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Anti-encephalitis B virus infection compositions and application thereof

一种乙型脑炎病毒、组合物的技术，应用在抗病毒剂、抵抗媒介传播的疾病、含有效成分的医用配制品等方向

Active Publication Date: 2015-11-11
SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although existing studies have proved that nitazoxanide can be used for the treatment of gastroenteritis, hepatitis C, and hepatitis B caused by rotavirus and norovirus (Norovirus), nitazoxanide has not been used in the treatment of Japanese encephalitis virus infection. see any reports

Method used

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  • Anti-encephalitis B virus infection compositions and application thereof
  • Anti-encephalitis B virus infection compositions and application thereof
  • Anti-encephalitis B virus infection compositions and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Example 1 The inhibitory effect of the mixed preparation of nitazoxanide and trehalose on the proliferation of Japanese encephalitis virus in BHK cells (purchased from the cell bank of Shanghai Institute of Biological Sciences, Chinese Academy of Sciences) (golden hamster kidney cells) in vitro

[0087] Implementation steps:

[0088] (1) Cell culture

[0089] BHK cells in good growth state were subcultured on a 6-well cell culture plate (Corning, USA) (1×10 6 cells / well). After 12 hours, wash with 1 ml of serum-free DMEM (GIBCO product of the United States, REF: 16000-044) medium, and add 1 × 10 cells to each well. 3 TCID 50 The Japanese encephalitis virus was placed in a cell culture incubator at 37°C for 1 hour. After taking out, wash twice with serum-free DMEM1ml, then add 3ml cell culture fluid containing 5 μ g / ml nitazoxanide and 5% trehalose (the cell culture fluid also contains 2% calf serum, 100 units of penicillin ( U.S. GIBCO company product, REF: 15140-1...

Embodiment 2

[0111] Example 2 Inhibition of the mixed preparation of nitazoxanide and trehalose on the proliferation of Japanese encephalitis virus in mice

[0112] Implementation steps:

[0113] (1) Animals: 90 female Kunming mice (Shanghai Slack Experimental Animal Co., Ltd.), weighing 12-14 g. Divided into 9 groups on average:

[0114] a. The drinking water of the poisoned mice contains 0.2% dimethyl sulfoxide (the percentage is the volume ratio of dimethyl sulfoxide / distilled water);

[0115] b. The drinking water of infected mice contained 100 μg / ml of nitazoxanide;

[0116] c. The drinking water of the infected mice contained 100 μg / ml of nitazoxanide and 2% trehalose;

[0117] d. The drinking water of the infected mice contained 100 μg / ml of nitazoxanide and 5% trehalose;

[0118] e. The drinking water of the infected mice contained 100 μg / ml of nitazoxanide and 8% trehalose;

[0119] f. The drinking water of the infected mice contained 100 μg / ml of nitazoxanide and 15% trehalo...

Embodiment 3

[0126] Example 3 Inhibition of the mixed preparation of nitazoxanide derivatives and trehalose on the proliferation of Japanese encephalitis virus in mice

[0127] The specific implementation steps are the same as in Example 2, and the test data are shown in Table 4.

[0128] According to Table 4, it can be seen that the nitazoxanide derivatives Tizoxanide, RM4803, RM4832, RM4848, RM4850, RM4851, RM4852, RM4863, RM4865 and RM5014 in the present invention can effectively reduce the mortality of mice infected with JE virus.

[0129] Table 4: Survival rate of inoculated mice after treatment with nitazoxanide derivatives

[0130]

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Abstract

The invention discloses compositions with anti-Japanese-encephalitis-virus functions, and applications thereof in preparing medicine used for preventing and / or treating Japanese encephalitis virus infections. The compositions comprise a N-(2-thiazolyl)benzamide compound represented by the formula A, and trehalose or Huangqi Shengmai Yin. R1 is OH, OCOCH3, OCOC2H5, OCOCH2CH2CH3 or OCOCH(CH3)2; R2, R3, and R4 are independently H or C1-C3 alkyl; R5 is halogen, nitro, or C1-C3 of the alkylsulfonyl; and R6 is H or C1-C3 alkylsulfonyl. The composition provided by the invention can effectively inhibit Japanese encephalitis virus proliferation.

Description

technical field [0001] The invention relates to a composition for resisting Japanese encephalitis virus infection and its application. Background technique [0002] Japanese Encephalitis (JE), also known as JE, is a viral encephalitis caused by Japanese encephalitis virus (JEV) infection. The disease is mainly transmitted by the bite of Culex mosquitoes. JE virus infection can cause severe neurological disorders and meningitis. About 1-3 of every 1000 JE virus infected patients are accompanied by neurological diseases, and the fatality rate of JE virus is about 30%. [0003] Every year, there are about 30,000-50,000 Japanese encephalitis clinical patients worldwide, and more than 10,000 deaths, and they are mainly distributed in China, Southeast Asia, India and other countries. At present, the epidemic range of Japanese encephalitis has expanded to South Asia, East Asia, Southeast Asia and the Asia Pacific Rim region. About 3 billion people in the world live in Japanese en...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7016A61K36/8968A61P31/14A61K31/426
CPCY02A50/30
Inventor 马志永魏建超史子学薛飞群李蓓蓓
Owner SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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