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A preparation method of cartilage inductive matrix for microfracture surgery

A technique of microfracture and surgery, applied in medical science, prostheses, vertebrate cells, etc., can solve problems such as irreparable damage, easy loss of bone marrow cells, and impact on the quality of life of patients, achieving long-term maintenance of joint function and simple and convenient operation , the effect of reducing cell density

Active Publication Date: 2016-09-21
SHANGHAI BIOMED UNION BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantages are one-time operation, minimally invasive, simple, economical, etc.; the disadvantages are that bone marrow cells are easily lost, large-scale damage cannot be repaired, and hyaline cartilage cannot be formed.
In fact, the cartilage defect repaired by microfracture surgery can only form fibrocartilage at the defect site due to the above reasons, and cannot restore the defect cartilage to its normal shape and function, which has a serious impact on the patient's quality of life

Method used

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  • A preparation method of cartilage inductive matrix for microfracture surgery
  • A preparation method of cartilage inductive matrix for microfracture surgery
  • A preparation method of cartilage inductive matrix for microfracture surgery

Examples

Experimental program
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Effect test

Embodiment 1

[0022] This embodiment provides a cartilage-inducing matrix for microfracture surgery, which can fix bone marrow cells in microfracture surgery, cover the defect area, and induce bone marrow cells to develop in the direction of hyaline cartilage, so as to meet the clinical application of many patients. Its joint function is maintained for a long time.

[0023] A cartilage-inducing matrix for microfracture surgery, including gel and additives, the gel is fibrin 100mg / ml; collagen 25mg / ml; hyaluronic acid 5%; thrombin 50U / ml; Cover the cartilage defect and solidify within 5 minutes to provide a fixed scaffold for bone marrow cells; the ingredients of the additives in the medium are based on the final concentration, including: vitamin C: 233μM-287μM; linoleic acid: 3.7μM-8.9μM; cholesterol : 9μM~17μM; Dexamethasone: 6nM~15nM; Acetylcysteine: 43μM~58μM; Transferrin: 18μg / mL~32μg / mL; Sodium Selenite: 22nM~52nM; Sodium Pantothenate: 13μM~24μM ; Biotin: 28μM~43μM; Insulin-like growt...

Embodiment 2

[0027] This embodiment provides a cartilage-inducing matrix for microfracture surgery, which can fix bone marrow cells in microfracture surgery, cover the defect area, and induce bone marrow cells to develop in the direction of hyaline cartilage, so as to meet the clinical application of many patients. Its joint function is maintained for a long time.

[0028]A cartilage-inducing matrix for microfracture surgery, including gel and additives, the gel is fibrin 100mg / ml; collagen 25mg / ml; hyaluronic acid 5%; thrombin 50U / ml; Cover the cartilage defect and solidify within 5 minutes to provide a fixed scaffold for bone marrow cells; the ingredients of the additives in the medium are based on the final concentration, including: vitamin C: 250 μM; linoleic acid: 4.5 μM; cholesterol: 13 μM; Methasone: 10nM; Acetylcysteine: 50μM; Transferrin: 25μg / mL; Sodium Selenite: 30nM; Sodium Pantothenate: 17μM; Biotin: 33μM; Insulin-like Growth Factor: 10μg / mL; Epidermal Growth Factor: 5ng / mL; ...

Embodiment 3

[0032] A method for preparing a cartilage-inducing matrix for microfracture surgery, comprising the steps of:

[0033] Step 1: Add vitamin C, linoleic acid, cholesterol, dexamethasone, acetylcysteine, transferrin, sodium selenite, sodium pantothenate, biotin, insulin-like growth factor, epidermal growth factor, fibroblast growth Factor, platelet-derived growth factor, transforming growth factor, insulin transferrin were added to the basal medium so that the final concentration of the additive in the basal medium was:

[0034] Vitamin C: 233μM~287μM; Linoleic acid: 3.7μM~8.9μM; Cholesterol: 9μM~17μM; Dexamethasone: 6nM~15nM; Acetylcysteine: 43μM~58μM; Transferrin: 18μg / mL~32μg Sodium selenite: 22nM~52nM; Sodium pantothenate: 13μM~24μM; Biotin: 28μM~43μM; Insulin-like growth factor: 7μg / mL~18μg / mL; Epidermal growth factor: 2ng / mL~9ng / mL ; Fibroblast growth factor: 2ng / mL~9ng / mL; Platelet-derived growth factor: 2ng / mL~9ng / mL; Transforming growth factor: 30ng / ml~70ng / ml;

[0035...

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Abstract

The invention provides a cartilage-inducing matrix for microfracture surgery and a preparation method thereof. The cartilage-inducing matrix includes gel and additives, the gel is fibrin, collagen, hyaluronic acid, and thrombin; the additive is in the culture medium The final concentration of vitamin C: 233μM-287μM; linoleic acid: 3.7μM-8.9μM; cholesterol: 9μM-17μM; dexamethasone: 6nM-15nM; acetylcysteine: 43μM-58μM; transferrin: 18μg / mL~32μg / mL; sodium selenite: 22nM~52nM; sodium pantothenate: 13μM~24μM; biotin: 28μM~43μM; insulin-like growth factor: 7μg / mL~18μg / mL; epidermal growth factor: 2ng / mL~9ng / mL; fibroblast growth factor: 2ng / mL~9ng / mL; platelet-derived growth factor: 2ng / mL~9ng / mL; transforming growth factor: 30ng / ml~70ng / ml; 1%.

Description

technical field [0001] The invention relates to the field of cartilage repair, in particular to a method for preparing a cartilage-inducing matrix used in microfracture surgery. Background technique [0002] Articular cartilage damage, whether caused by trauma or pathology, cannot be repaired spontaneously, and usually leads to degenerative changes of cartilage, which in turn leads to osteoarthritis. At present, exogenous repair methods are mainly used, such as osteochondral transplantation and microfracture. In recent years, cartilage tissue engineering technology is considered to be the only way to repair large areas of cartilage damage, but the main method of cartilage tissue engineering is to cultivate seed cells in vitro to construct tissue engineered cartilage, which often requires two operations. Seed cells, tissue engineered cartilage transplantation for the second time, the in vitro construction period is as long as 2-3 weeks, the operation is difficult, the loss r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071A61L27/50
Inventor 柴勋吴国祥李文琳袁彩艳韩武梅张易
Owner SHANGHAI BIOMED UNION BIOTECHNOLOGY CO LTD
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