Method of preparing phosphatidylserine

A technology of phosphatidylserine and serine, which is applied in the field of marine biology, can solve problems such as environmental pollution, flammable, explosive, toxic and harmful, and achieve the effects of ensuring reaction efficiency, improving production safety, and avoiding environmental pollution

Active Publication Date: 2015-12-23
威海博宇食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because the above-mentioned organic solvent has a very low boiling point, is flammable, explosive, toxic and harmful, and is likely to cause environmental pollution, it is not suitable for industrial production.

Method used

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  • Method of preparing phosphatidylserine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Extracting 20g of phospholipid raw materials with a total phospholipid content of 40% from marine animal capry roe is dissolved in 40g ethyl octanoate to obtain solution A; 0.2g activity is 80U / mg powder phospholipase D, 30gL-serine is dissolved in 40g , acetic acid buffer solution with a pH value of 5.6 to obtain solution B; add the obtained solution A to the obtained solution B to obtain a mixed solution C, heat the obtained mixed solution C to 20°C and stir for 24 hours to obtain the reacted Solution D; after the reaction, the oil phase and the water phase of the solution D are separated by a centrifugation method, and the obtained oil phase part is completely or partially removed by a molecular distillation method, and the distillation temperature in the molecular distillation is 60 ℃, the distillation pressure is 0.1~1.0Pa, the feed rate is 2.5g / min, the speed of scraping film is 150rpm, and the molecular distillation heavy phase component is collected to obtain pho...

Embodiment 2

[0058] The 20g phospholipid raw material that extracts total phospholipid content from marine animal herring roe and is 70% is dissolved in 20g fish oil ethyl ester (DHA content is 17.96%), obtains solution A; 0.3g activity is 85U / mg powder phospholipase D, 35gL - Serine was dissolved in 120g of acetic acid buffer solution with a pH value of 6.0 to obtain solution B; the obtained solution A was added to the obtained solution B to obtain a mixed solution C, and the obtained mixed solution C was heated to 30°C and stirred for 20 hours , to obtain the solution D after the reaction; after the reaction, the solution D is centrifuged to separate the oil phase and the water phase, and the obtained oil phase is partially or completely removed by molecular distillation. The distillation temperature is 70°C, the distillation pressure is 1-5Pa, the feed rate is 2.5g / min, the speed of scraping film is 150rpm, and the heavy phase component of molecular distillation is collected to obtain ph...

Embodiment 3

[0060] Extract 2g phospholipid raw material that total phospholipid content is 50% from marine animal squid ovum and be dissolved in 20g ethyl oleate, obtain solution A; 1g activity is that 95U / mg powder phospholipase D, 2gL-serine are dissolved in 300g, PH In the acetic acid buffer solution with a value of 4.5, the solution B was obtained; the obtained solution A was added to the obtained solution B to obtain a mixed solution C, and the obtained mixed solution C was heated to 50°C and stirred for 8 hours to obtain the reacted solution D After the reaction, the solution D is centrifuged to separate the oil phase and the water phase, and the obtained oil phase is partially or partially removed by molecular distillation to remove medium and long-chain fatty acid ethyl esters. The distillation temperature in the molecular distillation is 90 ° C. The distillation pressure is 5-15Pa, the feed rate is 2.5g / min, the speed of scraping film is 150rpm, and the molecular distillation heav...

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Abstract

The invention relates to a method of preparing phosphatidylserine. The method comprises the following steps: taking phospholipase D to catalyze a basic group exchange reaction of marine animal phospholipid and L-serine; centrifuging and carrying out molecular distillation, and separating and purifying to prepare the phosphatidylserine. By utilizing a liquid-liquid double-phase reaction system formed by middle-chain and long-chain fatty acid ethyl esters and a buffering solution, and utilizing the middle-chain and long-chain fatty acid ethyl esters which have high boiling points and are safe to eat to replace the organic solvents including diethyl ether, ethyl acetate and the like which have low boiling points, are flammable and combustible and are toxic and harmful, the production safety is improved while the reaction efficiency is guaranteed and the environmental pollution is not caused, so that the method is suitable for industrial production.

Description

Technical field: [0001] The invention relates to the technical field of marine organisms, in particular to a method for preparing phosphatidylserine. Background technique: [0002] Phosphatidylserine (PS) is the only phospholipid that can regulate the function of key proteins in the cell membrane. It has an important regulatory effect on many cell metabolism processes. The brain function of the elderly has a good curative effect, and it has a very significant effect on repairing brain damage and treating ADHD in children; in addition, PS can promote the recovery of brain fatigue, balance emotions, and relieve depression. [0003] Phosphatidylserine exists in the biofilms of all animals, higher plants and microorganisms, and is one of the important components of cell membrane phospholipids. Human nerve cell membranes contain a large amount of phosphatidylserine, and its glycerol skeleton is rich in DHA (docosahexaenoic acid), which plays an important role in the maintenance,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/04C07F9/10
Inventor 蔡胜利蔡爱英孙兆敏黄莎莎李文平
Owner 威海博宇食品有限公司
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