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A method for detecting the content and authenticity of various organic acids in grape juice

A technology of grape juice and organic acid, applied in the field of analytical chemistry and food counterfeiting, to achieve the effect of small error, good precision and high detection accuracy

Active Publication Date: 2017-09-12
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But measure the content of multiple organic acids in grape juice at the same time, and the method for identifying the authenticity of grape juice based on this has not yet been reported in the literature

Method used

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  • A method for detecting the content and authenticity of various organic acids in grape juice
  • A method for detecting the content and authenticity of various organic acids in grape juice
  • A method for detecting the content and authenticity of various organic acids in grape juice

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Simultaneously detect the content of various organic acids in grape juice

[0025] a. Precisely weigh 10.0 mg each of tartaric acid, malic acid, ascorbic acid, lactic acid, acetic acid, citric acid, fumaric acid and succinic acid reference substance dried under reduced pressure to constant mass, dissolve in ultrapure water and dilute to 10 mL volumetric flask In, filter with 0.22 μm microporous membrane.

[0026] b. Take 5 mL of grape juice sample and add mobile phase to dilute to 25 mL, after ultrasonic mixing, centrifuge (9000 rpm / min, 10 min), take 1 mL of supernatant and filter through 0.22 μm microporous membrane to obtain the sample.

[0027] c. Analyze the samples prepared in a and b under the following conditions: Thermo Hypersil Gold aQ C18 column (250×4.6mm, 5 μm); mobile phase: methanol: ammonium dihydrogen phosphate buffer solution (0.02mol / L, pH 2.8)=1:99 (V / V); injection volume: 10 μL; flow rate: 0.8 mL / min; column temperature: 30° C.; detector: DAD; d...

Embodiment 2

[0054] 1. Establishment of fake grape juice identification model

[0055] Denote the organic acid content in pure grape juice as X1, X2, X3...Xn respectively, then the Logistic regression function is:

[0056] The π value of pure grape juice is recorded as 1, and the π value of non-prepackaged pure grape juice is recorded as 0, and the parameter fitting of the Logistic model is obtained: where x 1 、x 2 、x 3 、x 4 、x 5 、x 6 、x 7 Represents the contents of tartaric acid, malic acid, ascorbic acid, lactic acid, citric acid, fumaric acid and succinic acid respectively (unit: mg / mL).

[0057] 2. Validation of the Logistic model

[0058] 10 blind samples (including pure grape juice and grape juice drinks) purchased randomly in the market and self-made grape juice mixed with different volume ratios (low, medium, high) of apple juice were used as verification samples, and the organic acids in them were detected by HPLC. Obtain relevant data to calculate its π value to verif...

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Abstract

The present invention discloses a method for simultaneous determination of a variety of organic acids in grape juice. The method is as below: extracting a sample to be detected, and detecting by high performance liquid chromatography containing a DAD detector. the DAD detector has set detection wavelength of 210nm, the HPLC employs a reverse phase chromatographic column, a mobile phase of methanol and ammonium dihydrogen phosphate buffer solution with pH value 2.8 in the volume ratio of 1:99; and the reverse phase column has temperature of 30 DEG C, and a flow rate of 0.8mL / min. The invention also discloses a method for determination of authenticity of grape juice. The method can simultaneously determine the content of eight organic acids in grape juice, has high detection accuracy, good precision and small error, and reaches correct rate of 90% in the identification of authenticity of grape juice.

Description

technical field [0001] The invention belongs to the fields of analytical chemistry and food identification, and in particular relates to a method for determining the content of various organic acids in grape juice and a method for identifying authenticity of grape juice. Background technique [0002] The nutritional value of grapes is very high. In addition to containing a variety of organic acids, minerals, vitamins and essential amino acids, it also contains a variety of biologically active substances such as resveratrol, proanthocyanidins, anthocyanins, catechins and flavonoids. It has various physiological functions such as scavenging free radicals and antioxidant activity, protecting the heart, and improving immunity. Therefore, grape juice and grape juice products have become popular drinks in the world in recent years. Under the stimulation of huge market demand, the phenomenon of grape juice mixed with other fruit juices pretending to be pure grape juice is found fro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/89
Inventor 刘睿夏珺周密陈梁李宝丽
Owner HUAZHONG AGRI UNIV