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Stem cell serum-free culture medium and stem cell culture method

A serum-free medium and stem cell technology, applied in the field of stem cell serum-free medium and stem cell culture, can solve problems such as pathogen contamination, achieve the effects of clear ingredients, avoid cytotoxic effects, and improve repeatability

Inactive Publication Date: 2015-11-25
SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a non-toxic and pollution-free serum-free culture medium for stem cells in view of the defects of certain toxic effects and pathogen contamination in the prior art of using animal serum to cultivate stem cells

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  • Stem cell serum-free culture medium and stem cell culture method

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Embodiment 1

[0039] In this embodiment, the components of the stem cell serum-free medium and the concentration of each component in the stem cell serum-free medium are respectively:

[0040] DMEM / F12 medium: 100mL;

[0041] Sodium selenite: 50μg / ml;

[0042] Transferrin: 150μg / ml;

[0043] Ferric citrate: 50μg / ml;

[0044] Insulin: 15μg / ml;

[0045] The concentration of glutamine is 5mmol / l;

[0046] The concentration of progesterone is 10mmol / l;

[0047] The concentration of butanediamine was 40 μmol / l.

Embodiment 2

[0049] In this embodiment, the components of the stem cell serum-free medium and the concentration of each component in the stem cell serum-free medium are respectively:

[0050] DMEM / F12 medium: 500mL;

[0051] Sodium selenite: 40μg / ml;

[0052] Transferrin: 100μg / ml;

[0053] Insulin: 7μg / ml;

[0054] The concentration of glutamine is 3mmol / l;

[0055] The concentration of HEPES is 10mmol / l;

[0056] The concentration of progesterone is 15mmol / l;

[0057] The concentration of butanediamine is 60 μmol / l;

[0058] The concentration of penicillin was 60 mg / ml.

Embodiment 3

[0060] In this embodiment, the components of the stem cell serum-free medium and the concentration of each component in the stem cell serum-free medium are respectively:

[0061] Sodium selenite: 30μg / ml;

[0062] Transferrin: 100μg / ml;

[0063] Insulin: 2.5μg / ml;

[0064] The concentration of glutamine is 2mmol / l;

[0065] The concentration of HEPES is 5mmol / l;

[0066] The concentration of progesterone is 20mmol / l;

[0067] The concentration of butanediamine is 60 μmol / l;

[0068] The concentration of penicillin is 50mg / ml;

[0069] The mass fraction of glucose is 0.6%;

[0070] NaHCO 3 The concentration is 3mmol / l;

[0071] The mass fraction of soybean trypsin inhibitor is 0.1%;

[0072] Linoleic acid 60μmol / l;

[0073] The concentration of streptomycin was 50 mg / ml.

[0074] In order to further verify that the serum-free medium for stem cells provided by the present invention has outstanding and significant effects, specific experiments will be carried out in de...

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Abstract

The invention relates to a stem cell serum-free culture medium. The stem cell serum-free culture medium comprises a basal culture medium, insulin, glutamine, ferruginous food additives, sodium selenite, progestone and putrescine. The invention also discloses a stem cell culture method. The stem cell culture method comprises the following steps of acquiring stem cells; and culturing the stem cells in vitro by using the stem cell serum-free culture medium. Through cells obtained by the stem cell serum-free culture medium, the problem of cell purification difficulty caused by pollution of pathogens carried by a serum culture medium can be avoided, stability of cell production can be improved, cell proliferation quantity is increased, and cell viability is improved.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a serum-free medium for stem cells and a culture method for stem cells. Background technique [0002] At present, a certain proportion of animal serum is added to the conventional culture system of stem cells, and fetal bovine serum or newborn calf serum is more commonly used. Serum is an extremely complex mixture composed of many biomolecules of different sizes. Its main role in culturing cells in vitro is to provide growth factors, hormones, binding proteins, and provide protection. But it also contains some inhibitory factors or toxic substances that are not conducive to cell growth, and have potential cytotoxic effects. A large number of complex proteins in serum bring difficulties to the standardization of cell culture, and also to the separation and purification of cell culture expression products. Moreover, animal serum may contain known or unknown pathogens c...

Claims

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Application Information

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IPC IPC(8): C12N5/0775C12N5/074C12N5/0735C12N5/0789C12N5/0797
Inventor 曾宪卓鲁菲
Owner SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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