Ginseng fermentation puree as well as preparation method and application thereof
A technology of fermenting puree and ginseng, which is applied in the biological field, can solve problems such as damaging the environment, and achieve the effects of simplifying production steps, saving production costs, and being easy to absorb
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Embodiment 1
[0031] Embodiment 1, a kind of ginseng fermented protoplasm and preparation method thereof:
[0032] 1. Activation of strains:
[0033] Picking a ring of Bifidobacterium bifidum colonies in the liquid culture medium, putting them into a shaker to activate the strains, and obtaining the activated Bifidobacterium bifidum bacteria liquid.
[0034] 2. Purification of strains:
[0035] The activated bacterial solution obtained in step 1 was serially diluted and plated to obtain a single colony to obtain purified Bifidobacterium bifidum.
[0036] 3. Expansion and cultivation of strains:
[0037] Inoculate the purified bacterial classification obtained in step 2 into the YPD medium (pH value is 7.0), and cultivate it in a shaker at 25° C. In the logarithmic phase, the concentration is 10 6 -10 9 CFU / ml).
[0038] 4. Obtaining ginseng fermented puree:
[0039] 20mL of the fermented bacteria liquid that step 3 obtains (concentration is 10 9 CFU / ml) was inoculated into 10 g of g...
Embodiment 2
[0043] Embodiment 2, the application of ginseng fermented protoplasm as cosmetics (mask or essence or toner):
[0044] 1. Safety testing of ginseng fermented puree:
[0045] The human patch test is mainly used to detect the irritation of cosmetic final products or raw materials. The present invention conducts closed patch test on human body on the fermented ginseng puree obtained in Example 1, aiming at evaluating its potential skin irritation.
[0046] 1. Test object:
[0047] Choose 30 suitable volunteers, randomly selected in the age range of 18-60 years old.
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