SNP (single-nucleotide polymorphism) marker of wheat grain weight gene TaGS5-3A and application of SNP marker
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A single nucleotide polymorphism and nucleotide technology, applied in the field of wheat grain weight gene TaGS5-3A single nucleotide polymorphism markers, can solve the problem of single grain type/few grain weight genes
Active Publication Date: 2016-01-06
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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In the field of wheat grain type/grain weight research, due to the huge and complex genome structure of wheat, although a large number of QTLs related to grain type/gr...
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Embodiment 1
[0057] Example 1, the excavation of the SNP site of TaGS5-3A gene and the development of its detection marker
[0058] 1. Discovery of SNP loci of TaGS5-3A gene among wheat cultivars
[0059] 1. Wheat material for testing
[0060] 36 wheat materials (coded POL1-36) distributed in different wheat regions in my country with large differences in grain traits were selected as excavation materials for polymorphic loci (Table 1);
[0061] 2. Sequence alignment of TaGS5-3A gene
[0062] The genomic DNA of the above wheat materials was extracted by conventional CTAB method, and the TaGS5-3A gene region of 36 wheat materials was amplified respectively, and the amplification primers used were TaGS5-3A-F and TaGS5-3A-R.
[0063] TaGS5-3A-F: 5'-CCCTCCAACTTCACATG-3';
[0064] TaGS5-3A-R: 5'-TCATCGGTGTGTAGGAAG-3'.
[0065] After amplification, agarose gel electrophoresis was performed, and the amplified fragments were recovered and sequenced; the sequences were spliced using Seqman in...
Embodiment 2
[0091] Example 2, Functional Verification of TaGS5-3A Gene SNP Site
[0092] The functional verification of TaGS5-3A gene was carried out by using Chinese wheat micro-core collection (HouJ, et al. Global selection on sucrose synthase haplotypes during a century of wheat breeding. Plant Physiol 2014; 164: 1918-1929), combined with thousand-kernel weight (TKW), grain length (KL), grain width (KW), grain Thickness (KT) and other multi-year multi-point phenotypic data for marker / trait association analysis. Wherein, the method for identifying the genotype of TaGS5-3A gene refers to Example 1. The basic information of all wheat micro-core germplasm materials in this example and the grain phenotype identification data in Luoyang, Henan in 2002 are shown in Table 2 (in addition, corresponding experiments were also carried out in Luoyang, Henan in 2005 and Shunyi, Beijing in 2010) . When measuring the grain phenotype data, at least 3 repeated measurements were set for each sample, and ...
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Abstract
The invention discloses an SNP (single-nucleotide polymorphism) marker of a wheat grain weight gene TaGS5-3A and an application of the SNP marker. The application specifically refers to the application of the SNP at a following SNP locus in a wheat genome to identification or auxiliary identification of related traits (grain weight and/or grain length and/or grain width and/or grain thickness) of to-be-detected wheat grains, wherein the SNP locus refers to the 718th nucleotide from 5' end of an amplification product obtained through PCR (polymerase chain reaction) amplification performed with DNA (deoxyribonucleic acid) of the wheat genome as a template and with adoption of a primer pair, and the nucleotide at the SNP locus is T or G; the primer pair is formed by a single-stranded DNA shown in a sequence 1 in a sequence table and a single-stranded DNA shown in a sequence 2 in the sequence table. Research findings show that a TaGS5-3A-T allele has significant or very significant positive correlation with the wheat grain weight, grain length, grain width and grain thickness; meanwhile, the SNP marker is a codominant marker, has good repeatability and low cost and has broader application prospect in wheat assisted selection and molecular design breeding.
Description
technical field [0001] The invention belongs to the field of biotechnology, and relates to a wheat grain weight gene TaGS5-3A single nucleotide polymorphism marker and application thereof. Background technique [0002] Food crops such as wheat, rice, and corn provide about 50% of the energy required by humans. However, with the growth of the world's population, the reduction of arable land and the increase of food production costs, improving the yield of food crops has always been the goal of breeders. main purpose. Wheat is an important food crop in my country, and its cultivation area is second only to rice, the largest food crop. The level of wheat production directly affects the living standards of our people and national food security. Therefore, the use of molecular biology techniques to clone functional genes related to wheat yield and develop corresponding molecular markers can provide important genetic resources for wheat molecular marker-assisted breeding, which w...
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