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Bacillus pumilus N103-1 and application thereof

A technology of Bacillus pumilus and biocontrol fungicides, applied in the direction of application, bacteria, fungicides, etc., to achieve the effects of reducing residues, human and animal safety, and enriching biocontrol resources

Active Publication Date: 2015-10-21
SUZHOU ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Bacillus pumilus (Bacillus pumilus) N103-1 is a new strain obtained by sampling and isolation by the applicant, which can effectively prevent and treat strawberry anthracnose caused by Colletotrichum fragariae. There is no relevant report at home and abroad, and it is creative and novel

Method used

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  • Bacillus pumilus N103-1 and application thereof
  • Bacillus pumilus N103-1 and application thereof
  • Bacillus pumilus N103-1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Inhibition of three pathogenic bacteria by Bacillus pumilus N103-1

[0024] A. The pathogenic bacteria tested

[0025] Pathogenic bacteria include Phytophthora amylovora, Phytophthora oryzae bacterial spot and Ralstonia solanacearum, provided by the Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences. Use LB culture solution (peptone 10.0g, yeast powder 5.0g, NaCl 10g, distilled water 1000mL) at 28°C, 150rpm shaking culture for 2 days, dilute to 10 with sterile water 8 cfu / mL, spare.

[0026] B. Test method:

[0027]The Bacillus pumilus N103-1 screened by Jiangsu Academy of Agricultural Sciences was activated on LB medium, then transferred into 50mL LB medium, cultured with shaking at 28°C and 150rpm for 2 days, and diluted with sterile water to 5×10 8 cfu / mL, spare.

[0028] Take 5 μL of Bacillus pumilus N103-1 bacteria solution, spot it on the center of LB plate, incubate at 28°C for 48 hours, spray with pathogenic bacteria diluent, incubate ov...

Embodiment 2

[0033] Inhibitory Effects of Bacillus pumilus N103-1 on Four Kinds of Phytopathogenic Fungi

[0034] A. For testing pathogenic fungi:

[0035] Pathogenic fungi include Strawberry Anthracnose, Tomato Fusarium, Rice Sheath Blight and Cabbage Black Spot, all provided by the Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences. Use PDA medium (200g potato, 20g glucose, 20g agar and 1000mL distilled water, pH 5.6-6.6) to culture at 28°C for 5d, set aside.

[0036] B. Test method:

[0037] The preparation of Bacillus pumilus N103-1 bacterial solution is the same as in Example 1.

[0038] Place the pathogenic fungus bacterium cake (6mm in diameter) on one side of the blank PDA plate, 30mm away from the edge of the plate. On the other side of the plate, 30 mm from the edge of the plate, 5 μL of Bacillus pumilus N103-1 bacterial solution was spotted. Cultivate at a constant temperature of 28°C, set up clean water as a control, and investigate the growth diameter ...

Embodiment 3

[0046] Metabolic secretions of Bacillus pumilus N103-1-production of antibacterial related substances

[0047] Test materials: protease detection medium: skimmed milk powder 100g, agar 20g, dilute to 1000mL. Cellulase detection medium: peptone 10g, yeast powder 10g, sodium carboxymethylcellulose 10g, NaCl 5g, KH 2 PO 4 1g, 20g agar, dilute to 1000mL, pH 7.0. Siderophilic detection medium: CAS 60.5mg, 10mL ferric iron solution (1mmol L -1 FeCl 3 ·6H 2 O), HDTMA 72.9mg, agar 20g, set to 1000mL, pH7.0.

[0048] The preparation of Bacillus pumilus N103-1 bacterial solution is the same as in Example 1. Spot 5 μL of Bacillus pumilus N103-1 bacterial solution in the center of each test medium plate, and repeat 3 times for each treatment. Incubate at a constant temperature of 28°C for 3-7 days and observe.

[0049] Protease detection: observe whether there is a transparent circle. If a transparent circle is produced, it indicates that the antagonistic bacteria can secrete pr...

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Abstract

The invention relates to a biological agent, wherein effective active components are composed of bacillus pumilus N103-1. The biological agent is characterized in that secreted metabolite comprises three antimicrobial-relative substances, namely, protease, cellulase and siderophore. The biological agent is an aqueous solution and comprises, by weight percentage, 98.8% of five hundred million bacilli pumilus N103-1, 1.0% of NNO and 0.2% of potassium sorbate. When strawberry seedlings are transplanted or planted, 1:50-500 times diluted solution of the bacillus pumilus N103-1 aqueous solution is sprayed on leaves of plants evenly for preventing and controlling strawberry anthracnose. The biological agent has the advantages that due to the fact that the bacillus pumilus N103-1 which is safe to people and livestock and has better preventive and therapeutic effect on multiple plant diseases is introduced, the biological agent is utilized to prevent and control the plate diseases; dosages of chemical agents are reduced, and the drug cost is reduced; meanwhile, the purposes of improving the ecological environment and reducing the amount of residues of the chemical agents in agricultural products are achieved.

Description

technical field [0001] The invention relates to a biocontrol agent and its application, in particular to a strain of bacillus pumilus N103-1 and its application in preventing and treating strawberry anthracnose. Background technique [0002] Strawberry anthracnose is one of the main diseases of strawberry. Generally, it can cause 10% to 20% yield reduction, and severe cases can reduce yield by more than 50%. It is more common in southern strawberry producing areas. Strawberry anthracnose mainly occurs in the seedling stage (stolon pumping stage) and the initial stage of planting, and it mainly damages stolons, petioles, leaves, stipules, petals, calyxes and fruits, and can cause local lesions or whole plants to wilt and die. The stolons, petioles, and leaves are infected, and initially produce black spindle-shaped or oval ulcer-like lesions with a diameter of 3-7 mm; when the lesions on the stolons and petioles expand into rings, the part above the lesions wilts and dies, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P3/00C12N1/20C12R1/07
Inventor 张青刘邮洲乔俊卿刘永锋
Owner SUZHOU ACADEMY OF AGRI SCI