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A kind of hypocrellin B nanoparticle and preparation method thereof

A kind of Bamboo Rhododendron B, nanoparticle technology, applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, wave energy or particle radiation treatment materials, etc. It can solve problems such as direction transfer, and achieve the effect of good dispersibility, uniform morphological rules, and improved water solubility.

Active Publication Date: 2018-01-30
SHANDONG UNIV QILU HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The Chinese patent "Preparation Method of Water-Soluble Hypocretin Titanium Dioxide Nanoparticles" has developed a titanium dioxide-based hypocretin nanoparticle that improves stability and singlet oxygen yield, but the titanium dioxide used in the patent is in After intravenous injection into the human body, it is difficult to be completely metabolized and eliminated by the human body, and there are certain safety hazards
However, the nanoparticles prepared by this patent are mainly passively targeted in vivo, and it is difficult to achieve active targeted delivery of drugs

Method used

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  • A kind of hypocrellin B nanoparticle and preparation method thereof
  • A kind of hypocrellin B nanoparticle and preparation method thereof
  • A kind of hypocrellin B nanoparticle and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: Optimization of preparation conditions of Bamboo Rhododendron B nanoparticles

[0037] 1. Investigation of the preparation method

[0038] Nanoparticles were prepared by rotary evaporation method, dialysis method and rotary evaporation dialysis method, respectively. In rotary evaporation method, the solution of drug and nanomaterial was added dropwise to water, and the micelle solution was obtained by stirring at a certain rotation speed. In the dialysis method, drugs and nanomaterials are directly packed in a dialysis bag, and a micellar solution is obtained by dialysis. The rotary dialysis evaporation method combines the two. The rotary evaporation method is the same as the above. The rotary evaporated solution is put into a dialysis bag and dialyzed in water to obtain a micelle solution.

[0039] The nanoparticles prepared by different methods were observed by transmission electron microscope, and the results are shown in Table 1.

[0040] Table 1 Eff...

Embodiment 2

[0058] Example 2: Preparation of blank nanoparticles

[0059] After accurately weighing the PEG-PLA 30 mg mixture, it was dissolved in 2 ml of organic solvent acetone or tetrahydrofuran to prepare 15 mg / ml of organic solvent. In another 50ml beaker, add 20ml of ultrapure water, under the condition of magnetic stirring at a certain speed (magnetic stirrer 2, about 660rpm / min), use a 2ml injection needle to inject the organic solution containing nanomaterials with 20- The speed of 30 drops / min was dropped into pure water drop by drop, after continuing to stir for 2 hours, let stand at 4 °C overnight, then put the liquid into a dialysis bag (molecular weight cut-off 3500kDa), and then put it into a beaker filled with 1L ultrapure water During dialysis, the water was changed at 1h, 5h, 8h, 12h, 24h, 48h and 72h, and the organic solution was dialyzed out to obtain a milky white micelle solution.

[0060] The TEM images of the blank nanoparticles prepared in this example are shown ...

Embodiment 3

[0061] Example 3: Preparation of Bamboo Rhododendron B nanoparticles

[0062] Weigh the polymer PEG-PLA 27 mg, and weigh the polymer folate-PEG-PLA 3 mg, so that the ratio of the polymer with folic acid accounts for 10% of the total polymer, and accurately weigh the bamboo rhodopsin B (HB) under dark conditions. 1mg. Dissolve accurately weighed HB and high molecular polymer with 1ml and 1ml of organic solvent tetrahydrofuran respectively, shake until completely dissolved, and then mix the two. Ultrasound for 2-3min under the ultrasonic power of 30-40W to make the drug organic solution and the polymer organic solution evenly mixed, so as to prepare a drug polymer organic solution with uniform concentration. Add 20ml of water to a 50ml beaker, and drop the organic solution into pure water drop by drop at a speed of 25 drops / min under the condition of a certain magnetic stirring speed (Magnetic Stirrer 2, about 660 rpm / min). After finishing, stirring was continued for 2 h to pr...

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Abstract

The invention discloses a preparation method of Bamboo Rhododendron B nanoparticles. The steps are as follows: (1) dissolving a carrier polymer with an organic solvent to prepare a solution A with a concentration of 10-20 mg / ml; (2) dissolving bamboo The rubricin B is dissolved in an organic solvent to make a solution B with a concentration of 1-2mg / ml; (3) solution A and solution B are mixed by volume ratio of (1-14): 1 to make a solution with a uniform concentration Drug polymer organic solution; (4) under stirring condition, the drug polymer organic solution prepared in step (3) is added dropwise to water, the volume ratio of drug polymer organic solution and water is 1:(10-30), static Set for 12-24h to prepare a micellar solution; (5) transfer the micellar solution to a dialysis bag, place the dialysis bag in water for dialysis, and dialyze the organic solution to obtain rhodopsin B nanoparticles. The nanoparticles prepared by the invention are observed by transmission electron microscope, and the shape is spherical, regular, good in dispersibility, uniform and non-adhesive.

Description

technical field [0001] The present invention relates to a kind of Bamboo Rhododendron B nanoparticles and a preparation method thereof. Background technique [0002] Photodynamic therapy (PDT) is a promising treatment method. It refers to a treatment method in which a photosensitive chemical reaction is caused by irradiating a photosensitizer with a sensitizing light source to generate reactive oxygen species, thereby destroying the diseased tissue. The photosensitizer itself can selectively accumulate in the diseased tissue, and the irradiation light source can be limited to the diseased part during treatment to achieve dual selection. The advantages of PDT are selective killing of diseased tissue, low toxicity, repeatable application, and can also be combined with surgery and other treatments. The application of PDT to the treatment of tumors is also a current hotspot. [0003] Photosensitizers are an important part of photodynamic therapy. Bamboo Rhododendron B is the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K9/14A61K47/22A61P35/00
Inventor 宋坤孔北华鲁再君李钊苑存忠
Owner SHANDONG UNIV QILU HOSPITAL
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