Preparation of fructus forsythiae active site and application of fructus forsythiae active site to Parkinson disease

An active site, Parkinson's disease technology, applied in the field of medicine, can solve the problem of no PC12 cells, etc., and achieve the effect of improving PD-like behavioral changes, reducing dosage, and increasing the number

Active Publication Date: 2015-12-16
INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research report on the active part of the traditional Chinese medicine Forsythia being used to treat Parkinson's disease, and t...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation of fructus forsythiae active site and application of fructus forsythiae active site to Parkinson disease
  • Preparation of fructus forsythiae active site and application of fructus forsythiae active site to Parkinson disease
  • Preparation of fructus forsythiae active site and application of fructus forsythiae active site to Parkinson disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) 10kg of dried forsythia medicinal materials were crushed and extracted with 80L of 75% ethanol for 3 times, 1.5h each time; the extracts were combined and filtered, the filtrate was decompressed to recover the solvent, and the total extract of Forsythia was obtained after concentration;

[0049] (2) Use distilled water to adjust the density of the total extract of Forsythia suspensa to 1.10, and extract with 3 times the volume of petroleum ether, ethyl acetate and n-butanol successively, extract 3 times for each reagent, and combine the n-butanol solution to reduce pressure Recover the solvent and dry it to obtain the n-butanol part of Forsythia;

[0050] (3) Take the n-butanol part of Forsythia and dissolve it in water, let it stand overnight, centrifuge at 3000r / min for 20min, take the supernatant, and concentrate it to a loading solution with a concentration of 1.0g / mL. The loading solution is passed through DiaionHP-20 macroporous adsorption resin. The ratio of resin...

Embodiment 2

[0052] (1) 10kg of dried forsythia medicinal materials were crushed, and extracted with 100L of 50% ethanol for 2 times, 3h each time; the extracts were combined and filtered, the filtrate was decompressed to recover the solvent, and the total extract of Forsythia was obtained after concentration;

[0053] (2) Use distilled water to adjust the density of the total extract of Forsythia suspensa to 1.10, and extract with chloroform, ethyl acetate, and n-butanol in a volume ratio of 1 time. Extract each reagent 5 times and combine the n-butanol solution Recover the solvent under reduced pressure and dry to obtain the n-butanol part of Forsythia;

[0054] (3) Take the n-butanol part of Forsythia and dissolve it in water, let it stand overnight, centrifuge at 3000r / min for 20min, take the supernatant, and concentrate it to a loading solution with a concentration of 1.0g / mL. The loading solution is passed through AB-8 macroporous adsorption resin. The ratio of resin filling volume (mL) t...

Embodiment 3

[0056] (1) Crush 10kg of dried forsythia medicinal materials, extract 4 times with 60L of 95% ethanol, each 1h; combine the extracts and filter, and the filtrate is decompressed to recover the solvent, and the total extract of Forsythia is obtained after concentration;

[0057] (2) Use distilled water to adjust the density of the total extract of Forsythia suspensa to 1.10, and then extract with petroleum ether, chloroform and n-butanol in a volume ratio of 2 times. Extract each reagent 4 times and combine the n-butanol solution to reduce Press to recover the solvent and dry to obtain the n-butanol part of Forsythia;

[0058] (3) Take the n-butanol part of Forsythia and dissolve it in water, let it stand overnight, centrifuge at 3000r / min for 20min, take the supernatant, and concentrate it to a loading solution with a concentration of 1.0g / mL. The loading solution is passed through HPD-826 macroporous adsorption resin, the ratio of resin filling volume (mL) to loading volume (g) is...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a preparation method of a common traditional Chinese medicine of a fructus forsythiae active site. The method comprises: pulverizing a medicinal material of fructus forsythia; performing reflux extraction with a solvent, such as water or ethanol; concentrating the extracting liquid at a reduced pressure to obtain extractum; performing extraction through a plurality of organic solvents and recycling the organic solvents of the site extracted by n-butyl alcohol; dispersing and dissolving the site extracted by n-butyl alcohol in a proper amount of distilled water; taking the obtained supernate out and putting the supernate in a macroporous adsorption resin with weak polarity or medium polarity; and performing elution in a water-alcohol system to obtain the fructus forsythiae active site. According to the method, a separation method and a modern spectroscopic analysis method for a plurality of chemical components are utilized to separate and identify chemical components in the active site, thereby obtaining 15 compounds, 9 of which are new compounds. In vitro, the fructus forsythiae active site has an obvious function of preventing neurocyte damage due to rotenone, and in vivo, the fructus forsythiae active site can significantly increase the number of TH positive neuron, due to rotenone, of a substantia nigra zona compact of a rat and improve a Parkinson symptom of the rat. Therefore, the fructus forsythiae active site can be prepared into a medicine for preventing and curing the Parkinson disease.

Description

Technical field [0001] The invention relates to the active part of the traditional Chinese medicine Forsythia suspensa, its preparation method and its application in a prevention and / or Parkinson's disease, belonging to the technical field of medicine. Background technique [0002] Parkinson’s disease (Parkinson’s disease, PD) is the most common neurodegenerative disease characterized by motor dysfunction. The incidence rate of people over 65 years old is 1%. There are about 1.7 million Parkinson’s disease patients in my country. The incidence of this disease increases sharply with age. Therefore, with the intensification of the aging trend of our country's population, the number of cases will also show an upward trend year by year. PD seriously endangers human health, not only brings great pain to patients, but also causes a heavy burden to families and society. However, there is currently no effective treatment for the disease. The commonly used levodopa replacement therapy ca...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K31/7048A61P25/16A23L1/29C07H17/04C07H17/07C07H15/18C07H15/26C07H15/203C07H1/08A61K31/7032A23L33/00
CPCY02P20/582
Inventor 张培成陈乃宏张凡宋修云杨桠楠张帅冯子明姜建双
Owner INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap