Fatty-liver-related liver cancer model building method based on knockout mice

Abstract

The invention relates to a fatty-liver-related liver cancer model building method based on knockout mice. The method is characterized in that APOE-/- and LDLR-/- knockout mice including female mice and male mice are selected, the female mice and the male mice are matched in number, and double knockout mice are obtained; a primer is designed, and the genotypes in mice are identified through a PCR (polymerase chain reaction) system; the PCR (polymerase chain reaction) system comprises an ApoE gene identification PCR conditions and LDLR gene identification PCR conditions; the double knockout mice are pregnant, and suckling mice are obtained; streptozotocin is injected to the suckling mice, the suckling mice begin a high-fat diet after ablactation, and finally the suckling mice surfer from liver cancer; it is identified that the suckling mice begin the high-fat diet after ablactation, fatty liver occurs six weeks later, NASH occurs 8-10 weeks later, dysplastic nodule occurs 13-16 weeks later, and the liver cancer occurs 18-24 weeks later. The occurrence rate of the liver cancer is about 100% 24 weeks later. The time when pure-gene APOE-/- and LDLR-/- knockout mice suffer from NASH and HCC due to the high-fat diet is advanced by 24-30 weeks, and the occurrence rate is high.

Description

technical field [0001] The invention belongs to the field of life science and biotechnology, in particular to a method for constructing a fatty liver-related liver cancer model based on gene knockout mice, which is used to simulate the occurrence and development of human non-alcoholic steatohepatitis (NASH)-related liver cancer (HCC) process. Background technique [0002] HCC is the fifth most common malignancy and the third leading cause of cancer-related deaths in the world. According to statistics, about 110,000 people die of liver cancer every year in my country, accounting for about 45% of the world's liver cancer deaths. Studies have shown that the incidence of liver cancer related to non-alcoholic fatty liver disease (NAFLD) is 2.6%, which is equivalent to the incidence of liver cancer caused by hepatitis C (4%), and has gradually become the main cause of HCC in developed countries such as Europe and the United States. The pathogenic mechanism is not yet clear. [...

AI Technical Summary

Problems solved by technology

In recent years, carcinogen-induced liver cancer models, tumor transplantation models, and viral liver cancer models have all been well studied, but these models cannot replicate the human pr

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