur dioxide or sulfite are generally free sulfite and reversibly bound sulfite. [0003] Although the 2010 edition of "Chinese Pharmacopoeia" adds a met...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N1/38G01N21/64
Inventor 郑玉光马东来李菁康立英王靓吴枭吴迪温子帅
Owner HEBEI UNIV OF CHINESE MEDICINE
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