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A kind of preparation method and application of the flavonoid components of isoflavone

A technology of fennel flavonoids and fennel flavonoids, applied in medical preparations containing active ingredients, plant/algae/fungus/moss components, drug combinations, etc., can solve the problem that the active flavonoid components of hepatitis virus have not been reported in the literature, etc. , to achieve the effect of stable and controllable quality, simple process and good repeatability

Inactive Publication Date: 2019-12-06
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no report on the preparation of antiviral active flavonoids from P. heterophylla. Chinese patent (CN103070916B) reports that ethanol extraction and macroporous resin treatment are used to enrich flavonoids from P. heterophylla for the treatment of hypertension. However, So far, there has been no literature report on the preparation method of obtaining large-scale anti-hepatitis virus active flavonoids from Qinglan different leaves

Method used

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  • A kind of preparation method and application of the flavonoid components of isoflavone
  • A kind of preparation method and application of the flavonoid components of isoflavone

Examples

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Effect test

Embodiment 1

[0033] Example 1 A method for preparing the flavonoids of Indigo chinensis, comprising the following steps:

[0034] ⑴ extraction

[0035] After drying and crushing the plant of Cymbidium heterophyllum, add 8 times its mass in 95% ethanol solution and soak for 10 hours, heat and reflux at 80°C for 2 times, each time for 3 hours, to obtain the extract, which is subjected to Drying under reduced pressure until it is a paste is obtained to obtain the ethanol extract of the plant.

[0036] Wherein: the reduced-pressure drying condition refers to a vacuum degree of 0.09 MPa and a temperature of 50°C.

[0037] ⑵Liquid-liquid distribution and enrichment:

[0038] 1.0 Kg of the plant ethanol extract of Indigo isophyllum was dispersed with 30 times of its mass in analytically pure water to obtain a dispersion, which was extracted 3 times with 30 times of its volume of ethyl acetate, and combined to obtain an ethyl acetate extract; ethyl acetate The extract was dried under reduced pr...

Embodiment 2

[0050] Example 2 A method for preparing the flavonoids of Indigo chinensis, comprising the following steps:

[0051] ⑴ extraction

[0052] After drying and pulverizing the plant of Cymbidium heterophyllum, add 95% methanol solution with a volume concentration 20 times its mass and soak for 15 hours, heat and reflux extraction at 90°C for 4 times, each time for 3 hours, to obtain an extract, which is subjected to Drying under reduced pressure until it is a paste is obtained to obtain the ethanol extract of the plant.

[0053] Wherein: the reduced-pressure drying condition refers to a vacuum degree of 0.06 MPa and a temperature of 70°C.

[0054] ⑵Liquid-liquid distribution and enrichment:

[0055]5.0 Kg of the plant ethanol extract of Indigo isophyllum was dispersed with 50 times of its mass in analytically pure water to obtain a dispersion, which was extracted 3 times with 50 times of its volume of ethyl acetate, and combined to obtain an ethyl acetate extract; ethyl acetate ...

Embodiment 3

[0067] Example 3 A method for preparing the flavonoids of Indigo chinensis, comprising the following steps:

[0068] ⑴ extraction

[0069] After drying and pulverizing the plant of Cymbidium heterophyllum, add 14 times its mass in 95% ethanol solution and soak for 12 hours, heat and reflux extraction at 85°C for 3 times, each time for 3 hours, to obtain the extract, which is subjected to Drying under reduced pressure until it is a paste is obtained to obtain the ethanol extract of the plant.

[0070] Wherein: the reduced-pressure drying condition refers to a vacuum degree of 0.08 MPa and a temperature of 60°C.

[0071] ⑵Liquid-liquid distribution and enrichment:

[0072] 2.0 Kg of the plant ethanol extract of Indigo isophyllum is dispersed with 40 times of its mass in analytically pure water to obtain a dispersion, which is extracted 3 times with 40 times of its volume of ethyl acetate, and combined to obtain an ethyl acetate extract; ethyl acetate The extract was dried und...

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Abstract

The invention relates to a sample pretreatment method of flavonoid component of dracocephalum heterophyllum benth. The sample pretreatment method comprises the following steps: (1) carrying out liquid-liquid partitioning enrichment, namely, carrying out dispersion, extraction and vacuum drying on pohytol extract of dracocephalum heterophyllum benth to obtain medium polar component part of dracocephalum heterophyllum benth; (2) removing purities by adopting micro-reticular resin, namely, dissolving the medium polar component part of dracocephalum heterophyllum benth, filtering, separating by a micro-reticular resin column, eluting, collecting the eluate, and carrying out vacuum drying on the eluate to obtain the yellow flavonoid component of dracocephalum heterophyllum benth; (3) and carrying out chromatography isolation by adopting a silicagel column, namely, separating the flavonoid component crude product of dracocephalum heterophyllum benth by adopting the silicagel column chromatography, eluting, detecting by adopting thin-layer chromatography, mixing, and collecting fraction with the Rf value being 0.1-0.8, and carrying out vacuum drying on the fraction, thus obtaining the flavonoid component of dracocephalum heterophyllum benth, which is in the form of yellow powder. The technology is simple, and easy in realizing scale production. The invention further discloses an application of the flavonoid component of dracocephalum heterophyllum benth in preparing a drug or health food for resisting hepatitis virus.

Description

technical field [0001] The invention relates to a preparation method of a flavonoid component, in particular to a preparation method and application of a flavonoid component of isoflavone. Background technique [0002] different leaf green orchid ( Dracocephalum heterophyllum Benth) belongs to Labiatae ( Labiatae ) Cymbidium ( Dracocephalum ) plants, mainly distributed in temperate Asia. The plant is widely used in the folk, and has the effects of calming the liver, clearing away heat, and treating high blood pressure, lymphadenitis, and cough due to lung heat. Modern studies have shown that the chemical constituents in Indigo isophylla mainly include phenylpropanoids, flavonoids and terpenoids, among which flavonoids are well known for their significant antioxidant, cardiovascular and cerebrovascular disease, anti-tumor and anti-viral activities. [0003] At present, there is no report on the preparation of antiviral active flavonoids from P. heterophylla. Chinese pat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/53A61P31/12A61P1/16A23L33/105G01N30/90G01N30/06A23L33/00
Inventor 王启兰张莉党军陶燕铎王蒲于瑞涛梅丽娟郑威卢晓华
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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