Method for improving fermentation efficiency of anaerobic qi-eating microorganisms

A microbial fermentation and microbial technology, applied in the direction of fermentation, can solve the problems of low growth concentration, hinder the application of microbial fermentation technology, etc., and achieve the effect of improving biomass accumulation

Active Publication Date: 2015-11-11
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, the low growth concentration of aerobic anaerobic bacteria in the fermentation process inhibits their gas utilization efficienc

Method used

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  • Method for improving fermentation efficiency of anaerobic qi-eating microorganisms
  • Method for improving fermentation efficiency of anaerobic qi-eating microorganisms
  • Method for improving fermentation efficiency of anaerobic qi-eating microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Use a hollow cellulose membrane to intercept and backflush the plateau fermentation broth: add 20ml of mineralsolutionstock, 0.5g of yeast extract, 5g of morpholineethanesulfonic acid, and 50ml of reducingagentStock to a 1L fermentation system, and extinguish at 121 degrees Bacteria 20min. After sterilization, cool to about 70 degrees, then add 1ml of trace element storage solution, replace the gas in the fermenter and fresh medium storage tank with nitrogen with a purity of 99.999%, and control the rate of nitrogen introduction at 500ml / min. When the temperature of the fermenter drops to about 37 degrees Celsius, install the heating jacket and stirring controller, connect the hollow cellulose membrane module, fermenter and fresh medium storage tank with a pressure-resistant hose, and turn off the nitrogen connected to the fermenter. Always maintain nitrogen gas connected to the fresh media storage tank. Feed simulated syngas (gas composition: 50% CO, 35% CO2, 15% H2) ...

Embodiment 2

[0030] Retention backflushing of post-exponential broth using hollow cellulose membranes:

[0031] The culture medium preparation method and steps, device connection method and operation steps are the same as in Example 1, the difference is that the time point of cell interception is advanced to the post-exponential growth stage of the bacterial growth state.

[0032] Effect detection:

[0033]When the hollow cellulose membrane was used to intercept the late-exponential fermentation broth, and after washing and re-fermentation with fresh medium, first, the effective fermentation period of bacteria was also extended to 10 days, but the highest cell concentration reached 4.7Abs , is 3.9 times that of when the hollow cellulose membrane is not used, and the ethanol production is up to 1.6g / L, which is 2.7 times higher than that without the hollow cellulose membrane; the butanol production is up to 0.9g / L, which is comparable to Compared with the entrapment re-fermentation in the ...

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Abstract

The invention aims at providing a method for improving fermentation efficiency of anaerobic qi-eating microorganisms. The method for improving the fermentation efficiency of the anaerobic qi-eating microorganisms comprises the following steps: firstly carrying out filtration interception on an anaerobic qi-eating microorganism bacterial liquid in a fermentation system by virtue of a hollow cellulose membrane, then carrying out back flushing by virtue of a culture medium, and carrying out fermentation on intercepted thalli again. The method for improving the fermentation efficiency of the anaerobic qi-eating microorganisms has the advantages that interconnection between a fermentation tank and a hollow cellulose membrane component is realized in a gaseous fermentation process of the anaerobic qi-eating microorganisms, so that fermentation liquor circulates between the inner cavity of the hollow cellulose membrane and the fermentation tank; meanwhile, a peristaltic pump connected with the outer wall of the hollow cellulose membrane component is used for pumping and drainage, realizing suction filtration on the fermentation liquor and intercepting the thalli, then a fresh culture medium is used for washing the thalli adhered in the cavity of the hollow cellulose membrane into the fermentation tank, and repeated and continuous fermentation is carried out; and the method for improving the fermentation efficiency of the anaerobic qi-eating microorganisms can be used for improving biomass accumulation of anaerobic qi-eating clostridia in the gaseous fermentation process and effectively improving yield of ethyl alcohol, butyl alcohol and hexyl alcohol which are produced by utilizing synthesis gas.

Description

technical field [0001] The invention belongs to the technical field of anaerobic microorganism gas fermentation, and in particular relates to a method for improving the fermentation efficiency of anaerobic microorganism gas. technical background [0002] Syngas is a gas that mainly contains CO, H 2 and a small amount of CO 2 The mixed gas of other components, such as coal, oil, natural gas, industrial waste gas (such as coke oven gas, refinery gas), municipal solid waste and biomass, etc. There is a class of anaerobic gas-eating microorganisms in nature, which can produce syngas (CO, CO 2 and H 2 ) grows as the only carbon and energy source, and converts it into a variety of organic acids and alcohols, including high value-added chemicals such as acetic acid, butyric acid, 2,3-butanediol and ethanol, n-butane Alcohol, n-hexanol and other biofuels. At present, the excessive use of fossil energy and the burning of urban waste and straw have caused CO, CO 2 、CH 4 The ris...

Claims

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Application Information

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IPC IPC(8): C12P1/04
Inventor 谢彬涛韩一凡郭亚琼王兴彪王敬敬张小霞黄志勇
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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