Fatty-liver-related liver cancer model building method based on knockout mice

Abstract

The invention relates to a fatty-liver-related liver cancer model building method based on knockout mice. The method is characterized in that APOE- / - and LDLR- / - knockout mice including female mice and male mice are selected, the female mice and the male mice are matched in number, and double knockout mice are obtained; a primer is designed, and the genotypes in mice are identified through a PCR (polymerase chain reaction) system; the PCR (polymerase chain reaction) system comprises an ApoE gene identification PCR conditions and LDLR gene identification PCR conditions; the double knockout mice are pregnant, and suckling mice are obtained; streptozotocin is injected to the suckling mice, the suckling mice begin a high-fat diet after ablactation, and finally the suckling mice surfer from liver cancer; it is identified that the suckling mice begin the high-fat diet after ablactation, fatty liver occurs six weeks later, NASH occurs 8-10 weeks later, dysplastic nodule occurs 13-16 weeks later, and the liver cancer occurs 18-24 weeks later. The occurrence rate of the liver cancer is about 100% 24 weeks later. The time when pure-gene APOE- / - and LDLR- / - knockout mice suffer from NASH and HCC due to the high-fat diet is advanced by 24-30 weeks, and the occurrence rate is high.

Description

technical field [0001] The invention belongs to the field of life science and biotechnology, in particular to a method for constructing a fatty liver-related liver cancer model based on gene knockout mice, which is used to simulate the occurrence and development of human non-alcoholic steatohepatitis (NASH)-related liver cancer (HCC) process. Background technique [0002] HCC is the fifth most common malignancy and the third leading cause of cancer-related deaths in the world. According to statistics, about 110,000 people die of liver cancer every year in my country, accounting for about 45% of the world's liver cancer deaths. Studies have shown that the incidence of liver cancer related to non-alcoholic fatty liver disease (NAFLD) is 2.6%, which is equivalent to the incidence of liver cancer caused by hepatitis C (4%), and has gradually become the main cause of HCC in developed countries such as Europe and the United States. The pathogenic mechanism is not yet clear. [...

AI Technical Summary

Problems solved by technology

In recent years, carcinogen-induced liver cancer models, tumor transplantation models, and viral liver cancer models have all been well studied, but these models cannot replicate the human process from simple fatty liver, steatohepatitis, liver fibrosis to liver cancer

However, NASH-related HCC models are complicated to model and take a long time to model, so there are few studies

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