A kind of rapid propagation method of allium plant bulb plate
A bulb disc and fast technology, which is applied in the agricultural field, can solve the problems of difficulty in meeting large-scale production and low proliferation rate of tissue culture of Allium plants, and achieves the effects of low price cost, low time cost and promotion of development.
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Embodiment 1
[0032] Embodiment 1 Tiller onion stem tip multiplication
[0033] The shoot tip of tillering onion was inoculated in different concentrations of 6-BA, NAA combination and 6-BA, IAA combination medium (Table 1). And NAA in the induction of proliferation, there is a big difference. Among them, the proliferating seedlings induced by the combination of 6-BA and NAA hormones grow better, and have a higher differentiation coefficient. After 4 weeks of culture, the average proliferation coefficient can reach 3.87; although the combination of 6-BA and IAA hormones can also achieve shoot tip Proliferation, but the proliferated seedlings obtained are thin and weak, with low differentiation coefficient, and albino seedlings appear (such as figure 1 ). It can be seen that the effect of IAA is not as good as that of NAA. Therefore, MS+6-BA0.3mg / L+NAA0.1mg / L medium was determined to be the best medium for shoot tip proliferation.
[0034]
[0035] Note: MS is the basic medium.
Embodiment 2
[0036] Embodiment 2 Tiller onion bulb disc multiplication
[0037] Take one tillering onion, remove the corky part of the tillering onion bulb disk, keep the bulb disk with a diameter of 0.4-1 cm centered on the central growth point and the bulb wrapped on the upper part, and obtain the center part of the tillering onion. Rinse the center of the tillering onion with running water for 0.5 h, soak and disinfect with 75% alcohol for 1 min, then soak and disinfect with 1% NaClO solution for 15-20 min, rinse with sterile water 3-5 times to remove residual NaClO, absorb with filter paper Dry water backup (such as figure 2 ).
[0038]Then remove the upper bulb, keep the bulb disk including the main growth point, cut the bulb disk into 4 parts, remove the main growth point and the main growth point bulb disk, and inoculate them on MS as the basal medium respectively, add 6-BA ( 0.2-0.8 mg / L), NAA (0.1-2. 0mg / L), and cultured in the value-proliferating medium whose pH was adjusted t...
Embodiment 3
[0043] Example 3 Test tube balling of tillering onion virus-free seedlings
[0044] Take the tip of the tiller onion, wash it with running water for half an hour, soak it in 75% alcohol for 1 minute, then soak it in 1% NaClO solution for 15-20 minutes, rinse it with sterile water for 3-5 times to remove the residual NaClO, and absorb it with filter paper. Dry the water for backup, peel off the part of the growth point of the tiller onion shoot tip <0.3mm under the microscope, and induce seedlings in the starting medium with MS as the base medium and supplemented with 6-BA and IAA.
[0045] After the stem tips became seedlings, sucrose was used as the basic carbon source to set 5 carbon source sugar concentrations of 30mg / L, 45mg / L, 60mg / L, 75mg / L, and 90mg / L, to explore the effects of different sugar concentrations on the tillering onion test-tube plantlets. For the effect of bulbing, the sugar concentration in the best bulbing medium was screened, and it was found through cul...
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