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A method for degrading aflatoxin in peanut meal by black sesame solid fermentation

A kind of aflatoxin, solid fermentation technology, applied in the direction of application, medical formula, food ingredient function, etc., can solve the problems of difficult growth and reproduction of bacterial strains, unapplicable strains, and less research on degradation, etc., to achieve growth inhibition and toxin production, Ease of industrial operation and simple operation method

Active Publication Date: 2019-03-05
SHANDONG PEANUT RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on the degradation of aflatoxins in complex matrices such as peanut meal.
[0008] The strains involved in biodegradation currently mainly involve domestic Pseudoarmillaria, Myxobacteria, Stenotrophomonas maltophilia, and foreign researched Flavobacterium aurantium and Rhodococcus, etc. Growth and reproduction, low degradation rate, some strains cannot be used in food production, and there are certain safety hazards, etc.

Method used

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  • A method for degrading aflatoxin in peanut meal by black sesame solid fermentation
  • A method for degrading aflatoxin in peanut meal by black sesame solid fermentation
  • A method for degrading aflatoxin in peanut meal by black sesame solid fermentation

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Example 1 - Heizhi solid fermented peanut meal can efficiently degrade aflatoxin in peanut meal

[0044] 1. Screening of edible fungus strains that degrade aflatoxin

[0045] 1. Preliminary screening of strains producing and degrading aflatoxins

[0046]The selected strains produced different types of laccases when they were cultured on the two selection media of GLDS and NLDS. Laccase reacts differently on different selection media. When it reacts with guaiacol, it will produce a reddish-brown discoloration circle, and when it reacts with α-naphthol, it will produce a blue-black discoloration circle (such as figure 1 ), the diameter and color of the chameleon circle became bigger and darker with the increase of culture time. The color of the chromatic circle of the strain on the guaiacol medium was darker than that of the α-naphthol medium, but the diameter of the chromatic circle it produced was not as large as that on the α-naphthol medium, presumably because The...

Embodiment 2

[0066] Example 2 - After the black sesame is activated on the peanut shell medium, the activity is improved.

[0067] 10g of peanut meal was added to 10ml of water, inoculated with 1g of black sesame strain activated by peanut shells, and cultured at 30°C for 14 days; at the same time as a control, 10g of peanut meal was added to 10ml of water, and 1g of activated corn stalk was inoculated with black sesame strain. Cultivate at 30°C for 14 days; then add 20ml of distilled water, enzymatically hydrolyze at 30°C for 4 hours, measure the content of aflatoxin in the enzymolysis solution, the degradation rate of aflatoxin of the black lucidum strain activated by inoculation with peanut shells is 76.2%, while inoculation The degradation rate of aflatoxin of the black sesame strain activated by corn stalk is 62.2%, and the degradation rate of aflatoxin is increased by 22.5%.

Embodiment 3

[0068] Example 3 - Black Mushroom grows luxuriantly in peanut meal, which can inhibit the growth and toxin production of Aspergillus flavus

[0069] Add 10ml of water to 10g of peanut meal, inoculate the Heizhi strain plugged with 3 strains, and culture at 30°C for 14 days, and the growth of Heizhi in peanut meal is vigorous (see Figure 7 ); at the same time as a control, 10g of peanut meal was added with 10ml of water, without inoculation with black sesame, and cultivated at 30°C for 14 days. It is 325.9ppb.

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Abstract

The invention discloses a method for degrading aflatoxins in peanut meal by utilizing solid fermentation of fructificatio amaurodermatis rudae. The method comprises the steps of treating raw materials, activating strains, performing solid fermentation, degrading the aflatoxins, determining the aflatoxins and the like. The invention provides an efficient safe method for degrading the aflatoxins in the peanut meal. The degraded peanut meal can be used for producing and processing foods and feeds, and besides, the nutrient value of the peanut meal can also be increased. The method is simple to operate and easy in industrialization.

Description

technical field [0001] The invention relates to a method for degrading aflatoxin in peanut dregs by black sesame solid fermentation, belonging to the field of peanut food safety. Background technique [0002] my country is a large peanut producing country, and about 3 million tons of peanut meal can be obtained after peanut oil extraction every year. Peanut meal is rich in nutrients, such as peanut protein, flavonoids, sugars, ash and so on. Among them, compared with soybean protein, which is widely used, peanut protein has the advantage of containing less flatulence factors and anti-nutritional factors; compared with rapeseed and cottonseed protein, it has the advantage of containing less toxic substances. However, the utilization rate of peanut meal is low, and it is mainly used as feed, and the price is low. Now it is about 2,600 yuan / ton, which is far lower than the price of soybean meal (3,200 yuan / ton). The main factor limiting the price and widespread use is its sus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L5/20A61K36/00A23L33/00A23K10/37A23K10/12
CPCA23V2002/00A61K36/00A23V2200/30A23V2200/324A23V2200/326A23V2200/328Y02P60/87
Inventor 张建成张初署孙杰于丽娜毕洁
Owner SHANDONG PEANUT RES INST
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