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A kind of preparation method of dermal tissue bionic sponge

A tissue and dermis technology, applied in the field of medical biomimetic materials, can solve problems such as promoting functional dermal tissue regeneration, secondary surgery, virus transmission, etc., to promote adhesion and proliferation activity, and reduce biodegradation speed. , the effect of improving biocompatibility

Active Publication Date: 2018-06-05
WUXI NO 3 PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First of all, due to the difficulty in controlling the processing technology, the quality of acellular dermal matrix-like dermal biomimetic materials is unstable, and there are often quality differences between production batches. Serious consequences such as surgery
Secondly, there is a possibility of bio-contamination in the biomimetic material of acellular dermal matrix dermis, because the raw human cadaver skin may carry viruses, and these viruses are not effectively removed during material processing, resulting in the spread of viruses
Thirdly, the degradation rate of in vitro synthetic dermis bionic materials on the wound surface does not match the rate of tissue formation, and the effect of promoting wound repair and inhibiting scar formation is often weakened due to excessive degradation.
In addition, the effect of existing dermal bionic materials on inhibiting scar formation is far from achieving the goal of promoting the regeneration of functional dermis-like tissue

Method used

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  • A kind of preparation method of dermal tissue bionic sponge
  • A kind of preparation method of dermal tissue bionic sponge
  • A kind of preparation method of dermal tissue bionic sponge

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Put 10g of silkworm cocoons in 500ml of 0.02M Na 2 CO 3 Boiling in an aqueous solution for 30 minutes, then rinsing with deionized water for 3 times to obtain silk fibroin fibers; then placing silk fibroin fibers in 10ml of 9.3M lithium bromide solution and dissolving at 37°C to obtain silk protein lithium bromide solution; Finally, the obtained solution was dialyzed in a dialysis box with a molecular weight cut-off of 3500 Da for 3 days to obtain an aqueous silk protein solution, and the mass percentage concentration of the solution was adjusted to 5%;

[0027] (2) Mix the silk protein solution prepared in step (1) with the collagen solution with a mass percent concentration of 1.5% at a volume ratio of 7:10 at 37° C. Shake for 5 minutes until the mixture is uniform, then centrifuge for 3 minutes at a centrifugal force of 1500g to remove the air bubbles in the solution;

[0028] (3) The mixed solution after step (2) centrifugation is transferred in the polytetraf...

Embodiment 2

[0033] (1) Put 10g silkworm cocoons in 500ml of 1M Na 2 CO 3 Boiling in an aqueous solution for 30 minutes, then rinsing with deionized water for 3 times to obtain silk fibroin fibers; then placing silk fibroin fibers in 10ml of 10M lithium bromide solution and dissolving at 37°C to obtain silk protein lithium bromide solution; finally After dialysis of the obtained solution in a dialysis box with a molecular weight cut-off of 3500 Da for 3 days, an aqueous silk protein solution was obtained, and the mass percent concentration of the solution was adjusted to 5%;

[0034] (2) Mix the silk protein solution prepared in step (1) with the collagen solution with a mass percent concentration of 1.5% at a volume ratio of 57:10 at 37° C. Shake for 5 minutes until the mixture is uniform, then centrifuge for 3 minutes at a centrifugal force of 1500g to remove the air bubbles in the solution;

[0035] (3) The mixed solution after step (2) centrifugation is transferred in the polytetrafl...

Embodiment 3

[0039] (1) Put 10g silkworm cocoons in 500ml of 0.05M Na 2 CO 3 Boiling in an aqueous solution for 30 minutes, then rinsing with deionized water for 3 times to obtain silk fibroin fibers; then placing silk fibroin fibers in 10ml of 15M lithium bromide solution and dissolving at 37°C to obtain silk protein lithium bromide solution; finally After dialysis of the obtained solution in a dialysis box with a molecular weight cut-off of 3500 Da for 3 days, an aqueous silk protein solution was obtained, and the mass percent concentration of the solution was adjusted to 5%;

[0040] (2) Mix the silk protein solution prepared in step (1) with the collagen solution with a mass percent concentration of 1.5% at a volume ratio of 0.4:10 at 37° C. Shake for 5 minutes until the mixture is uniform, then centrifuge for 3 minutes at a centrifugal force of 1500g to remove the air bubbles in the solution;

[0041] (3) The mixed solution after step (2) centrifugation is transferred in the polytet...

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Abstract

The invention discloses a method for preparing a dermal tissue bionic sponge. The mass fraction of silk protein in the dermal tissue bionic sponge is 10% to 95%, and the mass fraction of collagen is 5% to 90%. The silk protein solution and collagen After the protein solution is mixed, the water vapor is evaporated to form a film, and then soaked in a mixed aqueous solution of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide and N-hydroxysuccinimide, and dried. Obtain the dermal tissue biomimetic sponge. The dermal tissue bionic sponge of the present invention has bionic performance on dermal tissue in terms of microstructure, mechanical properties and molecular structure, which is conducive to improving its performance of inhibiting scar formation and promoting functional dermal tissue regeneration, thereby providing a solution to the difficult problem of pathological scars. new method.

Description

technical field [0001] The invention relates to the technical field of medical bionic materials, in particular to a method for preparing a dermal tissue bionic sponge by using silk protein and collagen as raw materials. Background technique [0002] Skin defects caused by trauma such as burns, wounds, and surgical incisions are common clinical and frequently-occurring diseases. During the repair of skin defects, excessive wound healing will lead to the occurrence and development of pathological scars. Pathological scars include hypertrophic scars and keloids, which can cause a series of serious complications such as itching, disfigurement, and disability, and sometimes even induce malignant tumors, seriously affecting the quality of life of patients. Therefore, the prevention and treatment of pathological scars has always been a difficult problem to be solved in the fields of surgery such as burns and plastic surgery. [0003] In recent years, with the continuous deepening ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/26A61L27/56
Inventor 赵朋吕国忠
Owner WUXI NO 3 PEOPLES HOSPITAL
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