Method for extracting dihydroquercetin from larix gmelini wood powder

A dihydroquercetin and extraction method technology, which is applied in the field of dihydroquercetin extraction, can solve the problems of volatilization loss, high temperature, and system opening, and achieve the effects of reducing environmental pollution, increasing yield, and accelerating dissolution

Inactive Publication Date: 2015-11-11
NORTHEAST FORESTRY UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These traditional extraction processes have certain disadvantages. Compared with these traditional extraction processes, the "Study on Ultrasonic-Assisted Extraction of Dihydroquercetin from Larch Wood Powder by Ionic Liquid-Lithium Salt Microwave Pretreatment" shows that t

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Accurately weigh 1g of larch wood powder, add to different kinds of ionic liquid [C4min]Br, [C4min]Cl, [Bzmin]Br (the concentration of ionic liquid is 0.01mol) different kinds of lithium salt lithium hydroxide, lithium acetate , lithium nitrate, lithium chloride anhydrous, lithium bromide, lithium perchlorate (the consumption of lithium salt is 66mg), join in the dry 100ml flat-bottomed flask, add the acetonitrile of 10ml, stir rapidly, make it fully dissolve. Stir quickly, and quickly use a rotary evaporator to reduce pressure at about 70°C to evaporate all the organic solvents that dilute the ionic liquid. Microwave pretreatment was further carried out. The microwave temperature was 100° C. and the microwave time was 2 min. Then, 20 ml of deionized water was added for 250 W ultrasonic extraction for 30 min. 1 ml of the filtrate was filtered and centrifuged, and determined by HPLC.

Embodiment 2

[0022] Accurately weigh 1g of larch wood powder, add ionic liquid [C4min]Br (the concentration of ionic liquid is 0.006mol) and lithium perchlorate (the consumption of lithium salt is 44mg) and join in the dry 100ml flat-bottomed flask, add 10ml of acetonitrile , stir rapidly to make it fully dissolved. Stir quickly, quickly use a rotary evaporator to depressurize at about 70°C to evaporate all the organic solvents that dilute the ionic liquid, and then perform microwave pretreatment. The microwave temperature is 100°C and the microwave time is 2min. Then add 20ml deionized water and 250W ultrasonic extraction After 30 minutes, 1ml of the filtrate was collected and centrifuged, and determined by HPLC.

Embodiment 3

[0024] Accurately weigh 1g of larch wood powder, add ionic liquid [C4min]Br (the concentration of ionic liquid is 0.014mol) and lithium perchlorate (the consumption of lithium salt is 88mg) and join in the dry 100ml flat-bottomed flask, add 10ml of acetonitrile , stir rapidly to make it fully dissolved. Stir quickly, quickly use a rotary evaporator to depressurize at about 70°C to evaporate all the organic solvents that dilute the ionic liquid, and then perform microwave pretreatment. The microwave temperature is 100°C and the microwave time is 2min. Then add 20ml deionized water and 250W ultrasonic extraction After 30 minutes, 1ml of the filtrate was collected and centrifuged, and determined by HPLC.

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Abstract

The invention aims to carry out microwave pre-treatment by utilizing ionic liquid lithium salt before ultrasonic extraction, so as to dissolve part of a plant cell wall, enhance cellular permeability, accelerate solvent permeation, improve solvent function efficiency, and achieve the purpose of improving the extraction efficiency of dihydroquercetin. The method can remarkably improve dihydroquercetin yield, and is characterized by comprising the following steps: step 1, completely drying part of larch hadromestome, and smashing with a pulverizer; step 2, dissolving with a ionic liquid; step 3, adding larch wood powder obtained in the step 1 into the liquid obtained in the step 2, stirring fast to obtain a uniform liquid, and completely steaming out an organic solvent diluting ionic liquid lithium salt with a rotary evaporator; step 4, adopting microwave to further pretreat the product obtained in the step 3; step 5, further adding deionized water into the pretreated product after the pretreatment is finished, and carrying out ultrasonic auxiliary extraction; step 6, filtering, taking 1 mL filtrate for centrifugation, and carrying out HPLC detection.

Description

technical field [0001] The invention relates to the field of natural product chemistry, in particular to a method for extracting dihydroquercetin from larch wood powder. Background technique [0002] Dihydroquercetin, also known as Taxifolin and Taxifolin, is a bioflavonoid. As a powerful antioxidant, dihydroquercetin is widely used. Dihydroquercetin can effectively eliminate excess free radicals from the human body, promote the permeability of capillaries, improve immune function, reduce the formation of cancer, and prevent cardiovascular diseases. It restores the elasticity of capillaries, prevents inflammation and The formation of lumps. At the same time, it is also a high natural food antioxidant, and the antioxidant effect of dihydroquercetin exceeds that of other antioxidants such as quercetin, rutin and synthetic antioxidants. Adding dihydroquercetin to vegetable oil, animal fat, dry milk powder, and fat-containing confectionery can extend its validity period by 2 ...

Claims

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Application Information

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IPC IPC(8): C07D311/32
CPCC07D311/32
Inventor 杨磊祖元刚赵连飞刘再枝杜新琦韩峰李双阳
Owner NORTHEAST FORESTRY UNIVERSITY
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