Protein capable of improving salt tolerance and drought tolerance of plants as well as coding gene and application of protein

A drought tolerance and plant technology, applied in the direction of plant genetic improvement, application, plant peptides, etc., can solve the problem that the mechanism of action remains to be elucidated

Inactive Publication Date: 2016-01-20
山西尧山种业科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it is generally believed that the PMP3 gene is indirectly involved in th

Method used

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  • Protein capable of improving salt tolerance and drought tolerance of plants as well as coding gene and application of protein
  • Protein capable of improving salt tolerance and drought tolerance of plants as well as coding gene and application of protein
  • Protein capable of improving salt tolerance and drought tolerance of plants as well as coding gene and application of protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1, cultivate transgenic rice

[0046] 1. Construction of recombinant expression vector

[0047] 1. Cloning of MePMP3-2 gene

[0048] ① Extraction of cassava total RNA

[0049] After the plant tissue material is collected, cut it into pieces, put it in liquid nitrogen and grind it into a dry powder quickly, take about 100 mg of the powder material, put it into a 1.5 mL centrifuge tube filled with 1.0 mL TRIzol extract (Invitrogen) in advance, and cover it tightly Cap the tube and shake to mix the sample and TRIzol extract thoroughly. After numbering with a low-temperature marker pen, store in a -70°C ultra-low temperature freezer for future use.

[0050] Take out the samples frozen in the ultra-low temperature refrigerator at -70°C for 5 minutes to completely lyse the nucleoprotein of the samples, add an appropriate amount of chloroform according to 200 μl chloroform / ml TRIzol, and shake vigorously for 15 seconds (note: Vortex shaker is not allowed here, I...

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Abstract

The invention discloses a protein capable of improving salt tolerance and drought tolerance of plants as well as a coding gene and application of the protein. The protein has a sequence represented by SEQ ID NO.1 and is named as MePMP3-2. The coding gene of the protein has a sequence represented by SEQ ID NO.2 and is named as gene MePMP3-2. The protein coded by the gene MePMP3-2 is positioned in cell membranes of plants, and the coding gene MePMP3-2 of the protein is introduced into the plants, so that the germination rate of seeds of the transgenic plants under the treatment of ABA, NaCl and mannitol is increased, the root lengths and the seedling lengths of the transgenic plants under high-salinity and high-mannitol under adversities are increased, and the transgenic plants are relatively high in survival rate, relatively low in malondialdehyde (MDA) content and relatively high in proline (Pro) content under the stress of drought and high salinity; the relative expression quantities of adversity relevant genes including OsProT, OsP5CS, OsDREB2A, OsLEA3 and the like in the transgenic plants are increased at different degrees under adversity stress.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a protein for improving salt and drought tolerance of plants, its coding gene and application. Background technique [0002] Abiotic stresses, such as high temperature, drought, high salinity, alkaline soil, heavy metals, and peroxidation stress, all seriously threaten agricultural production and are the main cause of crop yield reduction worldwide. The growth and development of plants, as well as the yield, quality and later growth of crops are all affected by abiotic stresses, and 60% of the production reduction of major crops worldwide is caused by abiotic stresses. In order to ensure the sustained and stable high yield of crops, humans urgently need to breed new crop varieties with higher tolerance to abiotic stresses such as drought, high salinity, and peroxidation. However, the progress of improving the traits and stress resistance of crops through traditional breeding methods...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00
CPCC07K14/415C12N15/8273
Inventor 余艳段艳平黄和芳
Owner 山西尧山种业科技有限公司
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