Bacterial complex for high-temperature composting of municipal solid wastes and production method thereof

A technology for municipal solid waste and compound inoculum, applied in the field of compound microbial inoculants, can solve the problems of low degradation rate of complex compounds, long composting cycle, poor competitiveness, etc., to shorten the composting cycle, improve compost quality, and stabilize the effect. Effect

Inactive Publication Date: 2015-11-25
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention aims at the disadvantages of poor competitiveness of existing commercially available bacterial agents, long composting period, and low degradation rate of complex compounds such as lignocellulose in compost materials, and provides a composite bacterial agent for high-temperature composting of municipal solid waste and a production method thereof. The composite microbial agent has the characteristics of multi-bacteria symbiosis, complementary enzyme systems, and comprehensive functions. It can effectively degrade complex components such as cellulose, hemicellulose, and lignin, realize rapid decomposition, improve compost productivity, and improve compost quality. It is economically possible. sex

Method used

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  • Bacterial complex for high-temperature composting of municipal solid wastes and production method thereof
  • Bacterial complex for high-temperature composting of municipal solid wastes and production method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1: the preparation of composite bacterial agent

[0025](1) Incline culture of single strains: under sterile conditions, inoculate Clostridium thermocellum, Thermotoga maritima, Bacillus subtilis, Bacillus stearothermophilus, Bacillus thermotolerant and actinomycetes on LB solid On the culture medium, culture in a constant temperature incubator at 60°C for 2 days;

[0026] (2) Primary seed cultivation: under sterile conditions, inoculate the strains cultivated in step (1) into LB liquid medium respectively, and vibrate at 200 rpm at 60°C for 2-5 days to obtain primary seeds;

[0027] (3) Expanded cultivation of secondary seeds: under aseptic conditions, the primary seeds prepared in step (2) are respectively expanded and cultivated at an inoculum size of 5-10% (v / v), and cultured by shaking at 200 rpm under 60°C for 3 -6d, to produce high-density cultured secondary seeds.

[0028] (4) Bacterial agent preparation: dehydrate and dry various secondary single b...

Embodiment 2

[0029] Example 2 Degradation experiment of triangular flask garbage using meal waste and corn stalks as main raw materials

[0030] 100g of sorted meal waste, 100g of corn stalk scraps, after mixing, adjust the moisture to make the moisture content of the material 55%, add 1g of compound high-temperature bacteria agent and stir evenly, heat up to 65°C and run for 3 days. The results showed that within 3 days, the soybean products and meat products in the food waste were rapidly degraded after adding the high-temperature compound bacterial agent, among which the crude fiber, crude fat and crude protein were degraded by 11.5%, 15.6% and 40.0% respectively. The contents of the three in the high-temperature compound bacterial agent group were almost unchanged. The fermentation temperature of the high-temperature composite bacterial agent group was always maintained at 55-65°C, while the fermentation temperature of the non-inoculated group gradually decreased to about 30°C with tim...

Embodiment 3

[0031] Embodiment 3 is the compost of main raw material with municipal solid waste

[0032] Firstly, the raw garbage is manually sorted, and organic solids such as cartons, plastic products, metals, and stones are removed to obtain organic domestic waste, which mainly includes vegetable and fruit waste, kitchen waste, waste paper, and garden waste. Add 0.5% high-temperature composite microbial agent, add water to adjust the moisture content of the compost material to about 50%, and compost after fully stirring with a mixer. Turn over every 3-4 days depending on the change of fermentation temperature. The leachate produced during the fermentation process is refilled into the compost, and the compost is fermented at 65°C for 10-15 days to mature. It was measured that the organic matter content decreased by 58.2%, the cellulose content decreased by 40.0%, the hemicellulose content decreased by 55.4%, and the lignin content decreased by 32.2%.

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Abstract

The invention provides a bacterial complex for high-temperature composting of municipal solid wastes and a production method thereof. The bacterial complex comprises Clostridium thermocellum, Thermotoga maritima, Bacillus subtilis, Bacillus stearothermophilus, thermophilic Bacillus, and Thermoactinomyces and is prepared by single-bacterium slant culture, enlarged culture with seed solution, and drying and mixing. Biomass and bacterial ratio of the bacterial complex are optimized, and the number of effective viable cells is up to 2.0*109cfu / mL. When the bacterial complex is inoculated for composting, fermentation temperature is kept between 55 DEG C and 65 DEG C, and composting period is shortened to 10 to 15 days. The bacterial complex has the advantages that fermentation temperature rise is fast, composting temperature is kept high, complex components such as lignocellulose are degraded at high rate, and composting period is short; waste composting can be significantly promoted, the proportion of effective nutrients is increased, compost quality is improved, and therefore the bacterial complex is widely applicable to high-temperature composting of various municipal solid wastes.

Description

technical field [0001] The invention relates to the technical field of bioengineering microbial fermentation, in particular to a composite microbial bacterial agent used for high-temperature composting of urban domestic waste. The invention also relates to a production method of the composite microbial agent and its application in high-temperature composting of urban domestic garbage. Background technique: [0002] Municipal solid waste refers to the waste generated by residents' daily consumption and daily office work. According to different sources and properties of garbage, it mainly includes general garbage, kitchen garbage, cleaning garbage, etc. In recent years, due to the gradual acceleration of urbanization, the increase of population, and the expansion of city scale, the average amount of urban domestic waste in my country has continued to increase at a rate of 8.98%. More than 100 million square meters. These garbage not only occupy a large amount of land and des...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/00C05F11/08C05F9/00C05F3/02C12R1/145C12R1/01C12R1/125C12R1/07
CPCY02A40/20Y02W30/40
Inventor 韩业君贾晓静彭小伟乔玮博
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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